Tsen54 (NM_029557) Mouse Untagged Clone

CAT#: MC217480

Tsen54 (untagged) - Mouse tRNA splicing endonuclease 54 homolog (S. cerevisiae) (Tsen54), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tsen54
• Synonyms: 0610034P02Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217480 representing NM_029557
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCGAACCCGAACCCGGGTCGGTGGAGGTTCCCGCGGGGCGTGTGCTCAGCGCCTCGGAGCTCC
  GCGCCGCGCGCTCGCGGTCCCAGAAGCTACCACAGCGGTCGCATGGCCCCAAGGACTTCCTCCCGGACGG
  CTCGGAGGCCCAGGCGGAGCGGCTGCGCCTGTGTCGCCAAGAGCTGTGGCAGCTGCTGGCGGAGGAGCGC
  GTGGAGCGCCTGGGCAGCTTGGTGGCCGCGGAGTGGAAACCAGAAGAGGGCTTTGTGGAGTTGACGTCTC
  CTGCGGGGAAATTCTGGCAGACCATGGGCTACTCAGAGGAAGGACGGCAGCGACTTCACCCCGAAGAGGC
  TTTGTATCTGCTGGAGTGTGGTTCGATTCAGCTCTTCTACCAAGACCTCCCACTGTCTATCCAAGAGGCA
  TACCAGCTGCTGCTGACAGAGGACACCTTGAGTTTCCTGCAGTACCAGGTCTTCAGCCACCTGAAGAGAC
  TGGGCTATGTGGTTCGTCGGTTCCAGCTAAGCTCTGTCGTGTCACCTTACGAGCGGCAGCTTAATTTGGA
  TGGTTATGCCCAGTGCCTGGAGGATGGGTCTGGCAAGAGGAAGAGGAGTTCCAGCTGTCGGTCTGTTAAT
  AAGAAGCCCAAGGTCCTGCAGAACTCCCTGCCGCCCGTCAGCCTGGCAGCCTCCAGCTCACCTGCCTGTG
  ACCAGAGTAGCCAATACCCAGAGGAGAAGTCTCAGGACTCAAGCCCCAGGCAGGGCTCAGAGCTCCCCTT
  GCAGTTCCTGGGGTCCTCAGAGCCTTGCTCTGATCTGGCCAGGGAAGACGTGGGGTGTGACCGAGAGAGT
  CACAAAATAGAGAATGGAGCTAAGGGAACCCCTAAGCTACGCTGGAACTTTGAGCAGATCTCATTCCCCA
  ACATGGCCTCTGATAGTCGCCACACTTTCCTGCCTGCTCCAGCCCCAGAGCTGCTCCCAGCCAATGTCAT
  TGGGCGAGGGACAGATGCTGAGTCCTGGTGCCAAAAGCTGAACCAGCGGAGGGAGAAGCTCTCCCGTAGA
  GACCGGGAACAGCAAGCAGTGGTCCAGCAGTTCCGGGAGGATGTTAACGCAGATCCCGAGGTGCGGGGCT
  GCTCCAGCTGGCAGGAGTACAAGGAGCTGCTGCAGAGGCGACAGACGCAGAAGAGCCAGCCCCGGCCTCC
  ACACCTATGGGGCCAGTCCGTTACCCCCTTGCTCGACCCTGATAAAGCCGATTGTCCAGCCGCGGTCCTG
  CAACATATCTCTGTACTGCAGACGACACACCTTGCTGATGGAGGTTACCGGCTGCTGGAGAAGTCTGGAG
  GCTTGCAGATCAGCTTCGATGTTTACCAGGCTGACGCTGTGGCGACGTTCCGAAAAAACAGCCCTGGCAA
  ACCCTATGTGCGAATGTGCATTAGTGGGTTTGATGACCCTGTCCCAGACCTCTGCAGCCTCAAGTGCTTG
  ACCTACCAGAGTGGGGATGTTCCCCTGATCTTTGCTCTAGTGGACCACGGTGACATCTCCTTCTACAGCT
  TCAGAGACTTCACACTGCCCAGGGATCTGGGCCACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029557
• Insert Size: 1578 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029557.1, NP_083833.1
• RefSeq Size: 1972 bp
• RefSeq ORF: 1578 bp
• Locus ID: 76265
• UniProt ID: Q8C2A2
• Cytogenetics: 11 E2
• Gene Summary: Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events (By similarity).[UniProtKB/Swiss-Prot Function]