Rag2 (NM_009020) Mouse Untagged Clone

CAT#: MC217436

Rag2 (untagged) - Mouse recombination activating gene 2 (Rag2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rag2
• Synonyms: Rag-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217436 representing NM_009020
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCTGCAGATGGTAACAGTGGGTCATAACATAGCCTTAATTCAACCAGGCTTCTCACTTATGAATT
  TTGATGGCCAAGTTTTCTTCTTTGGCCAGAAAGGCTGGCCTAAGAGATCCTGTCCTACTGGAGTCTTTCA
  TTTTGATATAAAACAAAATCATCTCAAACTGAAGCCTGCAATCTTCTCTAAAGATTCCTGCTACCTCCCA
  CCTCTTCGTTATCCAGCTACTTGCTCATACAAAGGCAGCATAGACTCTGACAAGCATCAATATATCATTC
  ACGGAGGGAAAACACCAAACAATGAGCTTTCCGATAAGATTTATATCATGTCTGTCGCTTGCAAGAATAA
  CAAAAAAGTTACTTTCCGTTGCACAGAGAAAGACTTAGTAGGAGATGTCCCTGAACCCAGATACGGCCAT
  TCCATTGACGTGGTGTATAGTCGAGGGAAAAGCATGGGTGTTCTCTTTGGAGGACGTTCATACATGCCTT
  CTACCCAGAGAACCACAGAAAAATGGAATAGTGTAGCTGACTGCCTACCCCATGTTTTCTTGATAGATTT
  TGAATTTGGGTGTGCTACATCATATATTCTCCCAGAACTTCAGGATGGGCTGTCTTTTCATGTTTCTATT
  GCCAGAAACGATACCGTTTATATTTTGGGAGGACACTCACTTGCCAGTAATATACGCCCTGCTAACTTGT
  ATAGAATAAGAGTGGACCTTCCCCTGGGTACCCCAGCAGTGAATTGCACAGTCTTGCCAGGAGGAATCTC
  TGTCTCCAGTGCAATCCTCACTCAAACAAACAATGATGAATTTGTTATTGTGGGTGGTTATCAGCTGGAA
  AATCAGAAAAGGATGGTCTGCAGCCTTGTCTCTCTAGGGGACAACACGATTGAAATCAGTGAGATGGAGA
  CTCCTGACTGGACCTCAGATATTAAGCATAGCAAAATATGGTTTGGAAGCAACATGGGAAACGGGACTAT
  TTTCCTTGGCATACCAGGAGACAATAAGCAGGCTATGTCAGAAGCATTCTATTTCTATACTTTGAGATGC
  TCTGAAGAGGATTTGAGTGAAGATCAGAAAATTGTCTCCAACAGTCAGACATCAACAGAAGATCCTGGGG
  ACTCCACTCCCTTTGAAGACTCAGAGGAATTTTGTTTCAGTGCTGAAGCAACCAGTTTTGATGGTGACGA
  TGAATTTGACACCTACAATGAAGATGATGAAGATGACGAGTCTGTAACCGGCTACTGGATAACATGTTGC
  CCTACTTGTGATGTTGACATCAATACCTGGGTTCCGTTCTATTCAACGGAGCTCAATAAACCCGCCATGA
  TCTATTGTTCTCATGGGGATGGGCACTGGGTACATGCCCAGTGCATGGATTTGGAAGAACGCACACTCAT
  CCACTTGTCAGAAGGAAGCAACAAGTATTATTGCAATGAACATGTACAGATAGCAAGAGCATTGCAAACT
  CCCAAAAGAAACCCCCCCTTACAAAAACCTCCAATGAAATCCCTCCACAAAAAAGGCTCTGGGAAAGTCT
  TGACTCCTGCCAAGAAATCCTTCCTTAGAAGACTGTTTGATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009020
• Insert Size: 1584 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009020.3, NP_033046.1
• RefSeq Size: 3393 bp
• RefSeq ORF: 1584 bp
• Locus ID: 19374
• UniProt ID: P21784
• Cytogenetics: 2 53.87 cM
• Gene Summary: Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3.[UniProtKB/Swiss-Prot Function]