Txk (NM_013698) Mouse Untagged Clone

CAT#: MC217443

Txk (untagged) - Mouse TXK tyrosine kinase (Txk), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Txk
• Synonyms: A130089B16Rik; Btkl; PTK-RL-18; PTK4; Rlk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217443 representing NM_013698
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCTCCTTTTCAGATAGCTCCTTCCAGTCTGTTCTCTGCTGCTGCTGTTGCCGCTGCTCAGTACAGA
  AGAGACAGGTGAGAACTCAGATAAGCCTGAGCAGAGAGGAAGAACTCTCAGAAAAACATTCCCAGCGTCA
  GAGGCCGTGGTTCGCCAAACTGATGGGCAAAACTCAATCCAACAGAGGCGGGGTGCAACCCTCGAAGCGC
  AAGCCGCTGCCCCCCCTCCCGCAGGAGCCTCCAGATGAGAGAATCCAGGTCAAGGCTCTTTATGACTTCC
  TGCCTCGGGAGCCTGGTAATTTGGCACTGAAGAGAGCGGAGGAATATCTGATATTGGAGAGGTGTGATCC
  TCACTGGTGGAAGGCCAGAGACCGCTTCGGGAATGAAGGCTTAATCCCAAGCAACTATGTGACAGAAAAC
  AGACTCGCCAACTTAGAAATCTATGAATGGTACCACAAGAACATTACGAGAAACCAGACCGAACGCCTAT
  TGAGGCAAGAGGCTAAAGAAGGTGCCTTTATCGTGAGAGATTCGAGACACTTGGGGTCTTACACAATCTC
  TGTGTTTACAAGAGCTCGAAGGCATACACAGTCTTCAATAAAACATTATCAGATAAAAAAGAATGACTCC
  GGACAGTGGTACATCACCGAAAGACATCTCTTCCCCTCAGTCCCCGAGTTGATCCAGTATCACCAGTACA
  ATGCAGCTGGTCTCATATCTCGTCTCCGCTATCCCATTGGGCTCCTGGGCAGCTGTTTACCAGCCACATC
  TGGTTTTAGCTATGAAAAGTGGGAGATAGATCCATCAGAGTTGGCTTTTGTCAAGGAGATCGGAAGTGGT
  CAGTTTGGGGTTGTCCACTTAGGAGAATGGAGAGCACATATCCCGGTCGCCATCAAGGCCATCAATGAAG
  GTTCCATGTCTGAAGAAGACTTCATTGAGGAAGCCAAGGTGATGATGAAACTGTCACATTCGAGGTTAGT
  TCAACTTTACGGGGTGTGTATACAGCAGAAGCCCCTGTACATAGTGACGGAGTTCATGGAGAACGGCTGC
  CTGCTTGACTATCTCAGGGAGAGGAAAGGCCAGCTTCAGAAGGCGCTGCTCTTGAGCATGTGCCAAGACA
  TATGTGAAGGGATGGCGTACCTGGAGAGGAGCTGCTATATTCACAGGGATCTGGCTGCCAGGAACTGTTT
  GGTCAGTTCTGCCTGCGTAGTAAAGATCTCAGACTTCGGCATGGCGAGGTATGTTTTGGACGATGAATAT
  ATCAGTTCTTCTGGAGCTAAGTTCCCAGTCAAGTGGTGCCCACCTGAAGTCTTTCATTTCAACAAATACA
  GTAGCAAGTCTGATGTCTGGTCGTTCGGAGTTTTAATGTGGGAAGTTTTTACAGAAGGAAAAATGCCTTT
  TGAAAATAAGTCAAATTTGCAAGTGGTGGAAGCCATTTCTCAAGGTTTCCGGCTGTATCGTCCTCACCTG
  GCCCCCATGACCATATACAGAGTGATGTACAGTTGCTGGCATGAGAGCCCTAAAGGCCGTCCGACATTTG
  CTGAGCTGCTTCAGGTTCTCACGGAGATCGCAGAAACGTGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013698
• Insert Size: 1584 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013698.3, NP_038726.2
• RefSeq Size: 2344 bp
• RefSeq ORF: 1584 bp
• Locus ID: 22165
• Cytogenetics: 5 38.44 cM
• Gene Summary: Non-receptor tyrosine kinase that plays a redundant role with ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of TXK to the cell membrane, where it is phosphorylated at Tyr-420. Phosphorylation leads to TXK full activation. Contributes also to signaling from many receptors and participates in multiple downstream pathways, including regulation of the actin cytoskeleton. Like ITK, can phosphorylate PLCG1, leading to its localization in lipid rafts and activation, followed by subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. With PARP1 and EEF1A1, TXK forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFNG to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Phosphorylates both PARP1 and EEF1A1. Phosphorylates also key sites in LCP2 leading to the up-regulation of Th1 preferred cytokine IL-2. Phosphorylates 'Tyr-201' of CTLA4 which leads to the association of PI-3 kinase with the CTLA4 receptor.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus but is the same size as isoform 1.