Pim2 (BC027376) Mouse Untagged Clone

CAT#: MC217731

Pim2 (untagged) - Mouse proviral integration site 2 (cDNA clone MGC:37188 IMAGE:4954711), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pim2
• Synonyms: DXCch3; Pim-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC027376
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGACCAAGCCTCTGCAGGGGCATCCTTCGCCCCCTGTGACCCCCACGCAGCCTCCAGGAGGCAAGG
  ATCGGGCAGCTTTCGAGGCCGAATACCGACTTGGCCCCCTCCTGGGTAAGGGAGGCTTTGGCACCGTCTT
  CGCGGGACACCGCGTCACGGATAGACGTCAGGTGGCCATCAAAGTAATCTCCCGGAACCGTGTGCTAGGC
  TGGTCCACCGTGTCAGACTCAGTCACCTGCCCACTTGAGGTTGCGCTGCTGTGGAAGGTGGGTGAAGGCA
  ATGGCCATCCGGGTGTGATACGCCTTCTTGACTGGTTCGAAACACCCGAAGGCTTCATGCTGGTCCTTGA
  GCGGCCTATGCCTGCTCAGGATCTCTTCGACTATATCACAGAGAAGGGGCCGCTGGGTGAAAGCTGTAGC
  CGCAGCTTCTTTACCCAAGTCGTGGCAGCTGTCCAGCACTGCCACGCCCGTGGAGTTGTCCATCGGGATA
  TCAAGGATGAGAACATCCTGATCGACCTATGCCGGGGTTCCATTAAACTCATTGATTTTGGTTCCGGCGC
  CCTGCTTCACGATGAGCCGTACACTGACTTTGATGGGACAAGAGTGTATAGCCCTCCAGAGTGGATCTCG
  CGACACCAGTACCATGCCCTGCCAGCGACCGTCTGGTCACTAGGTGTCCTACTCTATGACATGGTCTGTG
  GGGACATTCCCTTCGAGAGAGACCAGGAGATTCTGGAGGCTGAGCTGCACTTCCCTGCTCATGTCTCCCC
  AGATTGCTGTGCCCTAATCCGCCGGTGCCTGGCCCCTAAACCCTGCTCCCGACCCTCACTGGAGGAGATT
  CTGCTGGACCCCTGGATGCAATCACCAGCTGAAGAAAAGCCCATCAACTCCTCCAAAGGAAGCCCCACCC
  CCTTGCCCTGGTCCCTGCTTCCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC027376
• Insert Size: 936 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC027376, AAH27376
• RefSeq Size: 1974 bp
• RefSeq ORF: 935 bp
• Locus ID: 18715
• Cytogenetics: X 3.55 cM
• Gene Summary: Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression, the regulation of cap-dependent protein translation and through survival signaling by phosphorylation of a pro-apoptotic protein, BAD. Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM2 might explain partly the strong synergism between these 2 oncogenes in tumorigenesis. Regulates cap-dependent protein translation in a mammalian target of rapamycin complex 1 (mTORC1)-independent manner and in parallel to the PI3K-Akt pathway. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappa-B kinase/NF-kappa-B cascade; this process requires phosphorylation of MAP3K8/COT. Promotes growth factor-independent proliferation by phosphorylation of cell cycle factors such as CDKN1A and CDKN1B. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.[UniProtKB/Swiss-Prot Function]