Hexb (NM_010422) Mouse Untagged Clone

CAT#: MC217566

Hexb (untagged) - Mouse hexosaminidase B (Hexb), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hexb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217566 representing NM_010422
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCAGTCCCCGCGTAGCGCCCCCGGGCTGCTGCTGCTGCAGGCGCTGGTGTCGCTAGTGTCGCTGG
  CCCTAGTGGCCCCGGCCCGACTGCAACCTGCGCTATGGCCCTTCCCGCGCTCGGTGCAGATGTTCCCGCG
  GCTGTTGTACATCTCCGCGGAGGACTTCAGCATCGACCACAGTCCCAATTCCACAGCGGGCCCTTCCTGC
  TCGCTGCTACAGGAGGCGTTTCGGCGATATTACAACTATGTTTTTGGTTTCTACAAGAGACATCATGGCC
  CTGCTAGATTTCGAGCTGAGCCACAGTTGCAGAAGCTCCTGGTCTCCATTACCCTCGAGTCAGAGTGCGA
  GTCCTTCCCTAGTCTGTCTTCAGATGAAACCTATTCTCTGCTTGTACAAGAACCAGTAGCCGTCCTCAAG
  GCCAACAGCGTTTGGGGAGCGTTACGAGGTTTAGAGACGTTTAGCCAGTTAGTTTACCAAGACTCTTTCG
  GGACTTTCACCATCAATGAATCCAGTATAGCTGATTCTCCAAGATTCCCTCATAGAGGAATTTTAATTGA
  TACATCTAGACACTTCCTGCCTGTGAAGACAATTTTAAAAACTCTGGATGCCATGGCTTTTAATAAGTTT
  AATGTTCTTCACTGGCACATAGTGGACGACCAGTCTTTCCCTTATCAGAGTACCACTTTTCCTGAGCTAA
  GCAATAAGGGAAGCTACTCTTTGTCTCATGTCTATACACCAAACGATGTCCGGATGGTGCTGGAGTACGC
  CCGGCTCCGAGGGATTCGAGTCATACCAGAATTTGATACCCCTGGCCATACACAGTCTTGGGGCAAAGGA
  CAGAAAAACCTTCTAACTCCATGTTACAATCAAAAAACTAAAACTCAAGTGTTTGGGCCTGTAGACCCAA
  CTGTAAACACAACGTATGCATTCTTTAACACATTTTTCAAAGAAATCAGCAGTGTGTTTCCAGATCAGTT
  CATCCACTTGGGAGGAGATGAAGTAGAATTTCAATGTTGGGCATCAAATCCAAACATCCAAGGTTTCATG
  AAGAGAAAGGGCTTTGGCAGCGATTTTAGAAGACTAGAATCCTTTTATATTAAAAAGATTTTGGAAATTA
  TTTCATCCTTAAAGAAGAACTCCATTGTTTGGCAAGAAGTTTTTGATGATAAGGTGGAGCTTCAGCCGGG
  CACAGTAGTCGAAGTGTGGAAGAGTGAGCATTATTCATATGAGCTAAAGCAAGTCACAGGCTCTGGCTTC
  CCTGCCATCCTTTCTGCTCCTTGGTACTTAGACCTGATCAGCTATGGGCAAGACTGGAAAAACTACTACA
  AAGTTGAGCCCCTTAATTTTGAAGGCTCTGAGAAGCAGAAACAACTTGTTATTGGTGGAGAAGCTTGCCT
  GTGGGGAGAATTTGTGGATGCAACTAACCTTACTCCAAGATTATGGCCTCGAGCAAGCGCTGTTGGTGAG
  AGACTCTGGAGCCCTAAAACTGTCACTGACCTAGAAAATGCCTACAAACGACTGGCCGTGCACCGCTGCA
  GAATGGTCAGCCGTGGAATAGCTGCACAACCTCTCTATACTGGATACTGTAACTATGAGAATAAAATATA
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010422
• Insert Size: 1611 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010422.2, NP_034552.1
• RefSeq Size: 1922 bp
• RefSeq ORF: 1611 bp
• Locus ID: 15212
• UniProt ID: P20060
• Cytogenetics: 13 50.66 cM
• Gene Summary: Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues.[UniProtKB/Swiss-Prot Function]