Snx27 (NM_029721) Mouse Untagged Clone

CAT#: MC217483

Snx27 (untagged) - Mouse sorting nexin family member 27 (Snx27), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Snx27
• Synonyms: 5730552M22Rik; ESTM45; ESTM47; R75405
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217483 representing NM_029721
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACGAGGACGGGGAAGGTATTCACCCCTCAGCCCCGCACCGGAATGGAGGCGGCGGCGGGGGGT
  CTGGGCTCCACTGCGCCGGGAACGGTGGCGGGGGAGGCGGCGGCCCGCGGGTCGTGCGCATTGTCAAGTC
  CGAATCCGGCTACGGCTTCAACGTGCGGGGCCAAGTGAGCGAGGGCGGGCAACTGCGGAGCATCAACGGG
  GAGCTGTACGCGCCGCTGCAGCATGTGAGCGCCGTGCTGCCCGGGGGGGCGGCCGACCGGGCCGGGGTGC
  GCAAAGGGGACCGCATCCTGGAGGTGAACGGCGTGAATGTTGAGGGGGCGACACACAAGCAGGTGGTGGA
  CCTGATCCGAGCAGGCGAGAAGGAATTGATCTTGACAGTGTTGTCTGTACCTCCTCATGAGGCTGATAAC
  CTAGATCCCAGTGACGACTCCTTGGGACAATCATTTTATGATTACACAGAAAAGCAAGCAGTGCCCATAT
  CGGTCCCCACATACAAACATGTGGAGCAGAATGGTGAGAAGTTTGTGGTATACAATGTTTACATGGCTGG
  GAGGCAGCTGTGTTCTAAGCGGTACCGGGAGTTTGCCATACTGCACCAAAACCTGAAGAGAGAATTTGCC
  AACTTTACATTTCCTCGACTTCCAGGAAAGTGGCCATTCTCATTATCAGAACAACAATTGGATGCCCGGC
  GTCGTGGGTTGGAAGAATATCTAGAAAAGGTGTGTTCAATCCGAGTCATTGGTGAGAGTGACATCATGCA
  GGAATTCCTGTCTGAGTCTGATGAGAATTACAATGGTGTGTCTGATGTAGAACTGAGAGTAGCACTACCG
  GATGGAACAACGGTTACTGTCAGAGTTAAGAAGAACAGTACTACAGACCAAGTGTACCAGGCTATTGCAG
  CAAAGGTTGGCATGGACAGTACAACAGTGAATTACTTTGCCTTATTTGAAGTGATCAATCATTCCTTTGT
  ACGTAAGCTGGCACCTAATGAATTTCCTCATAAACTCTACGTCCAGAACTATACATCAGCTGTGCCAGGC
  ACTTGCCTGACCATTCGCAAGTGGCTCTTTACAACAGAAGAAGAAGTCCTCTTAAATGACAATGACCTTG
  CTGTTACGTACTTCTTTCATCAGGCTGTTGATGATGTAAAGAAAGGTTACATTAAAGCAGAAGAGAAATC
  TTACCAGTTACAGAAGCTCCATGAACAAAGGAAGATGGTCATGTACCTCAACATGCTAAGGACCTGCGAG
  GGCTACAATGAAATCATCTTCCCTCACTGTGCCTGTGACTCGAGGAGGAAGGGACATGTTATCACAGCCA
  TCAGCATCACGCACTTTAAACTTCATGCATGCACTGAAGAAGGGCAGCTGGAGAACCAGGTAATAGCGTT
  TGAATGGGATGAGATGCAGCGATGGGACACAGATGAAGAAGGAATGGCCTTCTGTTTTGAATATGCGCGA
  GGAGAGAAGAAGCCTCGATGGGTTAAAATCTTCACACCATATTTCAATTACATGCATGAATGCTTTGAGA
  GGGTGTTCTGCGAGCTCAAGTGGAGAAAAGAGGAATATTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029721
• Insert Size: 1581 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029721.2, NP_083997.1
• RefSeq Size: 6273 bp
• RefSeq ORF: 1581 bp
• Locus ID: 76742
• UniProt ID: Q3UHD6
• Cytogenetics: 3 F2.1
• Gene Summary: Involved in the retrograde transport from endosome to plasma membrane, a trafficking pathway that promotes the recycling of internalized transmembrane proteins. Following internalization, endocytosed transmembrane proteins are delivered to early endosomes and recycled to the plasma membrane instead of being degraded in lysosomes. SNX27 specifically binds and directs sorting of a subset of transmembrane proteins containing a PDZ-binding motif at the C-terminus: following interaction with target transmembrane proteins, associates with the retromer complex, preventing entry into the lysosomal pathway, and promotes retromer-tubule based plasma membrane recycling. SNX27 also binds with the WASH complex. Interacts with membranes containing phosphatidylinositol-3-phosphate (PtdIns(3P)). May participate in establishment of natural killer cell polarity. Recruits CYTIP to early endosomes.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate 3' terminal exon which results in a frameshift compared to variant 1. It encodes isoform 2 which has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.