Lpcat1 (NM_145376) Mouse Untagged Clone

CAT#: MC217637

Lpcat1 (untagged) - Mouse lysophosphatidylcholine acyltransferase 1 (Lpcat1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lpcat1
• Synonyms: 2900035H07Rik; Aytl2; BB137372; BC005662; C87117; LPCAT; rd11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217637 representing NM_145376
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCTGCGGGGCCGCGGGCCGCGGGCCGCCCCCTCCTCCAGCTCTGGGGCAGGCGACGCGCGGCGCC
  TAGCGCCCCCGGGGCGGAACCCCTTCGTGCACGAGCTGCGACTGAGCGCCCTGCAGAAGGCCCAGGTGGC
  TTTCATGACGTTGACGCTGTTCCCCATCCGGCTCCTGTTCGCTGCTTTCATGATGCTGCTGGCCTGGCCC
  TTTGCACTCCTGGCTTCCCTGGGACCTCCTGATAAGGAGCCAGAGCAGCCCCTGGCCTTATGGAGGAAGG
  TCGTGGACTTCCTGCTCAAGGCCATCATGCGCACCATGTGGTTTGCTGGCGGCTTCCACCGTGTAGCTGT
  GAAGGGGCGGCAGGCCCTGCCTACTGAGGCTGCTATCCTCACCTTGGCTCCACATTCCTCCTACTTTGAT
  GCCATCCCGGTCACCATGACCATGTCCTCCATTGTGATGAAGGCAGAGAGCAGAGACATCCCAATCTGGG
  GAACTCTGATAAGATACATCAGGCCAGTGTTCGTGTCCCGCTCTGACCAGGACTCGCGAAGGAAGACAGT
  GGAGGAGATCAAGCGACGGGCACAGTCGAATGGAAAGTGGCCTCAGATAATGATTTTTCCAGAAGGAACT
  TGTACAAATAGGACCTGCCTCATTACCTTCAAACCTGGTGCGTTCATTCCTGGAGTTCCTGTCCAGCCCG
  TGGTGCTACGCTACCCAAACAAATTGGACACCATCACATGGACGTGGCAAGGACCTGGAGCGTTGAAAAT
  CCTGTGGCTCACTCTGTGCCAGTTTCAAAACCAAGTGGAAATTGAATTTCTGCCTGTGTATTGCCCTTCT
  GAAGAGGAGAAGAGGAATCCTGCCCTGTATGCCAGCAATGTGAGGCGTGTCATGGCCAAGGCCCTGGGTG
  TCTCAGTAACCGACTATACATTTGAGGACTGCCAGCTGGCTCTGGCAGAAGGACAACTTCGCTTGCCTGC
  TGACACCTGCCTGCTAGAGTTTGCCAGGCTTGTGAGGGGCCTCGGACTAAAACCAGAAAATCTTGAAAAA
  GATCTAGACAAATATTCAGAAAGTGCGAGGATGAAGAGAGGAGAGAAAATCCGCCTTCCAGAGTTTGCGG
  CCTACTTGGAAGTTCCTGTCTCAGATGCACTGGAAGACATGTTCTCGCTTTTTGACGAGAGTGGTGGTGG
  TGAGATTGACCTTCGTGAATATGTGGTCGCCTTGTCTGTGGTGTGCAGGCCATCCCAGACCTTAGCCACC
  ATCCAGCTGGCATTCAAGATGTACGGATCTCCTGAGGATGGCAGCATAGATGAGGCCAACCTGTCCTGCA
  TCCTCAAGACTGCACTGGGTGTATCAGAATTAACAGTGACCGACTTGTTCCAGGCTATTGACCAAGAGGA
  CAAGGGAAGAATCACCTTTGATGACTTCTGCGGGTTTGCGGAAATGTACCCCGACTACGCAGAGGACTAC
  TTGTACCCTGATCAGACACATTTCGACAGCTGTGCACAGACACCCCCAGCACCAACTCCCAATGGCTTCT
  GCATTGACTTCAGCCCTGAGAACTCAGACTTTGGGAGAAAGAATTCTTGTAAGAAAGCGGACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145376
• Insert Size: 1605 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145376.5, NP_663351.3
• RefSeq Size: 1605 bp
• RefSeq ORF: 1605 bp
• Locus ID: 210992
• UniProt ID: Q3TFD2
• Cytogenetics: 13 C1
• Gene Summary: Possesses both acyltransferase and acetyltransferase activities (PubMed:16704971). Activity is calcium-independent (PubMed:18285344). Mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC) (PubMed:16704971, PubMed:18156367). Displays a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively (PubMed:16704971, PubMed:18285344). May synthesize phosphatidylcholine in pulmonary surfactant, thereby playing a pivotal role in respiratory physiology (PubMed:16704971). Involved in the regulation of lipid droplet number and size (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.