Kpna6 (NM_008468) Mouse Untagged Clone

CAT#: MC217568

Kpna6 (untagged) - Mouse karyopherin (importin) alpha 6 (Kpna6), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kpna6
• Synonyms: IPOA7; Kpna5; NPI-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217568 representing NM_008468
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACCATGGCAAGCCCAGGGAAGGACAATTATCGAATGAAGAGCTATAAGAACAATGCCTTAAACC
  CTGAGGAAATGAGACGAAGGAGGGAGGAAGAGGGCATTCAGCTTCGGAAACAGAAGCGGGAACAACAACT
  TTTTAAACGGAGAAATGTAGAGCTGATTAATGAAGAAGCTGCCATGTTTGATAGTCTGCTGATGGATTCC
  TACGTGAGCTCTACAACTGGGGAGAGCGTGATCACGAGAGAGATGGTAGAGATGCTCTTCTCGGACGACT
  CTGACTTGCAGTTAGCAACCACGCAGAAATTCCGGAAACTCCTGTCCAAAGAGCCGAGTCCTCCAATAGA
  TGAAGTTATTAACACTCCAGGAGTAGTTGATCGGTTTGTGGAGTTTCTGAAGAGAAATGAGAATTGTACA
  TTGCAGTTTGAAGCTGCCTGGGCTCTAACAAACATTGCCTCTGGAACGTCTCAGCAGACCAAAATTGTCA
  TTGAAGCAGGAGCTGTCCCCATTTTTATAGAGTTACTTAACTCAGACTTTGAAGATGTACAAGAACAGGC
  AGTGTGGGCGCTTGGAAACATCGCTGGAGATAGCTCGCTTTGCAGGGATTATGTCTTGAATTGCTCCATC
  CTTAACCCCTTGTTAACACTCCTTACTAAGTCCACACGACTGACAATGACACGGAATGCAGTCTGGGCCC
  TGTCAAACCTCTGCCGAGGCAAGAACCCTCCTCCAGAGTTTGCAAAGGTCTCTCCTTGTTTGCCTGTATT
  GTCCCGACTGCTCTTCAGCAGTGACTCAGACTTGCTGGCAGATGCTTGCTGGGCCCTCTCCTACCTGTCT
  GATGGCCCCAATGAGAAGATCCAGGCAGTCATAGACTCTGGAGTCTGCCGGAGATTGGTAGAGCTTCTGA
  TGCACAACGATTACAAAGTGGCGTCTCCTGCCTTGAGAGCTGTGGGTAATATTGTCACTGGGGATGACAT
  CCAGACCCAGGTCATTCTTAATTGTTCAGCCCTCCCTTGCCTCCTCCACCTACTGAGCAGCTCTAAGGAA
  TCAATCCGGAAGGAAGCTTGCTGGACCATTTCAAACATCACTGCTGGCAACAGGGCTCAAATACAGGCTG
  TCATAGATGCAAACATCTTCCCTGTATTGATTGAAATCCTTCAGAAAGCAGAGTTCCGTACAAGGAAAGA
  AGCAGCCTGGGCCATCACCAACGCCACATCAGGAGGAACTCCTGAGCAAATCAGGTACCTGGTATCACTG
  GGCTGCATTAAGCCCCTGTGTGACTTGCTGACTGTAATGGATTCAAAAATTGTGCAAGTGGCCCTCAATG
  GACTAGAGAACATTCTTCGGCTTGGAGAGCAAGAAAGCAAGCGTAGTGGCTCAGGGGTCAATCCCTACTG
  TGGCCTCATCGAGGAAGCCTATGGCTTGGATAAAATTGAGTTTCTCCAGAGCCACGAGAACCAGGAAATC
  TACCAGAAGGCCTTTGACCTCATTGAGCACTACTTTGGTGTAGAGGATGACGACAGCAGCCTGGCTCCTC
  AGGTGGATGAGACGCAACAGCAGTTCATCTTCCAGCAGCCTGAGGCCCCCATGGAGGGCTTCCAGCTATA
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008468
• Insert Size: 1611 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008468.4, NP_032494.3
• RefSeq Size: 5711 bp
• RefSeq ORF: 1611 bp
• Locus ID: 16650
• UniProt ID: O35345
• Cytogenetics: 4 D2.2
• Gene Summary: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.[UniProtKB/Swiss-Prot Function]