Gmcl1 (NM_011818) Mouse Untagged Clone

CAT#: MC217449

Gmcl1 (untagged) - Mouse germ cell-less homolog 1 (Drosophila) (Gmcl1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gmcl1
• Synonyms: 2810049L19Rik; Btbd13; DIP; Gcl; Gcl-1; glc-1; mglc-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217449 representing NM_011818
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGCTCTCAGCAGCCGGGTGCTGCGGCCCGCAGGGCGCACAGAGCAGCCCGAACCCACGCCCGGGG
  CTGGGGGCGCGGCCCGCAGGTCGGACGCCGGCGAAGATGCGGGCCACAGCTTCTGTTACTGTCCGGGCGG
  CCGCAAGCGCAAGCGCAGCAGCGGCACATTCTGCTACTGTCACCCCGACTCCGAGACAGACGACGACGAG
  GACGAGGGCGACGAGCAGCAGAGGCTGCTGAACACGCCGCGCAGGAAAAAATTAAAGAGCACATCAAAAT
  ACATCTACCAAACGCTGTTTTTGAATGGTGAAAACAGTGACATTAAGATCTGTGCTCTAGGTGAAGAGTG
  GAGCTTACACAAAATCTACTTATGTCAATCTGGCTACTTTTCTAGTATGTTCAGTGGTTCTTGGAAAGAA
  TCCAGCATGAATATTATTGAACTGGAGATTCCTGACCAGAACATTGATATAGAAGCACTGCAGGTCGCAT
  TTGGATCACTGTATCGAGATGACGTCTTAATAAAGCCCAGCAGGGTCGTTGCCATTTTGGCAGCAGCTTG
  CATGCTGCAATTGGATGGTTTGATACAGCAGTGCGGTGAGACAATGAAGGAGACCATCTCTGTGAGAACT
  GTGTGTGGCTATTACACATCGGCAGGGACCTATGGACTAGACTCTGTAAAGAAAAAGTGCCTCGAGTGGC
  TGCTGAACAACCTCATGACTCACCAGAGTGTGGAGCTTTTCAAAGAACTCAGTATAAACGTCATGAAACA
  GCTCATTGGTTCGTCTAACTTATTTGTGATGCAAGTGGAGATGGATGTATATACAGCTCTTAAAAAGTGG
  ATGTTCCTTCAGCTGGTGCCTTCCTGGAATGGGTCTTTAAAGCAGCTTTTGACAGAAACAGATGTCTGGT
  TTTCAAAGTGGAAAAAAGACTTTGAAGGGACGACTTTCCTTGAAACTGAGCAGGGAAAACCATTTGCGCC
  CGTGTTCAGACATTTAAGGCTACAGTACATTATCAGTGATCTGGCTTCTGCAAGGATCATTGAGCAGGAT
  TCTCTGGTACCTTCAGAATGGCTGGCGGCAGTGTATAAACAGCAGTGGCTGGCTATGCTACGGGCTGAAC
  AAGACAGTGAAGTGGGGCCTCAAGAAATCAATAAAGAAGAACTTGAGGGAAACAGCATGAGGTGTGGTCG
  AAAGCTTGCCAAAGATGGTGAGTACTGCTGGCGCTGGACAGGCTTCAATTTCGGCTTTGACCTCCTTGTG
  ACTTACACCAATCGATACATCATTTTCAAACGCAATACGCTGAACCAGCCATGTAGTGGATCTGTCAGCT
  TACAGCCTCGAAGGAGCATAGCATTTAGATTGCGCTTGGCTTCTTTTGACAGTAGTGGGAAACTCATATG
  CAGTAGAGCAACTGGCTACCAAATACTGACGCTTGAAAAAGACCAGGAGCAAGTGGTGATGAACTTGGAC
  AGCAGACTTCTGATCTTCCCTCTGTACATCTGCTGTAACTTCTTGTATATATCACCAGAAAAAAGAACTG
  AGAGTAATCGTCACCCAGAAAACCCAGGACACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011818
• Insert Size: 1575 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011818.3, NP_035948.3
• RefSeq Size: 2967 bp
• RefSeq ORF: 1575 bp
• Locus ID: 23885
• UniProt ID: Q920G9
• Cytogenetics: 6 37.75 cM
• Gene Summary: Possible function in spermatogenesis. Enhances the degradation of MDM2 and increases the amount of p53 probably by modulating the nucleocytoplasmic transport.[UniProtKB/Swiss-Prot Function]