Arfgap2 (NM_001166024) Mouse Untagged Clone

CAT#: MC217620

Arfgap2 (untagged) - Mouse ADP-ribosylation factor GTPase activating protein 2 (Arfgap2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arfgap2
• Synonyms: 2310032E02Rik; Zfp28; Zfp289
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217620 representing NM_001166024
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGAGCCCGAGCAAGACCGAAATCCAGACTATTTTTAAGAGACTTCGCGCAATTCCCACCAACA
  AGGCCTGTTTCGATTGTGGCGCCAAGAGTCCTAGTTGGGCCAGCATCACTTACGGTGTATTTTTGTGTAT
  TGACTGCTCCGGAGTGCATCGCTCCTTGGGTGTCCATCTCAGCTTTATCAGGTCCACAGAGTTGGATTCG
  AACTGGAGCTGGTTGCAGCTGAGGTGTATGCAGGTCGGCGGGAATGCCAATGCGACTGCTTTTTTCCGCC
  AACACGGATGTATGGCCAATGATGCTAACACCAAATATACGAGCCGAGCTGCCCAGATGTACCGAGAGAA
  GATCCGGCAGTTAGGGAGTGCAGCTCTGACTAGGCATGGTACTGATCTTTGGATAGACAGTATGAACAGT
  GCTCCAAGTCACTCTCCAGAGAAGAAAGACTCTGATTTCTTCACAGAACATACTCAGGCCCCTGCTTGGG
  ATACAGCAGCCACTGATCCTTCAGGGACTCAGCAACCAGCCCTACCTTCAGAGAGCAGTAGCCTGGCACA
  ACCTGAACAAGGCCCTAACACTGATCTGCTCGGAACCTCACCCCAAGCCTCTCTGGAGTCCATGTATCTG
  TCAGCTAAAGGGCCCTCTTGTACCAGAGAACTGAAAAGCTCCATTATTGGCAAGAAGAAGCCAGCAGCCG
  CTAAGAAAGGGCTGGGTGCCAAGAAAGGCCTGGGAGCTCAGAAGGTGAGCAACCAGAGCTTTACTGAGAT
  TGAGCGGCAGGCCCAGGTGGCAGAGAAGCTCCGAGAGCAGCAGGCAGCTGATGCCAAGAAGCAGGCAGAA
  GAGTCCATGGTTGCCTCCATGCGTCTGGCCTACCAGGAGCTCCAGATTGATCGCAAGAAGGAGGAGAAAA
  AGCTCCAGAATCTTGAAGGAAAGAAGCGAGAGCAGGCTGAAAGGCTGGGCATGGGCTTGGTGTCCCGAAG
  CTCCATCTCCCATTCTGTGCTGTCTGAGATGCAGATGATTGAGCAGGAAACCCCGCTGAGTGCGAAATCT
  TCCCGTTCACAGCTTGACTTGTTTGATGATGTTGGAACATTTGCATCTGGACCCCCAAAGTACAAGGACA
  ACCCCTTTTCCTTGGGAGAAACTTTTGGTTCCCGCTGGGATTCAGATGCAGCCTGGGGTATGGACAGGGT
  AGAAGAGAAGGAACCTGAAGTTACCATCTCCAGCATCCGGCCTATTTCAGAAAGAACTGCGAGTCGGAGG
  GAAGTGGAGACTCGGAGCTCAGGCCTTGAGTCCAGTGAAGCACGGCAGAAGTTTGCAGGAGCCAAAGCCA
  TCTCGTCCGACATGTTCTTTGGTCGTGAGGTGGATTCTGAGTATGAAGCTAGGTCTCGGTTACAGCAGCT
  TTCGGGCAGCAGTGCCATCAGCTCTTCAGACCTTTTTGGAAACATGGATGGAGCTCATGGAGGAGGAACT
  GTATCTCTGGGGAATGTACTTCCTACAGCTGACATTGCCCAGTTTAAGCAGGGTGTCAAGTCTGTGGCTG
  GCAAGATGGCTGTGCTGGCCAATGGTGTGATGAATTCCTTACAGGATCGCTACGGTTCCTACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001166024
• Insert Size: 1605 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166024.1, NP_001159496.1
• RefSeq Size: 3409 bp
• RefSeq ORF: 1605 bp
• Locus ID: 77038
• UniProt ID: Q99K28
• Cytogenetics: 2 E1
• Gene Summary: This gene encodes a zinc-finger-containing GTPase-activating protein for ADP ribosylation factor 1 (ARF1), a small GTPase that plays a role in coatomer-mediated vesicular trafficking. This gene product stimulates the hydrolysis of ARF1-bound GTP, which may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes. It may regulate the retrograde transport from the Golgi complex to the endoplasmic reticulum. Expression of this gene has been shown to be controlled by inhibitor of DNA binding 1 (Id1). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene was identified on chromosome 6. [provided by RefSeq, Oct 2009]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.