Plekha8 (NM_001164361) Mouse Untagged Clone

CAT#: MC217362

Plekha8 (untagged) - Mouse pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 8 (Plekha8), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Plekha8
• Synonyms: 6330400G01; AA517676; BC052360; Fapp-2; Fapp2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217362 representing NM_001164361
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGCGTGCTGTACAAGTGGACCAACTACCTGAGCGGTTGGCAGCCTCGATGGTTCCTTCTCTGTG
  GGGGAATACTGTCCTATTACGACTCTCCTGAGGATGCCTGGAAGGGTTGCAAAGGGAGCATCCAGATGGC
  AGTCTGTGAGATTCAAGTTCATTCCGTGGATAACACTCGCATGGACCTCATCATCCCCGGGGAACAGTAT
  TTCTACTTAAAAGCCAGGAGTGTGGCAGAGAGACAGCGGTGGCTGGTGGCTCTGGGATCTGCCAAGGCCT
  GCCTGACAGATAGTAGGACTCAGAAGGAAAAAGAATTTGCAGAAAACACTGAAAACTTGAAAACCAAAAT
  GTCGGAGCTAAGACTGTACTGTGACCTCCTTGTTCAACAAGTAGATAAAACAAAAGAAGTGGCCACAGCC
  GGTGTCACTGACTCTGAGGAGGGAATTGATGTGGGAACCCTGCTAAAATCGACCTGCAACACTTTCCTGA
  AGACCTTGGAAGAATGCATGCAGATTGCAAACGCAGCCTTTACCTCCGAACTGCTTTACCATACACCCCC
  AGGGTCCCCTCAACTAGCGGTGCTTAAGTCCAGCAAGATGAAACATCCCATCATACCAATTCATAATTCG
  TTGGAAAGGTCGATGGAGTTAAACAGTTGTGAAAATGGATCGCTAAGTATAGAAGTAAATGGTGATGAAG
  AAATCCTAATGAAAACCAAGAGTTCCTTGTATTTGAAGTCCACAGAGGTGGATTGCAGCATCTCCAGTGA
  AGAAAACACAGATGACAATGTGACAGTCCAGGGGGAGATAATGAAGGAAGATGGCGAGGAAAACCTGGAG
  AGTCATGACAAGGATCCGGCACAGCCTGGATCAGACTCGGTTTGCTCGCCAGAATCTCCCTGGGAAGACA
  ATGAAGAAGTTATTCCAACTTTCTTCAGTACTATGAATACCAGCTTTAGTGACATTGAACTTCTGGAGGA
  CAGTGGCATTCCCACAGAAGCATTCTTGGCCTCCTGTTATGCAGTGGTTCCAGTCCTAGACAAACTTGGT
  CCCACAGTGTTTGCTCCTGTTAAGATGGATCTTGTTGGAAATATTAAGAAGGTAAATCAGAAGTACATAA
  CCAACAAGGAAGAGTTTACTACCCTCCAGAAGATAGTGCTGCATGAGGTGGAGGCTGACGTCGCTCAGGT
  TAGGAACTCGGCGACGGAAGCCCTCTTGTGGCTGAAGAGAGGTCTCAAATTTCTAAAGGGATTTTTGACA
  GAAGTGAAGAATGGAGAAAAGGATATCCAGACAGCACTGAATAACGCATATGGCAAAACATTACGGCAAC
  ACCATGGCTGGGTAGTTCGAGGGGTCTTTGCGTTAGCACTGAGGGCAGCGCCATCCTATGAGGACTTTGT
  AGCTGCGCTAACCATAAAAGAAGGCGACCACCAGAAAGAAGCTTTCAGTGCTGGCATGCAGAGGGACCTC
  AGCCTTTACCTCCCCGCTATGGAAAAGCAGCTGGCCATACTGGACACGTTATATGAGATCCACGGGCTGG
  AATCTGATGAGGTGGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001164361
• Insert Size: 1560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164361.1, NP_001157833.1
• RefSeq Size: 6723 bp
• RefSeq ORF: 1560 bp
• Locus ID: 231999
• UniProt ID: Q80W71
• Cytogenetics: 6 B3
• Gene Summary: Cargo transport protein that is required for apical transport from the trans-Golgi network (TGN). Transports AQP2 from the trans-Golgi network (TGN) to sites of AQP2 phosphorylation. Mediates the non-vesicular transport of glucosylceramide (GlcCer) from the trans-Golgi network (TGN) to the plasma membrane and plays a pivotal role in the synthesis of complex glycosphingolipids. Binding of both phosphatidylinositol 4-phosphate (PIP) and ARF1 are essential for the GlcCer transfer ability. Also required for primary cilium formation, possibly by being involved in the transport of raft lipids to the apical membrane, and for membrane tubulation (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.