Nobox (NM_130869) Mouse Untagged Clone

CAT#: MC217432

Nobox (untagged) - Mouse NOBOX oogenesis homeobox (Nobox), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nobox
• Synonyms: OG2; Og2x
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217432 representing NM_130869
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCTACGGAGAAGCTCTGCAAGAAAATGCAGGGCCAGGAAGCTGGTGACAAGCCCAGGACTGCTG
  CACTGGAGACAGAGGGACCACTGCAGGATTCTGCCCTGCCCATCCAGGATGATCAAGACAAGCAGTCCTC
  ACTCCCCAGGGCTAGCTTGGGGAAGAGGCCACTGTCGAAGACCTCTGAGGAGTTAATGGATGCTGGCACG
  TGCAGAGTGCACAAAGCCCCCACAGCAGCCGCATGTGGTCCCCAATCCGAGGAAGAAGGTTGTTCTCCCC
  CAGAGAGAAAGGCAGAATCGCTGAAGCCATCCATTTCTGCAGTCCCTGGCCAAGCCACCGCCGGTTCCCT
  CAACTCTCATGAAGGGGACCTGAAGAAAGAATCCCTAGAAGTGACCTGTCAATTTCGGAAAAAGACCCGA
  ACCCTGTACCGCTCAGACCAGCTGGAAGAACTGGAGAGGATATTTCAGGAAGACCACTATCCTGACAGTG
  ACAAACGCCATGAGATTTCCCAGATGGTGGGGGTAACCCCCCAAAGAATCATGGTGTGGTTTCAGAACCG
  CAGGGCAAAGTGGAGAAAAGTGGAGAAACTGAACGAGAAGGAAACTAAGAATGGTCCTGCAGCCCCCAGT
  GCTGACAGCAGCCAGCACAGATCTGCTCCTGAGCTCCTAGACCCTATGCCTACAGACCTAGAACCTGGCC
  CTGTTCCTCCAGAGAACATCCTGGATGTCTTTCCTGAGCCCCCCATGCTGCTGACTTCTGAACAGACTCT
  GACCCCCTTTCAGAATAATGAGGGTGCTGAGAGGGTGGCAGTGACTCCACCACTCCTCAGTCCCCCACCT
  ATTCGAAGGGCCAACCTTCCTCTTCCTCTGGGTCCTGTTCAAACTCCTCAAGTACTGCCTCCAATGAGGG
  ACGTTCCTGGCAGTGACAGCATATACAAGGACAAGGCCTATGTGTCCTGGGGCACTAGTATCGCCTCACC
  ACCCACCTACTCAAATTTGGAAGACCTGGGGTCTCAGGACTACCAAGCAAGCAGCCAGTTGGGTTCATTC
  CAGCTCTCCCAGGCTCCACATTTACCCCTTTTCCCATCCCTTCAGTCACAGTTTCCGTATCTGCCCCCCT
  TCCCTTACCCCATCCCAAGCTCAATGCCATTTCTACCACCAGAAGACTCTCTGTTTTCATTTCCTTTTGG
  CTTCAGTGGAGACTCATCACAGGATTATTGCCCAGGACCCCCACCAGGTCAGATCTTGCTGCAGCCACCT
  GCTGAAAACATGGGTACAGGACCCTGGAGTGGCCATTGCTTGCCAGAACCTCCCTTCCCTCGTCCACACT
  ACCCACAGGCTCTGGGACAACCCCTAGGGGCAGAGGGTTACTTTCCTAACCTATTGCCAACTCCATATGC
  TCTGACTATGAGTAAGCAGTCTTCATTAGGCCTCAATGGGCTTCTCGAAGGCACTAGAGTAGAGACCGGG
  TCCTCACTAAGCAAAATGTCAGATGAGCAGACTTCCTCTTCCCTCGAGCAGCCCGCACTAGAGGAGGTCA
  GAGACAAAAATAAGAATAGCCATGCCGCTGGAGCTAAAGAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_130869
• Insert Size: 1584 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130869.3, NP_570939.1
• RefSeq Size: 1893 bp
• RefSeq ORF: 1584 bp
• Locus ID: 18291
• UniProt ID: Q8VIH1
• Cytogenetics: 6 21.17 cM
• Gene Summary: Transcription factor which plays an essential role in postnatal follicle development. Binds preferentially to the DNA sequences 5'-TAATTG-3', 5'-TAGTTG-3' and 5'-TAATTA-3'. Directly regulates the transcription of POU5F1 and GDF9 during early folliculogenesis.[UniProtKB/Swiss-Prot Function]