Scg3 (NM_001164790) Mouse Untagged Clone

CAT#: MC217744

Scg3 (untagged) - Mouse secretogranin III (Scg3), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Scg3
• Synonyms: 1B1075; AI385542; Chgd; SgIII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217744 representing NM_001164790
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTTCCTTTGGACCGGCTCTTGGATACTGGTGTTGGTGCTCAACAGCGGCCCAATTCAAGCTTTCC
  CCAAACCCGAAGGCAGCCAAGACAAATCCCTGCATAATAGAGAATTAAGTGCAGAAAGACCTTTGAATGA
  ACAGATCGCTGAGGCAGAGGCAGACAAGATTAAAAAGGCATTCCCTTCAGAAAGCAAGCCGAGTGAAAGC
  AATTATTCTTCTGTCGATAACTTGAATCTGCTGAGGGCAATAACAGAAAAGGAAACCGTTGAGAAAGAGA
  GACAATCCATAAGAAGCCCCCCGTTTGATAACCAACTGAACGTGGAAGACGCTGATTCAACCAAAAATCG
  GAAACTGATCGATGAGTACGATTCCACCAAGAGTGGACTGGACCACAAGTTTCAAGATGACCCAGACGGC
  CTTCATCAACTGGATGGAACTCCTTTAACTGCTGAAGACATCGTCCATAAGATTGCCACCAGGATTTATG
  AGGAGAACGACAGAGGAGTGTTTGACAAAATTGTTTCTAAACTGCTGAATCTTGGCCTGATCACTGAAAG
  CCAGGCACATACTCTGGAAGATGAAGTAGCAGAAGCTTTACAAAAACTGATTTCAAAAGAGGCCAACAAT
  TATGAGGAGACCCTGGATAAACCCACAAGCAGGACCGAGAATCAGGATGGGAAAATACCAGAGAAAGTGA
  CTCCGGTGGCAGCAGTCCAAGATGGCTTCACTAACCGTGAAAACGATGAGACGGTGTCTAACACCTTGAC
  CTTGTCCAATGGCTTGGAAAGGAGAACTAACCCCCACAGGGAAGACGACTTTGAGGAACTCCAGTATTTC
  CCCAACTTCTATGCACTACTGACAAGCATCGACTCAGAAAAAGAAGCAAAAGAGAAAGAAACCCTGATCA
  CCATCATGAAGACATTGATTGACTTCGTGAAAATGATGGTGAAATACGGTACGATATCTCCAGAGGAAGG
  CGTGTCCTACCTTGAAAACTTGGATGAAACAATTGCTCTGCAGACCAAGAACAAGCTAGAAAAAAATACT
  ACTGATAGCAAAACTCCACCAGAGAAGAGTCAGGAAGAAACAGACAGTACCAAGGAAGAAGCCGCCAAGA
  TGGAAAAGGAATACGGAAGCCTAAAAGACTCTACAAAAGATGATAACTCCAACCTAGGAGGAAAGACAGA
  TGAAGCCACAGGGAAGACAGAAGCCTACTTGGAAGCCATTAGAAAAAACATCGAATGGCTGAAGAAACAT
  AACAAGAAGGGCAACAAAGAAGATTACGACCTTTCAAAGATGAGGGACTTTATCAACCAACAAGCTGACG
  CTTATGTGGAGAAGGGCATCCTCGACAAGGAAGAAGCCAACGCCATCAAACGCATCTACAGCAGCCTGTG
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001164790
• Insert Size: 1401 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164790.1, NP_001158262.1
• RefSeq Size: 2192 bp
• RefSeq ORF: 1401 bp
• Locus ID: 20255
• UniProt ID: P47867
• Cytogenetics: 9 42.32 cM
• Gene Summary: Member of the granin protein family that regulates the biogenesis of secretory granules (PubMed:16219686). Acts as a sorting receptor for intragranular proteins including chromogranin A/CHGA (PubMed:12388744). May also play a role in angiogenesis (PubMed:28330905). Promotes endothelial proliferation, migration and tube formation through MEK/ERK signaling pathway (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to variant 1.