Zbtb18 (NM_001012330) Mouse Untagged Clone

CAT#: MC217589

Zbtb18 (untagged) - Mouse zinc finger protein 238 (Zfp238), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zbtb18
• Synonyms: RP58; zfp-238; Zfp238; Znf238
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217589 representing NM_001012330
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTCCTAAAGGTTATGAAGACAGTATGGAGTTTCCAGACCACAGCAGACATTTGCTGCAGTGTCTGA
  GCGAGCAGAGGCACCAGGGCTTTCTTTGTGACTGCACTGTTCTGGTGGGAGATGCCCAGTTCCGGGCACA
  CCGAGCTGTTCTGGCTTCATGCAGCATGTATTTCCACCTCTTTTACAAGGACCAGCTGGACAAAAGAGAC
  ATTGTTCATCTGAACAGCGACATTGTGACAGCCCCCGCTTTCGCTCTCCTGCTTGAATTCATGTACGAAG
  GGAAACTCCAGTTCAAAGACTTGCCCATTGAGGACGTGCTAGCAGCTGCCAGTTATCTCCACATGTATGA
  CATTGTCAAAGTCTGCAAAAAGAAGCTGAAAGAGAAAGCTACCACAGAGGCGGACAGCACCAAAAAAGAA
  GAAGATGCTTCAAGTTGTTCGGATAAAGTCGAGAGCCTCTCAGACGGCAGCAGCCACATGGCAGGTGACC
  TGCCCAGTGATGAAGATGAAGGCGAAGATGACAAATTGAACATTCTACCCAGCAAAAGGGACTTGGCAGC
  TGAGCCTGGGAACATGTGGATGCGATTGCCCTCAGACTCAGCAGGCATCCCCCAGGCTGGCGGAGAGGCC
  GAGCCACACGCCACAGCAGCTGGAAAAACAGTAGCCAGCCCCTGCAGCTCAACAGAGTCTTTGTCCCAGA
  GGTCTGTCACCTCCGTGAGGGATTCGGCAGATGTTGACTGTGTGCTGGACCTGTCTGTCAAGTCCAGCCT
  TTCAGGAGTTGAAAATCTGAACAGCTCTTATTTCTCTTCACAGGACGTGCTGAGAAGCAACCTGGTGCAG
  GTGAAGGTGGAGAAAGAGGCGTCCTGTGATGAGAGTGATGTTGGCACTAATGACTATGACATGGAACATA
  GCACTGTGAAAGAGAGTGTGAGCACTAACAACAGGGTACAGTATGAGCCAGCCCACCTGGCTCCTCTGAG
  GGAGGACTCGGTCTTAAGGGAACTGGATCGGGAGGACAAAGCCAGCGATGATGAGATGATGACCCCAGAG
  AGCGAGCGGGTCCAGGTGGAAGGGGGCATGGAGAACAGTCTGCTTCCCTACGTCTCCAATATCCTGAGCC
  CCGCAGGCCAGATCTTCATGTGCCCCCTGTGCAACAAAGTCTTCCCCAGCCCCCACATCCTGCAGATCCA
  CCTGAGCACGCACTTCCGAGAGCAGGATGGCATCCGTAGCAAGCCTGCCGCCGATGTCAATGTGCCCACG
  TGCTCCCTGTGTGGGAAGACTTTCTCCTGCATGTACACCCTCAAGCGCCACGAGAGGACTCACTCGGGGG
  AGAAGCCCTACACGTGCACCCAATGCGGCAAGAGTTTCCAGTACTCGCACAACCTGAGCCGTCATGCCGT
  GGTGCACACCCGCGAGAAGCCCCATGCCTGCAAGTGGTGCGAGCGCAGGTTCACGCAGTCCGGAGACCTG
  TACAGACACATCCGCAAGTTCCACTGTGAGTTGGTGAACTCCTTGTCGGTCAAAAGTGAAGCGCTGAGCT
  TGCCCACTGTCAGAGACTGGACCTTAGAAGATAGCTCTCAAGAACTTTGGAAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001012330
• Insert Size: 1596 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012330.1, NP_001012330.1
• RefSeq Size: 3848 bp
• RefSeq ORF: 1596 bp
• Locus ID: 30928
• UniProt ID: Q9WUK6
• Cytogenetics: 1 H4
• Gene Summary: Transcriptional repressor that plays a role in various developmental processes such as myogenesis and brain development. Specifically binds the consensus DNA sequence 5'-[AC]ACATCTG[GT][AC]-3' which contains the E box core, and acts by recruiting chromatin remodeling multiprotein complexes. Plays a key role in myogenesis by directly repressing the expression of ID2 and ID3, 2 inhibitors of skeletal myogenesis. Also involved in controlling cell division of progenitor cells and regulating the survival of postmitotic cortical neurons. May also play a role in the organization of chromosomes in the nucleus.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the shorter transcript but encodes the longer isoform (1).