Prpf4 (NM_027297) Mouse Untagged Clone

CAT#: MC217397

Prpf4 (untagged) - Mouse PRP4 pre-mRNA processing factor 4 homolog (yeast) (Prpf4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Prpf4
• Synonyms: 1600015H11Rik; AI874830; AW047464; bN189G18.1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217397 representing NM_027297
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCCTCACGAGCCTCCTCCACGACAACCAAAACCAAAGCGCCTGACGATTTAGTTGCTCCCGTTG
  TGAAGAAACCACACATCTACTATGGAAGCTTGGAAGAGAAAGAGCGGGAACGCCTGGCCAAAGGAGAGTC
  TGGGATTTTGGGAAAAGAAGGACTCAAAGCCGGCATTGAAGCAGGAAATATTAATATAACATCCGGAGAA
  GTGTTTGAAATTGAGGAGCACATCAGTGAGCGGCAGGCAGAAGTCTTGGCAGAGTTTGAAAGAAGAAAGC
  GAGCCCGGCAGATCAATGTTTCCACAGATGACTCAGAGGTCAAGGCTTGCCTTCGAGCCTTGGGAGAGCC
  TATCACGCTTTTTGGAGAGGGCCCTGCTGAACGAAGAGAAAGGTTAAGAAATATTCTCTCAGTGGTCGGG
  ACTGATGCCTTAAAAAAGACGAAAAAAGATGATGAAAAGTCTAAGAAGTCAAAGGAGGAGTATCAGCAAA
  CCTGGTACCATGAAGGGCCAAATAGCCTGAAGGTGGCCAGATTGTGGATTGCTAATTATTCACTCCCCAG
  GGCAATGAAACGCTTAGAGGAGGCCCGTCTCCATAAGGAGATTCCTGAGACAACCAGAACCTCCCAGATG
  CAGGAGCTGCACAAGTCTCTCCGGTCTTTGAATAATTTCTGCAGTCAGATTGGAGATGACCGGCCGATCT
  CCTACTGCCACTTCAGCCCCAATTCTAAAATGCTAGCCACAGCTTGTTGGAGTGGGCTTTGCAAGCTCTG
  GTCTGTCCCTGACTGCAGCCTCCTTCACACTCTTCGAGGCCATAACACAAATGTAGGAGCAATTGTATTC
  CATCCCAAATCCACTGTCTCGTTGGACCAGAAGGATGTCAACCTGGCCTCTTGTGCTGCTGATGGTTCTG
  TGAAGCTTTGGAGCCTTGACAGCGATGAACCAGTGGCAGATATTGAAGGCCATACAGTGCGTGTAGCTCG
  GGTAATGTGGCATCCATCAGGGCGTTTCCTGGGCACAACCTGTTATGACCGTTCATGGCGCTTATGGGAT
  TTAGAGGCTCAAGAGGAGATCCTGCACCAGGAAGGCCACAGCATGGGTGTGTATGACATTGCCTTCCATC
  AAGATGGCTCTTTGGCTGGCACTGGGGGACTGGATGCATTTGGCCGGGTTTGGGACCTACGCACAGGACG
  CTGCATTATGTTCCTGGAAGGCCACCTGAAGGAAATCTATGGGATAAATTTCAGCCCCAATGGCTACCAC
  ATTGCCACTGGCAGTGGTGACAACACCTGCAAAGTGTGGGACCTTCGGCAGCGACGCTGTGTCTACACCA
  TCCCCGCCCATCAGAACTTAGTGACCGGTGTCAAATTTGAGCCTATCCATGGGGACTTCTTGCTCACAGG
  TGCCTATGACAATACAGCCAAGATCTGGACCCACCCAGGCTGGTCCCCACTGAAGACCCTGGCTGGTCAT
  GAGGGCAAAGTGATGGGCCTGGACATTTCTTCTGATGGGCAGCTCATAGCCACGTGCTCCTATGACAGAA
  CCTTCAAGCTCTGGATGGCAGAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027297
• Insert Size: 1566 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027297.3, NP_081573.1
• RefSeq Size: 5237 bp
• RefSeq ORF: 1566 bp
• Locus ID: 70052
• UniProt ID: Q9DAW6
• Cytogenetics: 4 B3
• Gene Summary: Plays role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex).[UniProtKB/Swiss-Prot Function]