Gp2 (NM_025989) Mouse Untagged Clone

CAT#: MC217606

Gp2 (untagged) - Mouse glycoprotein 2 (zymogen granule membrane) (Gp2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gp2
• Synonyms: 2310037I18Rik; AV060639
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217606 representing NM_025989
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAAGGATGGTGGGTTGTGACCTGCTGTGGCTGGCTGCAGCCTCCTGCGTTCTGACACTGGTATCTC
  CATCTACAATACATCAAGGTTATGGGAGGCCCAGGAACAGCTCCAATCTGGACTTGGACTGTGGATCCCC
  TGACTCTCCAAGTTCTGGAATCTGCTTTGATCCCTGCCAGAATCACACGGTCCTGAATGATCCTACCAGA
  AGCACAGAGAACAACGATTCATCAGTTGCATGGTGTGATGATAACCTGCACGGCTGGTACCGCTTTGTGG
  GTGATGGAGGAGTGAAGATGCCAGAGACCTGTGTGAGTGTGTTCCGGTGCCACACCTCTGCCCCCATGTG
  GCTGAGTGGATCTCACCCCATCCTTGGAGATGGCATTGTCAGCCACACAGCCTGTGCCAACTGGAATGAA
  AATTGTTGCTTCTGGCGTTCTGAGGTCCAGGTGAAGGCCTGTTCTGAAGAACTAGGAGAATATCACGTGT
  ACAAGTTACAGGGTACCCCTGAATGCAGTCTGAGATACTGCACAGACCCCTCCACTGCACCCAAGAATTG
  TGAGATTACTTGTCGTCCTGAGGAGGAATGTGTATTCCAAAATAACAACTGGTCCTGTGTCTGTAGGCAG
  GACCTCCATGTTTCTGACTCTCAGAGTTTGCAGCCTCTTCTGGACTGTGGAGATAATGAGATCAAGGTGA
  AGTTGGACAAGTGTTTGCTGGGAGGTATGGGTTTCAAGGAGGAGATCATTGCCTACCTCAATGACCGGAA
  CTGCAATGGTACCATGCAAGATGAGCCCAACAACTGGGTGTCCATGACCAGCCCTGTTGTGGCTAATTAC
  TGTGGGAACATCCTGGAGAAAAATGGGACCCATGCCATCTACAGAAACACCCTTTCCTTGGCCACTGACT
  TTATTATCAGGGACTTTCGTGTCAATGTCAACTTCCAGTGTGCCTACCCACTGGACATGAGCGTCAGCCT
  CGAAACTGCTCTCCAGCCCATTGTAAGCTCCCTGACTGTTGATGTGGACGGAGCAGGAGAGTTCAATGTC
  AAGATGGCCCTCTTCCAAGACCAGAGCTACACAAACCCTTATGAAGGAGCCGAAGTGTTGCTTCCAGTGG
  AGTCTATACTCTATGTGGGTGTCCTCTTAAATAGAGGGGATACCTCCAGGTTCAAACTGTTGCTGACCAA
  CTGCTATGCCACCCCTTCAGAAGACAGGCATGACCCTGTGAAGTACTTCATCATCAAGAACAGATGCCCA
  AACCAACGTGATTCCACCATCAATGTGCGGGAGAATGGTGTGTCCTCAGAAAGCCGATTTTCAGTGCAGA
  TGTTCATGTTTGCTGGAAATTATGACTTAGTTTTCCTGCATTGTGAGGTTTACCTGTGTGACTCCACTAC
  TGAACAGTGTCAACCATCTTGCTCTACAAATCGACTTCGCAGTAGTAGACCAGCCATTGACTATAATCGG
  GTTCTGGATTTAGGACCCATCACTAAAAGAAGTGCTCAGAGTTCTGCTACCTCGAAGGGGACTCCCCACA
  CTACAGGGTTCTTGTTGGCCTGGCCCATGTTCTTCCTGCCTGTCTTCCTGGCTCTACTGTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_025989
• Insert Size: 1605 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025989.3, NP_080265.2
• RefSeq Size: 1978 bp
• RefSeq ORF: 1605 bp
• Locus ID: 67133
• UniProt ID: Q9D733
• Cytogenetics: 7 63.88 cM
• Gene Summary: Functions as an intestinal M-cells transcytotic receptor specific of type-I-piliated bacteria that participates to the mucosal immune response toward these bacteria. At the apical membrane of M-cells binds fimH, a protein of the bacteria type I pilus tip. Internalizes bound bacteria, like E.coli and S.typhimurium, from the lumen of the intestine and delivers them, through M-cells, to the underlying organized lymphoid follicles where they are captured by antigen-presenting dendritic cells to ellicit a mucosal immune response.[UniProtKB/Swiss-Prot Function]