Arid5a (NM_145996) Mouse Untagged Clone

CAT#: MC217640

Arid5a (untagged) - Mouse AT rich interactive domain 5A (MRF1-like) (Arid5a), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arid5a
• Synonyms: D430024K22Rik; Mrf1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217640 representing NM_145996
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCACCTCCGGCCAAAGGGAACACAGAGCAGTCAGAAGAAGGTGACCTCCCGCAGCTTCCTGTAT
  CCCCCAAGCCAGATGATGAGCAGAGCAGGAGCCAGAGCCCCACCCAGCTCCAGGTGACAGGCCGCCGCCT
  CTGGAAGAACGTGTATGATGAACTTGGCGGTAGCCCAGGCAGCACCAGTGCGGCCACATGCACACGCCGC
  CACTATGAGAGGCTGGTCCTCCCATATGTGCGGCATCTGAAGGGGGAGGACGACAAGCCACTGCCTCCTA
  CCAAGCCCAGGAAGCAATACAAGATGGCCAAGGAGCTGAGGGGAGACGATGGGACCACTGAGAAGCTGAA
  GAAGGCCAAGGACTCAGAGGAGAGGCGGGTGGAGCAGACCACGCCAGGAAAGACCAAATCAGATGCCACT
  GGCCAGACACAGCTTCCCTGCCAGGGATCCTCGAGGGACAGCACAGAACAGCTGGGCCCAGTATCTGGAC
  CCTCTCCACCACTCACGGGTGCTAGTAGCTGCCCTGAGGCCTACAAGCGGCTCTTGTCAAGCTTTTACTG
  CAAAGGGGCGCATGGCATCATGTCACCACTGGCCAAAAAGAAACTCCTGGCCCAGGTCAGCAAGGCAGAG
  GCCTTGCAGTGCCAAGAAGAGGGCTGTCGCCATGGAGCAAGGAGCCCCAACAAGGACATTCAAGACAGTC
  CCCAGAACCTAAGAGGGCCGGCTGAGAACTCTGAACACCAGCTAACCCCCCGGGAAGGATTGCAGGCCCC
  TGGTGGGAGCACCAGGATGGAGGCCCAAGTGGGCCCCTGCCCTACAGCCCCCATGTTCTCAGGCTGTTTT
  CATGCGTACCCCACCGAGGTGCTGAAACCTGTCAGCCAGCACCCTAGGGACTTCTTCTCCGGCCTTAAAG
  ACAGGGTGCTGTTGGGACCACCTGGTAAAGAAGAAGGTCCGACAACCAAAGAGTCCCATCTGGTGTGGGG
  TGGGGATGCCAACCACCCCTCTGCATTCCATAAAGGCAGCACAAGAAAAAGAAGTTTCTACCCCAAACCC
  AAAGCCTGCTGGGTGTCTCCCATGGCCAAGGTCCCTACTGAGAGGCCTGGAGCCCCATCCCCTCATCCCA
  GTAGCCCAGGTCTTGGCAGTAAGCGCGGCTTGGAAGAAGAGGGATTCGCTCATGGTGGCAAGAAACTGAG
  GGCAGTGTCTCCCTTTCTGAAGGAGGTGGATTCCAAGGAGACTGGGGGCAAGCCTGCAGCCCCTGGCTTG
  GCTGTATCCTGTCTACTGGGCCCAACCCCGGGGCCCACTCCTCCAGAGGCCTACAGGGGCACCATGCTGC
  GGTGTCCTCTAAACTTCACCGGTAGCGCAGACCCTCTGAAGGGCCAGGCCTCACTCCCCTTCAGCCCCCT
  GGTCATCCCTGCTTTCCCAGCCCACCTTCTGGCTACAACAGGCTCCTCACCTATGGCTGCCAGCCTGATG
  CATTTCCCTCCCACGCCCTATGACGCTGTCCTACGCAACAGACTGGGTCCAGCTTCGTCTGCCTGGCACA
  TGCCACCCGTCACAACCTATGCGGCACCTCACTTCTTCCACCTCAACACCAAACTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145996
• Insert Size: 1599 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145996.4, NP_666108.2
• RefSeq Size: 5525 bp
• RefSeq ORF: 1599 bp
• Locus ID: 214855
• UniProt ID: Q3U108
• Cytogenetics: 1 B
• Gene Summary: DNA-binding protein that may regulate transcription and act as a repressor by binding to AT-rich stretches in the promoter region of target genes (By similarity). May positively regulate chondrocyte-specific transcription such as of COL2A1 in collaboration with SOX9 and positively regulate histone H3 acetylation at chondrocyte-specific genes. May stimulate early-stage chondrocyte differentiation and inhibit later stage differention (PubMed:21346191). Can repress ESR1-mediated transcriptional activation; proposed to act as corepressor for selective nuclear hormone receptors (By similarity). As RNA-binding protein involved in the regulation of inflammatory response by stabilizing selective inflammation-related mRNAs, such as IL6, STAT3 and TBX21. Binds to stem loop structures located in the 3' UTRs of IL6, STAT3 and TBX21 mRNAs; at least for STAT3 prevents binding of ZC3H12A to the mRNA stem loop structure thus inhibiting its degradation activity. Contributes to elevated IL6 levels possibly implicated in autoimmunity processes. IL6-dependent stabilization of STAT3 mRNA may promote differentiation of naive CD4+ T-cells into T-helper Th17 cells (PubMed:23676272, PubMed:27022145). In CD4+ T-cells may also inhibit RORC-induced Th17 cell differentiation independently of IL6 signaling (PubMed:24782182). Stabilization of TBX21 mRNA contributes to elevated interferon-gamma secretion in Th1 cells possibly implicated in the establishment of septic shock (PubMed:27671645).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks two alternate in-frame exons in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1.