Adgrg5 (NM_001145972) Mouse Untagged Clone

CAT#: MC217539

Gpr114 (untagged) - Mouse G protein-coupled receptor 114 (Gpr114), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adgrg5
• Synonyms: Gm1109; Gpr114; PGR27
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217539 representing NM_001145972
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCACACGGCGCCCTTTTCTTCTACCTGTGCCTTCTGGCTGCTCAAGTTGTCCTAGTAGAAACCC
  TGTCAGACCTACTTGTCTTGATGAAGCGCCTTGAGCAGCCCGTAGGCCGGGGCTTGTCTTCCAGAGCGAG
  GCATATCCACAGCCTGGAGCAGAAGCTGCTCAATGCCAGCTTCGGCGGGCACAACTTGACCTTGCAGACA
  AACTCCATCCAGTCTCTTGTCTTCAAGCTGAGCTGCGACTTTCCTGGTCTTTCCCTGTCCAGCACCACAC
  TGACAAATGTCTCCCAGGTGCGAGCCCCACATGCCATGCAATTCCCTGCTGAGCTGACCAAGGGTGCCTG
  TGTGACCTCCCGGCCTGCTGAGCTTCGACTCATCTGCATCTATTTCTTCACTGCACACCTCTTTCAGGAT
  GACCGGAACTCATCACTGCTCAATAACTATGTCCTGGGGGCCCAGCTGGATCACAGGCCTGTGAACAACC
  TTCAGAAGCCAGTCAACATCAGCTTCTGGCATAATCGGAGTCTGGAAGGGTATACAGTATCCTGTGTTTT
  CTGGAAGGAGGGAGCCAGCAAGAGCAGCTGGGGGGCCTGGAGCCCTGAGGGCTGTTATACAGAGCAGCCC
  TCAGCCACCCAGGTTCTCTGCCATTGCAACCATCTCACCTACTTCGCTGTGCTTATGCAGCTCTCTGGAG
  ACCCCGTGCCCGCTGAGCTGCAGGTGCCTCTTGAGTACATCTCCTTTGTGGGTTGCAGCATCTCCATCGT
  GGCCTCGCTGCTCACCATACTGCTGTACGCTCAGTCCAGGAAGCAAAGCGATTCCACCACACGTATCCAC
  ATGAACCTGAATGGCTCTGTTCTGCTCCTGAACGTCACCTTCCTTCTGAGCTCCCAGATGACCCTGCCCA
  CCATGCCCAGGCCCGTCTGCAAGGTGCTGGCTGCTGTCCTACACTACGCACTGCTCAGCAGCCTTACCTG
  GATGGCCATCGAAGGCTTCAACCTCTACCTTTTCCTGGGGCGTGTCTACAACGCCTACATTCGCCGATAC
  TTGCTCAAGCTCTGCATGCTGGGCTGGGGGTTTCCAGCCCTCTTGGTGCTGCTGCTTCTGATGATCAAGA
  GCTCAGTGTATGGACCCTGTGTGACCTCACTCTCCAAAAGCCAGGAAAATGGCACAGGCTTCCAGAATGT
  GTCCATGTGCTGGATCCGAAGTCCCATGGTACACAGCATCCTGGTCATGGGCTATGGTGGCTTCACATCT
  CTGTTCAACCTGGTGGTGCTGGCCTGGGCTCTGTGGATCTTGTGCAGGCTTCGGGCACGGGAGAAGGCAC
  TGAGTCCCTGGGCCTACCGGGACACTGCCATGGTGTTGGGTCTCACTGTGCTGCTTGGCACCACCTGGAC
  CCTAGCCTTCTTCTCCTTCGGTGTGTTCCTGCTGCCTCAGCTCTTCCTCTTTACCATCTTCAACTCGCTC
  TATGGTTTCTTCCTCTTCCTGTGGTTCTGCTCACAGAAGCGTTACTCGGATGCCGAAGCCAAGGCAGAGA
  TGGAGGCAGTCAGCTCCTCCCAGATGACACACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001145972
• Insert Size: 1575 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC145803, AAI45804
• RefSeq Size: 1694 bp
• RefSeq ORF: 1575 bp
• Locus ID: 382045
• UniProt ID: Q3V3Z3
• Cytogenetics: 8 C5
• Gene Summary: Adhesion G protein-coupled receptor (GPCR). Transduces intracellular signals through coupling to guanine nucleotide-binding protein G(s) subunit alpha and activation of adenylate cyclase pathway (PubMed:22575658). Isoform 1, but not isoform 2, is constitutively active, as evidenced by elevated basal cAMP levels, and responds to mechanical activation (shaking) (PubMed:26499266).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1).