Arhgef3 (NM_027871) Mouse Untagged Clone

CAT#: MC217612

Arhgef3 (untagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 3 (Arhgef3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arhgef3
• Synonyms: 1200004I24Rik; 9830169H03Rik; C76747; Xpln
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217612 representing NM_027871
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGTCGGAGCGCCCCATGGTGTGGTGCTGTTTTTTTCTCCGTGCACAGCGGAAACGAAAACAGAGTT
  CCCAGGATGAAGATGCTGTCAGCCTGTGCAGTCTTGACATAAGTGAACCTAGTAACAAAAGGGTCAAACC
  CCTTTCCAGAGTCACATCGCTAGCAAACCTCATTCCACCTGTGAAGACCACACCATTAAAGCGCTTCAGC
  CAGACCTTGCAGCGGTCCATCAGCTTCCGCAGCGAGAGCCGCCCCGACATCCTTGCCCCCCGAGCTTGGT
  CCAGAAATGCCACCTCCTCAAGCACAAAGCGGAGAGACAGCAAACTGTGGAGTGAGACCTTCGATGTGTG
  TGTCAATCAGGTGCTCACGGCCAAGGAGATCAAGAGACAGGAGGCGATCTTTGAACTTTCTCAAGGTGAA
  GAAGACTTGATAGAGGACTTGAAATTAGCAAAAAAGGCCTACCACGACCCCATGCTGAAACTCTCCATAA
  TGACGGAACAGGAACTGAATCAGATTTTTGGGACCCTGGACTCTCTAATTCCTCTCCATGAAGAGCTCCT
  CAGTCAGCTTCGAGATGTTCGGAAACCCGATGGCTCGACTGAGCACGTTGGTCCCATCCTCGTGGGCTGG
  CTGCCCTGTCTCAGCTCCTACGACAGCTACTGCAGCAATCAAGTCGCCGCCAAAGCCCTGCTGGACCACA
  AAAAGCAGGACCACAGAGTGCAGGACTTCCTCCAGCGCTGTTTAGAATCACCCTTCAGTCGCAAACTCGA
  TCTCTGGAATTTCCTTGATATTCCCCGGAGCCGGCTAGTGAAGTATCCTCTGCTCCTTCGAGAAATCCTG
  AGGCATACACCAAATGATAACCCAGACCAGCAGCACCTGGAAGAAGCTATAAACATCATCCAGGGAATTG
  TGGCAGAAATCAACACCAAGACTGGGGAATCGGAATGCCGCTATTACAAAGAGCGGCTCCTTTACCTGGA
  AGAAGGCCAGAAAGATTCTCTGATTGACAGCTCAAGGGTCCTGTGTTGTCATGGAGAGCTGAAAAATAAC
  CGGGGTGTGAAACTCCATGTTTTCCTGTTCCAAGAAGTGCTGGTGATCACCCGAGCTGTCACTCACAACG
  AGCAGCTCTGCTATCAGTTGTACCGACAGCCCATCCCTGTGAAGGACCTCACGCTGGAAGACCTGCAGGA
  TGGAGAAGTGCGGCTGGGTGGCTCCCTGCGCGGGGCCTTCAGCAACAACGAGAGAGTTAAAAACTTCTTC
  AGAGTCAGTTTCAAAAATGGATCCCAAAGTCAGACCCACTCACTACAAGCCAACGACACCTTCAACAAAC
  AGCAGTGGCTCAACTGTATCCGTCAGGCAAAAGAAACTGTTCTGTCTGCTGCCGGCCAGGCTGGCTTGCT
  TGACTCTGAGGGACTGGTCCAGGGTCCCGGCACTGAGAACAGGGAGCCACAGGGAGAAACAAAACTTGAG
  CAGATGGACCAATCGGACAGCGAGTCGGACTGCAGTATGGACACCAGTGAGGTCAGCCTTGAGTGTGAGC
  GGATGGAACAGACAGACGCTTCCTGTGCAAACAGCAGGCCCGAGGAGAGTGTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027871
• Insert Size: 1596 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027871.2, NP_082147.1
• RefSeq Size: 3637 bp
• RefSeq ORF: 1596 bp
• Locus ID: 71704
• UniProt ID: Q91X46
• Cytogenetics: 14 16.09 cM
• Gene Summary: Acts as guanine nucleotide exchange factor (GEF) for RhoA and RhoB GTPases.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has multiple differences at its 5' end, compared to variant 1. These differences result in a distinct 5' UTR and cause translation initiation at a downstream start codon, compared to variant 1. The encoded protein (isoform 2) is shorter and has a distinct N-terminus, compared to isoform 1.