Pnp (BC052679) Mouse Untagged Clone

CAT#: MC217733

Pnp (untagged) - Mouse purine-nucleoside phosphorylase (cDNA clone MGC:60531 IMAGE:30062908), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pnp
• Synonyms: AL024301; AU015798; Np; Np-1; Np-2; Pnp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC052679
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAACGAGTTCACATACGAAGATTATGAGACCACTGCCAAGTGGCTTCTGCAACACACTGAATATC
  GACCTCAAGTGGCAGTGATCTGTGGTTCCGGCTTAGGAGGGCTGACTGCTCACTTAAAGGAGGCTCAGAT
  CTTTGACTACAATGAGATACCCAACTTTCCCCAAAGCACAGTGCAAGGTCACGCAGGCCGACTGGTGTTT
  GGATTGCTGAATGGCAGATGCTGTGTGATGATGCAAGGCCGGTTCCATATGTATGAAGGATACTCACTGT
  CAAAGGTGACATTCCCAGTGAGAGTTTTCCATCTTCTGGGTGTGGAAACTTTGGTGGTCACCAATGCTGC
  TGGAGGACTCAACCCTAATTTTGAAGTTGGAGATATTATGCTGATCCGTGATCACATCAACCTACCTGGT
  TTCTGTGGCCAGAACCCTCTCCGGGGCCCCAACGATGAAAGGTTTGGAGTTCGTTTTCCTGCCATGTCTG
  ATGCTTATGACCGGGATATGAGGCAGAAGGCTTTCACTGCCTGGAAACAAATGGGGGAGCAACGAAAGCT
  ACAAGAAGGCACCTACGTGATGTTGGCAGGCCCCAACTTTGAGACTGTGGCAGAGAGTCGTCTGCTAAAG
  ATGCTGGGAGCAGATGCTGTTGGCATGAGCACAGTCCCAGAAGTTATCGTCGCAAGGCACTGTGGGCTTC
  GAGTCTTTGGTTTCTCACTCATTACGAACAAGGTTGTCATGGATTATGAGAACTTGGAGAAGGCCAATCA
  CATGGAAGTTCTGGATGCCGGGAAAGCAGCTGCACAGACATTGGAAAGGTTTGTCTCCATTCTTATGGAG
  AGCATTCCACTCCCGGATCGTGGCAGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC052679
• Insert Size: 870 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052679, AAH52679
• RefSeq Size: 1305 bp
• RefSeq ORF: 869 bp
• Locus ID: 18950
• Cytogenetics: 14 26.31 cM
• Gene Summary: The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.[UniProtKB/Swiss-Prot Function]