Cyp11a1 (NM_019779) Mouse Untagged Clone

CAT#: MC217392

Cyp11a1 (untagged) - Mouse cytochrome P450, family 11, subfamily a, polypeptide 1 (Cyp11a1), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cyp11a1
• Synonyms: cscc; Cyp11a; Cypxia1; D9Ertd411e; P450scc; Scc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217392 representing NM_019779
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGCTAAAGGACTTTCCCTGCGCTCAGTGCTGGTCAAAGGCTGCCAACCTTTCCTGAGCCCTACGT
  GGCAGGGTCCAGTGCTGAGTACTGGAAAGGGAGCTGGTACCTCTACTAGCAGTCCTAGGTCCTTCAATGA
  GATCCCTTCCCCTGGCGACAATGGTTGGCTAAACCTGTACCACTTCTGGAGGGAGAGTGGCACACAGAAA
  ATCCATTACCATCAGATGCAGAGTTTCCAAAAGTATGGCCCCATTTACAGGGAGAAGCTGGGCACTTTGG
  AGTCAGTTTACATCGTGGACCCCAAGGATGCGTCGATACTCTTCTCATGCGAGGGTCCCAACCCGGAGCG
  GTTCCTTGTGCCCCCCTGGGTGGCCTATCACCAGTATTATCAGAGGCCCATTGGGGTCCTGTTTAAGAGT
  TCAGATGCCTGGAAGAAAGACCGAATCGTCCTAAACCAAGAGGTGATGGCGCCTGGAGCCATCAAGAACT
  TCGTGCCCCTGCTGGAAGGTGTAGCTCAGGACTTCATCAAAGTCTTACACAGACGCATCAAGCAGCAAAA
  TTCTGGAAATTTCTCAGGGGTCATCAGTGATGACCTATTCCGCTTTTCCTTTGAGTCCATCAGCAGTGTT
  ATATTTGGGGAGCGCATGGGGATGCTGGAGGAGATCGTGGATCCCGAGGCCCAGCGGTTCATCAATGCTG
  TCTACCAGATGTTCCACACCAGTGTCCCCATGCTCAACCTGCCTCCAGACTTCTTTCGACTCCTCAGAAC
  TAAGACCTGGAAGGACCATGCAGCTGCCTGGGATGTGATTTTCAATAAAGCTGATGAGTACACCCAGAAC
  TTCTACTGGGACTTAAGGCAGAAGCGAGACTTCAGCCAGTACCCTGGTGTCCTTTATAGCCTCCTGGGGG
  GCAACAAGCTGCCCTTCAAGAACATCCAGGCCAACATTACCGAGATGCTGGCAGGAGGGGTGGACACGAC
  CTCCATGACCCTGCAGTGGAACCTTTATGAGATGGCACACAACTTGAAGGTACAGGAGATGCTGCGGGCT
  GAAGTCCTGGCTGCCCGGCGCCAGGCCCAGGGAGACATGGCCAAGATGGTACAGTTGGTTCCACTCCTCA
  AAGCCAGCATCAAGGAGACACTGAGACTCCACCCCATCTCCGTGACCTTGCAGAGGTACACTGTGAATGA
  CCTGGTGCTTCGTAATTACAAGATTCCAGCCAAGACTTTGGTACAGGTGGCTAGCTTTGCCATGGGTCGA
  GATCCGGGCTTCTTTCCCAATCCAAACAAGTTTGACCCAACTCGTTGGCTGGAAAAAAGCCAAAATACCA
  CCCACTTCCGGTACTTGGGCTTTGGCTGGGGTGTTCGGCAGTGTCTGGGCCGGCGGATTGCGGAGCTGGA
  GATGACCATCCTCCTTATCAATCTGCTGGAGAACTTCAGAATTGAAGTTCAAAATCTCCGTGATGTGGGG
  ACCAAGTTCAGCCTCATCCTGATGCCTGAGAACCCCATCCTCTTCAACTTCCAGCCTCTCAAGCAGGACC
  TGGGCCCAGCCGTGACCAGAAAAGACAACACTGTGAACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_019779
• Insert Size: 1581 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019779.3, NP_062753.3
• RefSeq Size: 1774 bp
• RefSeq ORF: 1581 bp
• Locus ID: 13070
• UniProt ID: Q9QZ82
• Cytogenetics: 9 31.63 cM
• Gene Summary: Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone, the precursor of most steroid hormones.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1).