Runx2 (NM_009820) Mouse Untagged Clone

CAT#: MC217411

Runx2 (untagged) - Mouse runt related transcription factor 2 (Runx2), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Runx2
• Synonyms: AM; AML3; Cbf; Cbfa; Cbfa-1; Cbfa1; LS3; Os; Osf2; PEB; Pebp2a1; PEBP2aA; Pebpa2a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NM_009820.4
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCAAACAGCCTCTTCAGCGCAGTGACACCGTGTCAGCAAAGCTTCTTTTGGGATCCGAGCACCA
  GCCGGCGCTTCAGCCCCCCCTCCAGCAGCCTGCAGCCCGGCAAGATGAGCGACGTGAGCCCGGTGGTGGC
  TGCGCAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAACAACAGCAACAGCAACAACAG
  CAGCAGCAGCAGCAGCAGCAGGAGGCGGCCGCAGCAGCAGCGGCGGCAGCGGCGGCGGCAGCAGCGGCGG
  CGGCCGCAGTGCCCCGATTGAGGCCGCCGCACGACAACCGCACCATGGTGGAGATCATCGCGGACCACCC
  GGCCGAACTGGTCCGCACCGACAGTCCCAACTTCCTGTGCTCCGTGCTGCCCTCGCACTGGCGGTGCAAC
  AAGACCCTGCCCGTGGCCTTCAAGGTTGTAGCCCTCGGAGAGGTACCAGATGGGACTGTGGTTACCGTCA
  TGGCCGGGAATGATGAGAACTACTCCGCCGAGCTCCGAAATGCCTCCGCTGTTATGAAAAACCAAGTAGC
  CAGGTTCAACGATCTGAGATTTGTGGGCCGGAGCGGACGAGGCAAGAGTTTCACCTTGACCATAACAGTC
  TTCACAAATCCTCCCCAAGTGGCCACTTACCACAGAGCTATTAAAGTGACAGTGGACGGTCCCCGGGAAC
  CAAGAAGGCACAGACAGAAGCTTGATGACTCTAAACCTAGTTTGTTCTCTGATCGCCTCAGTGATTTAGG
  GCGCATTCCTCATCCCAGTATGAGAGTAGGTGTCCCGCCTCAGAACCCACGGCCCTCCCTGAACTCTGCA
  CCAAGTCCTTTTAATCCACAAGGACAGAGTCAGATTACAGATCCCAGGCAGGCACAGTCTTCCCCACCGT
  GGTCCTATGACCAGTCTTACCCCTCCTATCTGAGCCAGATGACATCCCCATCCATCCACTCCACCACGCC
  GCTGTCTTCCACACGGGGCACCGGGCTACCTGCCATCACTGACGTGCCCAGGCGTATTTCAGATGATGAC
  ACTGCCACCTCTGACTTCTGCCTCTGGCCTTCCTCTCTCAGTAAGAAGAGCCAGGCAGGTGCTTCAGAAC
  TGGGCCCTTTTTCAGACCCCAGGCAGTTCCCAAGCATTTCATCCCTCACTGAGAGCCGCTTCTCCAACCC
  ACGAATGCACTACCCAGCCACCTTTACCTACACCCCGCCAGTCACGTCAGGCATGTCCCTCGGCATGTCC
  GCCACCACTCACTACCACACGTACCTGCCACCACCCTACCCCGGCTCTTCCCAAAGCCAGAGTGGACCCT
  TCCAGACCAGCAGCACTCCATATCTCTACTATGGTACTTCGTCAGCATCCTATCAGTTCCCAATGGTACC
  CGGGGGAGACCGGTCTCCTTCCAGGATGGTCCCACCATGCACCACCACCTCGAATGGCAGCACGCTATTA
  AATCCAAATTTGCCTAACCAGAATGATGGTGTTGACGCTGACGGAAGCCACAGCAGTTCCCCAACTGTTT
  TGAATTCTAGCGGCAGAATGGATGAGTCTGTTTGGCGGCCATATTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009820
• Insert Size: 1587 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009820.4, NP_033950.2
• RefSeq Size: 5865 bp
• RefSeq ORF: 1587 bp
• Locus ID: 12393
• UniProt ID: Q08775
• Cytogenetics: 17 21.33 cM
• Gene Summary: This gene encodes a member of the runt domain-containing family of transcription factors. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (3) represents use of a distal promoter. It lacks a segment in 5' UTR and encodes the same isoform 1, compared to variant 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.