Pctk1 (BC011069) Mouse Untagged Clone

CAT#: MC217728

Pctk1 (untagged) - Mouse PCTAIRE-motif protein kinase 1 (cDNA clone MGC:18746 IMAGE:4006745), (10ug)


  "BC011069" in other vectors (4)

Reconstitution Protocol

USD 503.00

2 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (3)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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Other products for "Pctk1"

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Pctk1
Synonyms Crk5; Pctaire1; Pctk1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC011069
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCGGATGAAGAAGATCAAACGGCAGCTGTCAATGACACTCCGAGGGGGCCGAGGCATAGATAAGA
CCAATGGTGTCCCTGAGCAGATAGGCCTGGATGAGAGTGGTGGCGGTGGTGGCAGTGACCTTGGAGAAGC
TCCCACCCGTATTGCCCCTGGGGAGCTTCGCTCTGTTCGGGGCCCACTCAGCTCTGCACCAGAGATTGTG
CACGAGGACATGAAGATGGGATCTGATGGGGAGAGTGACCAGGCTTCAGCCACATCCTCAGATGAGGTGC
AGTCTCCAGTGAGAGTGCGCATGCGCAACCACCCCCCACGCAAGATCTCCACTGAGGACATCAACAAACG
CCTGTCACTACCAGCTGACATACGGCTGCCTGAGGGCTACCTTGAGAAGCTGACCCTCAATAGCCCCATC
TTTGATAAGCCTCTTAGCCGGCGCCTCCGCCGAGTCAGTTTGTCTGAGATTGGCTTTGGAAAACTGGAGA
CCTACATCAAGCTAGACAAGCTGGGTGAGGGTACCTATGCCACTGTCTACAAAGGCAAAAGCAAGCTCAC
AGACAACCTTGTAGCACTTAAGGAGATCAGACTGGAACACGAAGAAGGGGCACCCTGTACTGCTATCCGG
GAAGTATCCCTGCTTAAGGACCTCAAGCATGCCAACATCGTCACACTACATGACATTATCCACACAGAGA
AGTCCCTCACACTTGTCTTTGAATACTTGGACAAGGACCTGAAGCAGTACCTGGATGACTGTGGAAATGT
CATCAACATGCACAATGTGAAACTGTTCCTGTTCCAGTTGCTCCGTGGCCTGGCCTACTGCCACAGGCAG
AAGGTGCTACACCGAGACCTCAAGCCCCAGAACCTACTCATCAACGAGAGGGGAGAGCTCAAACTGGCAG
ATTTTGGCCTGGCTCGTGCCAAGTCAATTCCTACTAAAACATACTCCAACGAAGTGGTGACACTGTGGTA
CCGGCCCCCTGACATCCTACTTGGGTCCACAGACTACTCTACCCAAATTGACATGTGGGGTGTAGGCTGC
ATCTTCTATGAGATGGCCACCGGCCGGCCCCTCTTCCCAGGCTCCACAGTGGAAGAACAGCTGCACTTCA
TCTTCCGCATTTTGGGAACCCCAACTGAGGAGACATGGCCAGGTATCCTGTCCAATGAGGAGTTTAGAAC
ATACAACTACCCCAAGTACCGAGCCGAGGCCCTTCTGAGCCATGCACCCCGACTTGATAGCGATGGAGCT
GACCTCCTCACCAAGCTGCTGCAGTTTGAGGGTCGCAATCGGATCTCTGCTGAGGATGCCAGGAAACATC
CATTCTTTCTCAGCTTGGGGGAGCGGATCCACAAACTTCCTGACACTACTTCCATATTTGCACTAAAGGA
GGTACAGCTACAAAAGGAGGCCAACATTCGGTCCACTTCTATGCCTGACTCAGGCAGGCCAGCTTTCCGT
GTGGTGGATACCGAGTTCTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Restriction Sites SgfI-MluI     
ACCN BC011069
Insert Size 1491 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC011069, AAH11069
RefSeq Size 2815 bp
RefSeq ORF 1490 bp
Locus ID 18555
Cytogenetics X 16.18 cM
Gene Summary Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Can phosphorylate CCNY at 'Ser-336' (in vitro) (By similarity). Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Regulates GH1 release by brain neurons. Phosphorylates NSF, and thereby regulates NSF oligomerization. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development.[UniProtKB/Swiss-Prot Function]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.