Mia2 (NM_177321) Mouse Untagged Clone

CAT#: MC217381

Mia2 (untagged) - Mouse melanoma inhibitory activity 2 (Mia2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mia2
• Synonyms: Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217381 representing NM_177321
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAAGTCAGTGTTCAAAGAATCCTTCTTTTGGTTGTTTCTCTGGCCAAGTGTCTGGAGGGTACAA
  AGTTGCTGGCACACCTTAAGAAGTGTGGTGACTTGGAATGTGAAACTTTGATCAGCCGAGTCTTAGCCCT
  GAGAGATTACACAGGACCCGACTGTCGGTACCTGAACTTCACTACGGGAGAAGAGATATCTGTTTATGTT
  AAACTTGGAGGAGACAGAGAAGATTTGTGGGCAGGAAGCAAAGGAAAAGACTTTGGATTTTTTCCCAGAG
  ATGCAGTCGAGATTGAAGAGGTGTTCATATCTGAAGAAGTCGAAATGCCAACTAAATCTGACTTTCTTTG
  TCTTCTTGGAGAAGGCTACATATTTGGAAGTGAACAGAGTGAATTAAACAGTGAAGATGATGAAGAACAT
  ATGTACCCATATGAAAAAGATGAAGACCAAAACTATAATATATATGAGGGTGATTTTCAGCCAGAACCTG
  ACTTATATGCAGCTGCTGAAGGGACTTTGTTGGAGGACCAAATTCCAGCATCCGAAGCTCCTGATGATTT
  CCGATTCTCCAGTGAGTGGAAGGCCTGGGAAGGGGCTGGAAGCCAGGGTGGAGGGGAGCAGGATTACACT
  GCAGACTCTGACCAAGACTTGCCATCCCTCAGTAAGCCAGAAAGGCAAGGATGGTTTGGCCTGGGGACAG
  AAGAAGCTGAAGAGAAGGTTTTCGAATCAGATACTGAACCTACACAAGAATTAGCACTAGAAGAGGAGAG
  TGACCTGGAGAAATTACACAGTGGCGAACCCCAAGTGGAACTTGAGCAAGAGCCAAAATCAGAGACATTA
  GAATTCAGTTCAGTGCCGGACGAAGAGTATGAGCTAGAATCTGAGACGGAGAGTATCCTCAAACCCCAAG
  CTTCTGGCTGGTTTGGTGAGGGCCTTACAAGTTATTTAGGTTTTGGAAATGAGGAGGCAGGACTTGAGTT
  ATTGTCCAAAGAAAGCAATCCACCATTACAAGATATTCCCAGCTCTGTTCCACCAGATGAAGAAGTCCCG
  GCTCCATGCAGAGAAATCTCAACAGACAAGGAAGATGCAGTCATTAATGATAGCTCGGTTCTCAGTCCAA
  GCTGGTTTTACTATGGATTTGGTATGCTAGGCTTTACAAATGCCGACGAAGACAACATTGTTTCAGACAA
  AGGAGAAAATGAAGATGGTGAGGTAGATAACCTCAAACATCCTATAGGAAGTGACTTTGACCCTGAAAAG
  GAACAAGAAAGGAAAATAGTAACTGTGGAAACCGAAGACCAGGCAGGTACAGAAAGCGTCTTGGAGAAGA
  CAGACGAGTCTGGTTCCATGCAGTATCTGAAGAAGTTCTTTGATAATCCTTGGGGCTTCCAGAGTCTCCC
  AGAGGACACAGAATTACCATTTTCCAAAAAGATGCTGGATCAAGATGATATAGTAGAAAATGACAAAATT
  GAAGAACTTTCCACTGAAAATTCTCCCACAGGTAGCATGAAAGACCCCGTGATGCTGGCGAGCAGATACG
  TTCTGTCAGGTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177321
• Insert Size: 1554 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177321.2, NP_796295.1
• RefSeq Size: 1628 bp
• RefSeq ORF: 1554 bp
• Locus ID: 338320
• UniProt ID: Q91ZV0
• Cytogenetics: 12 C1
• Gene Summary: This gene encodes a protein that is involved in endoplasmic reticulum-to-Golgi trafficking and regulation of cholesterol metabolism. Three major classes of transcripts are generated from this gene- melanoma inhibitory activity 2-specific transcripts, cTAGE family member 5-specific transcripts and transcripts that include exons from both these transcript species. Additionally, alternative splicing in these transcripts results in multiple transcript variants encoding diverse isoforms. A mutation in this gene (couch-potato or cpto) may result in low levels of plasma cholesterol and triglycerides. [provided by RefSeq, Sep 2016]