E4f1 (BC071228) Mouse Untagged Clone

CAT#: MC217697

E4f1 (untagged) - Mouse E4F transcription factor 1 (cDNA clone MGC:78185 IMAGE:4915902), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: E4f1
• Synonyms: p120E4F; phi-AP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC071228
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGACGATGTGCACAGATGTGGCCGCTGCCAAGTGGAATTCACTGCCTTGGAGGACTTTGTCCAGCA
  CAAGATTCAGAAGACCTGCCATCGGGCCCCGCAGGAGGCCCTGCCCACCACCCCTTGCCACTGCACTGCT
  GGACCAGGAGGTGGTGCCCACAGCAGCAGAGGGAGGTCCAGATGAACCCATCACTGTGGCTCACATCGTG
  GTGGAGGCCACCTCTCTAGCAGAAGACATCAGCCACGCTCCTGACCTTGTTGGAAGTGGGCACATCAAAG
  AGGTCATTGTGGCTGCTGAGGCAGAGCCAGGGGATGGCGAGATGGCAGAGGCTCCAGGCAGTCCTAATCA
  TCAGGAACTTGGGCTTCTTGGGGAGGGCGAGCAGGCCCATGTCAAGCTGCTGGTGAACAAGGAAGGCCGC
  TACGTGTGCATGCTATGTCACAAGACCTTCAAAACGGGCAGCATCCTCAAGGCCCACATGGTAACGCACA
  GCAGCCGCAAGGACCACGAGTGCAAGCTCTGTGGGGCCTCTTTTCGGACCAAGGGCTCTCTCATCCGGCA
  CCACCGACGGCACACTGATGAGCGCCCCTACAAATGTGCCAAGTGTGGAAAGAGTTTCCGAGAGTCAGGC
  GCACTGACTCGGCACCTCAAATCTCTCACTCCATGCACAGAAAAGATCCGCTTCAGCATAAGCAAGGACA
  CAGCTGTGGGCAAAGAGGAAGTGCCTGCAGGGTCCAGTGCCTCCACTGTGGGGACGGTGACATCATCAGT
  GGCAGGAGACCCCATGGAGACATCACCTGTGATTCACCTGGTGACAGATGCCAAGGGTACTGTCATCCAT
  GAAGTCCACGTCCAGATGCAGGAGCTTCCCCTGGGCATGAAAGCCCTGACCCCAGAGTCCCCAGACTCGG
  AGGAGCTCCCCTGTTCCAGTGAGAACAGCCGTGAGAACCTGCTACATCAGGCCATGCAGAATTCTGGCAT
  CGTCCTTGAGAGGGTTGCTGGAGAGGAGAGTGCTCTGGAGCCAGCCCCTCCCTCTGGGTCCAGTCCCCAG
  TGCCTGGGAGATGGATCCCCTGAACTGCCTCTGCTGAAGGTGGAGCAGATAGAGACACAGGTGGCCAGTG
  AGGCCGCCACCGTGCCCAGGACCCACCCGTGCCCTCAGTGCAGTGAGACTTTCCCAACAGCAGCCACGCT
  GGAGGCCCACAAGAGAGGTCACATAGTGTGGCAAGGCCTTCCCCAAAGCCTACCTGCTCAAGAAGCACCA
  GGAGGTGCACGTGCACGAGCGCCGCTTCCGTTGTGGAGACTGTGGGAAGCTTTACAAGACCATCGCTCAT
  GTGCGGGGCCACCGGCGTGTTCACTCAGACGAGAGGCCTTTCCCTTGTCCCCAGTGCGGCAAGCGTTACA
  AAACCAAGAATGCCCAGCAAGTACACTTCCGGACACATCTGGAAGAAAAGCCCCACGTGTGCCAGTTCTG
  CAGCCGAGGCTTCCGGGAGAAGGGCTCTCTGGTGCGGCATGTGAGGCACCACACAGGCGAGAAACCTTTC
  AAGTGCTACAAGTGTGGCCGTGGCTTCGCGGAGCATGGCACACTCAACCGGCACCTGCGCACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC071228
• Insert Size: 1605 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC071228, AAH71228
• RefSeq Size: 2551 bp
• RefSeq ORF: 1604 bp
• Locus ID: 13560
• Cytogenetics: 17 12.39 cM
• Gene Summary: This gene encodes a member of the GLI-Kruppel zinc finger family. The encoded protein is likely to be multi-functional, with both adenovirus E1A-regulated transcription factor and ubiquitin E3 ligase activities, including roles in cell cycle regulation and the ubiquitination of p53. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]