Tia1 (BC023813) Mouse Untagged Clone

CAT#: MC217759

Tia1 (untagged) - Mouse cytotoxic granule-associated RNA binding protein 1 (cDNA clone MGC:36034 IMAGE:5353938), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tia1
• Synonyms: 2310050N03Rik; AI256674; mTIA-1; TIA-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC023813
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGACGAGATGCCCAAGACTCTATACGTCGGTAACCTTTCTCGAGATGTGACAGAAGCTCTCATCC
  TCCAGCTCTTTAGCCAGATTGGACCTTGTAAAAACTGCAAAATGATTATGGATACAGCAGGAAATGACCC
  ATACTGTTTTGTGGAGTTCCATGAGCACCGTCATGCAGCTGCAGCCCTGGCTGCCATGAATGGGCGGAAG
  ATAATGGGTAAGGAAGTGAAAGTGAATTGGGCAACAACCCCTAGCAGTCAAAAGAAGGATACGAGCAATC
  ATTTCCATGTGTTTGTTGGTGACCTCAGTCCAGAAATCACAACCGAAGACATCAAAGCAGCGTTTGCACC
  ATTTGGAAGAATTTCAGATGCCCGTGTGGTAAAAGACATGGCTACCGGGAAGTCTAAGGGATATGGCTTT
  GTCTCCTTTTTCAACAAATGGGATGCAGAAAATGCCATTCAGCAGATGGGTGGCCAGTGGCTTGGTGGAA
  GACAAATCAGAACTAACTGGGCAACCCGAAAGCCTCCAGCTCCAAAGAGTACATATGAGTCGAACACCAA
  ACAGCTGTCATATGATGAGGTTGTGAGTCAGTCTAGTCCCAACAACTGCACTGTGTACTGTGGAGGGGTG
  ACGTCAGGACTGACAGAACAACTAATGCGTCAGACTTTTTCTCCATTTGGACAAATAATGGAAATTCGAG
  TCTTCCCAGATAAAGGATACTCATTTGTTCGTCAGACCCAGTCTTCTGCACCGTGGATGGGACCCAATTA
  CAGTGTGCCACCACCTCAAGGGCAGAATGGCAGCATGTTGCCTAGTCAGCCTGCTGGGTATCGAGTGGCC
  GGGTATGAAACCCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC023813
• Insert Size: 858 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC023813, AAH23813
• RefSeq Size: 1455 bp
• RefSeq ORF: 857 bp
• Locus ID: 21841
• Cytogenetics: 6 D1
• Gene Summary: Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis (By similarity).[UniProtKB/Swiss-Prot Function]