Fam227b (NM_029455) Mouse Untagged Clone

CAT#: MC217619

Fam227b (untagged) - Mouse RIKEN cDNA 4930525F21 gene (4930525F21Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fam227b
• Synonyms: 4930525F21Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217619 representing NM_029455
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTGCCTGTCCCTCAGAAATTACCGGATCCCCCAAGGAGCTTTGATGAATTCTTAAAGTCTCAAA
  ACTGGGATTATTGGCCCAGGGATGTTCATTTTAGAGATAGTGATATATGGGAAGATACTCTGAAAAAACT
  AGAAGAGGCTATTTCATATACTTCTATTTATTCTTATCTGTGGACCAACGTGCCTCGGCTGTATGATATC
  GTCGACAGCTTGGAGTCAAAATTAAAGGAAAGCTCACATCTTTTGCAACACCATGCCTCCAGATTATTTG
  AAAGTGACAAAATGATTTCCAAGAAAAGGTCATATACAAACCTGGAACGCTACAAAGCATTTGTAAAAGA
  ACACTATAGGCCAAAGAAAATCGCGCTTTCAGATCGAATGGAGACAGAGAAGAATATAGAGGGTTGCACC
  TTTTTATTCAACCAAAATGAAGTGACACAATTACCCAGACATTTGGATGCTAAACAAATTTATCTTTATG
  TTTTAAAAACACATAACTTTGAGGAAAAGGTATTTAAAGTCTGGAAAACCCATGTTCTCTCAGATTGCTC
  CATTGCTCTTTTGCATGACTCCTTTTGGTGGTGGTTTCTACATAAATTCAAGCCTGACAAAAGAGATGAA
  GATTTCTTGTTTGATAGAATTGCAGAAAGTTATGTCACACTTTTCATAAAAATACCTCTAAGGAGAAAGG
  ATGCATTCTTTAAGATGTATCCTGACTGCTTGACTCAAGCTGTCTACACAACCTTCCAGGAATCATTTCC
  AGAATCCTGCAGCCTCTTCAATGATAAATTCAAAGAAGACCTAGGAAACACGATTTTTCTTTGGTTGTCA
  GGTCTAAAGCCTGAAACTGGCTTTTGGACCCACTGGAAACTGCAAGACCTCTGCACAACCACCATTCATG
  GCAGCAGGCGTGTACCTGTGAAGTTGAGAAGGAGCGTCATTCCCAGCCAGGAGCACATACCAGGCATTAG
  AGACTTGAAAATCGAGGATATTCTAAAGAACCCAAGAGCGTACGCAATGCCTAAACTCATGAAAGAGTCC
  GTAGCATCAAAAGCAGCAACCAAGCCTTCCCACTATAGGAGCTTGGGTCCAGAGTTTCACAAAGTTCTCT
  TTGACTTTGGAGGCCAGAGCCCACTGATTCTGTACTATCTTAAGATGCACGAGCTAGGAGGTATTTCCGT
  CACTCACAATCCGAAGGGAACCAAGTTCACCAAGATACTCCGGGAGCCATCACCTGCTCCAACATACTGC
  GACATCATCAGGGATGCCAAAAGAAAATTTGCAGATAACAAGAAGGATTTTAAGAGAGTAAAGCAAAGAA
  TCAAAGATGATATCAAGTTCCTGAGAGAGCAACAGGAACTAATTGACAAGGAGCTTGACCGGATCCAAGC
  AAAGGCTTCCAAAAATCTCCAAGAAGTCAAAAATGAATTTGAGAATTTTCTACATAAACAACGTGTTGAG
  GCAAAACTCAAGGAGGAATATGGAGGATCAACATCAGCATCAGAGTCGCCACAGTCGATGCAGTCACCAC
  AATCATCATCGTCATTTCCTACTATATCAGAAGACTTCAACAATGTGGAAGAGGGCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029455
• Insert Size: 1599 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029455.3, NP_083731.2
• RefSeq Size: 1858 bp
• RefSeq ORF: 1599 bp
• Locus ID: 75823
• UniProt ID: Q9D518
• Cytogenetics: 2 F1