Actg1 (BC003337) Mouse Untagged Clone

CAT#: MC217684

Actg1 (untagged) - Mouse actin, gamma, cytoplasmic 1 (cDNA clone MGC:6223 IMAGE:3257312), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Actg1
• Synonyms: MGC28612, E51, Actg
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC003337
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAAGAAATCGCCGCACTCGTCATTGACAATGGCTCCGGCATGTGCAAAGCCGGCTTTGCTGGCG
  ACGACGCCCCCAGGGCCGTGTTCCCTTCCATCGTAGGGCGCCCCCGACACCAGGGCGTCATGGTGGGCAT
  GGGCCAGAAAGACTCATACGTGGGTGACGAGGCCCAGAGCAAGAGGGGTATCCTGACCCTGAAGTACCCT
  ATCGAACACGGCATTGTCACTAACTGGGACGACATGGAGAAGATCTGGCACCACACCTTCTACAATGAGC
  TGCGTGTGGCTCCTGAGGAGCACCCGGTGCTTCTGACCGAGGCCCCCCTGAACCCCAAAGCTAACAGAGA
  GAAGATGACGCAGATAATGTTTGAAACCTTCAATACCCCAGCCATGTACGTGGCCATTCAGGCGGTGCTG
  TCCTTGTATGCATCTGGGCGCACCACTGGCATTGTCATGGACTCTGGTGACGGGGTCACACACACAGTGC
  CCATCTATGAGGGCTACGCCCTTCCCCACGCCATCTTGCGTCTGGACCTGGCTGGCCGGGACCTGACAGA
  CTACCTCATGAAGATCCTGACTGAACGGGGCTACAGCTTTACCACCACTGCTGAGAGGGAAATTGTTCGT
  GACATAAAGGAGAAGCTGTGCTATGTTGCCCTGGATTTTGAGCAAGAAATGGCTACTGCTGCATCATCTT
  CCTCCTTGGAGAAGAGTTACGAGCTGCCCGACGGGCAGGTGATCACCATTGGCAATGAGCGGTTCCGGTG
  TCCGGAGGCACTCTTCCAGCCTTCCTTCCTGGGCATGGAGTCCTGTGGTATCCATGAGACCACTTTCAAC
  TCCATCATGAAGTGTGATGTGGATATCCGCAAAGACCTGTATGCCAATACAGTGCTGTCTGGTGGTACCA
  CCATGTACCCAGGCATTGCTGACAGGATGCAGAAGGAGATCACAGCCCTAGCACCTAGCACGATGAAGAT
  TAAGATCATTGCTCCCCCTGAGCGCAAGTACTCAGTCTGGATCGGTGGCTCCATTCTGGCCTCACTGTCC
  ACCTTCCAGCAGATGTGGATCAGCAAGCAGGAGTATGATGAGTCAGGCCCCTCCATCGTCCACCGCAAAT
  GCTTCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC003337
• Insert Size: 1128 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC003337, AAH03337
• RefSeq Size: 1903 bp
• RefSeq ORF: 1127 bp
• Locus ID: 11465
• Cytogenetics: 11 E2
• Gene Summary: Actins are highly conserved proteins that are involved in various types of cell motility and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta, and gamma, have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in non-muscle cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]