Camk2b (NM_001174053) Mouse Untagged Clone

CAT#: MC217688

Camk2b (untagged) - Mouse calcium/calmodulin-dependent protein kinase II, beta (Camk2b), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Camk2b
• Synonyms: CaMKII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217688 representing NM_001174053
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCACGGTGACCTGCACCCGTTTCACCGACGAGTACCAGCTATACGAGGATATTGGCAAGGGGG
  CTTTCTCTGTGGTCCGACGCTGTGTCAAGCTCTGTACCGGCCATGAGTATGCAGCCAAGATCATTAATAC
  CAAGAAGCTGTCGGCCAGAGATCACCAGAAACTGGAGAGAGAAGCTCGGATCTGCCGCCTGCTGAAGCAT
  TCCAACATTGTACGCCTCCATGACAGCATCTCTGAAGAGGGCTTCCACTACCTGGTCTTCGATCTGGTCA
  CTGGTGGGGAGCTCTTTGAAGACATCGTGGCAAGAGAGTACTACAGTGAAGCTGATGCCAGTCACTGCAT
  CCAGCAGATCCTGGAAGCTGTTCTCCATTGTCACCAAATGGGGGTCGTCCACAGAGACCTCAAGCCTGAG
  AACCTGCTTCTGGCCAGCAAATGCAAAGGCGCCGCAGTGAAGCTGGCAGACTTCGGCCTGGCCATCGAGG
  TTCAGGGAGACCAGCAGGCATGGTTTGGATTTGCGGGAACGCCAGGCTACCTGTCTCCCGAGGTCCTTCG
  GAAGGAGGCCTACGGCAAACCTGTGGACATCTGGGCATGTGGGGTGATCCTGTATATCCTGCTGGTGGGC
  TACCCACCTTTCTGGGATGAGGACCAACACAAGCTGTACCAGCAGATCAAGGCTGGGGCGTATGATTTCC
  CATCCCCTGAGTGGGACACCGTTACTCCTGAAGCCAAAAACCTCATCAACCAGATGCTGACCATCAACCC
  TGCCAAGCGCATCACGGCCCATGAGGCCCTGAAGCACCCATGGGTCTGCCAACGTTCCACCGTGGCCTCT
  ATGATGCACAGACAGGAGACTGTGGAATGTCTGAAGAAGTTCAATGCAAGGAGGAAGCTCAAGGGAGCCA
  TCCTCACCACTATGCTGGCCACACGGAATTTCTCAGCCAAGAGTTTACTCAACAAGAAAGCAGATGGAGT
  CAAGCCCCAGACAAACAGCACCAAAAACAGCTCGGCCATCACCAGCCCCAAAGGATCTCTCCCTCCTGCT
  GCCCTGGAATCTTCCGACAGCACCAACACAACCATAGAGGATGAAGATGCCAAAGCCCCCAGGATCTCTG
  ACATCCTCAACTCAGTGAGGCGGGGCTCAGGGACCCCAGAAGCTGAGGGCCTCCCACCAGTGGGCCCCCC
  ACCCTGCCCATCTCCGACTCTCCCTGGCCCCTTGCCCACCCCATCCCGGAAGCAGGAAATCATCAAGACC
  ACAGAGCAGCTCATTGAGGCCGTCAACAATGGGGACTTTGAGGCCTATGCGAAAATCTGTGACCCAGGCC
  TGACCTCATTTGAGCCTGAAGCTCTGGGCAACCTGGTCGAAGGGATGGATTTCCACAGATTCTACTTTGA
  GAACCTGCTGGCCAAGAACAGCAAGCCGATCCACACCACCATCCTGAACCCGCACGTGCACGTCATTGGC
  GAGGATGCGGCATGCATCGCCTACATCCGCCTCACACAGTACATCGATGGCCAGGGCAGACCCCGTACCA
  GCCAGTCCGAAGAGACCCGTGTGTGGCACCGCCGCGACGGCAAGTGGCAGAATGTACATTTCCACTGCTC
  GGGCGCTCCAGTGGCCCCGCTGCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001174053
• Insert Size: 1638 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001174053.1, NP_001167524.1
• RefSeq Size: 4034 bp
• RefSeq ORF: 1638 bp
• Locus ID: 12323
• Cytogenetics: 11 3.89 cM
• Gene Summary: Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca(2+) transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Also regulates the migration of developing neurons (PubMed:29100089). Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2 (PubMed:21752990).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and it encodes the longest protein (isoform 1).