Lrp4 (NM_001145857) Mouse Untagged Clone

CAT#: MC217359

Lrp4 (untagged) - Mouse low density lipoprotein receptor-related protein 4 (Lrp4), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lrp4
• Synonyms: 6430526J12Rik; D230026E03; mdig; Megf7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217359 representing NM_001145857
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCGGTGGTGGGGCGCGCTGCTGCTTGGCGCCCTGCTCTGCGCACACGGAATAGCCAGCAGTCTGG
  AGTGTGCATGTGGTCGGAGCCACTTCACATGTGCGGTGAGCGCGCTTGGCGAATGTACCTGTATCCCCGC
  CCAGTGGCAGTGTGATGGAGACAATGACTGTGGAGACCACAGCGATGAGGATGGCTGTACGCTGCCTACC
  TGCTCCCCTCTGGACTTCCACTGTGACAACGGCAAGTGCATCCGACGCTCCTGGGTGTGTGACGGGGACA
  ATGACTGTGAAGATGACTCTGATGAGCAGGACTGTCCCCCCCGGGAGTGTGAGGAGGACGAATTCCCCTG
  CCAGAACGGCTACTGCATTCGGAGTCTGTGGCACTGTGATGGGGACAACGACTGTGGTGACAACAGTGAC
  GAGCAATGCGACATGCGCAAGTGCTCAGACAAGGAATTCCGCTGCAGCGACGGCAGCTGCATTGCTGAGC
  ATTGGTACTGCGATGGTGACACAGACTGCAAAGATGGCTCTGATGAGGAAAGCTGCCCTTCAGCAGTACC
  CTCTCCTCCTTGCAACCTGGAAGAGTTCCAGTGCGCCTATGGCCGCTGCATCCTCGACATCTACCACTGT
  GACGGAGATGACGACTGTGGAGACTGGTCAGACGAGTCTGACTGCTCCTCCCACCAGCCCTGCCGCTCTG
  GGGAATTCATGTGTGACAGTGGCCTGTGCATCAACTCGGGCTGGCGCTGTGATGGTGATGCAGACTGTGA
  TGACCAGTCTGATGAACGCAACTGCACCACCTCCATGTGCACAGCCGAACAGTTTCGCTGTCGGTCAGGC
  CGTTGCGTCCGTCTGTCCTGGCGCTGTGATGGGGAGGATGACTGTGCGGACAACAGCGATGAAGAAAACT
  GTGAGAACACAGGAAGCCCCCAGTGCGCCTCTGACCAGTTCCTGTGTTGGAACGGGCGCTGTATTGGGCA
  GAGGAAGCTGTGCAACGGGATCAACGACTGCGGTGACAGCAGTGACGAAAGTCCACAGCAGAACTGTCGG
  CCCCGGACGGGTGAGGAGAACTGCAATGTTAACAACGGTGGCTGTGCCCAGAAGTGTCAGATGGTGCGAG
  GGGCAGTGCAGTGTACCTGCCACACAGGCTACCGGCTCACAGAGGATGGGAGAACGTGCCAGGATGTAAA
  CGAATGTGCTGAGGAGGGGTACTGCAGCCAGGGCTGCACAAACACTGAAGGGGCTTTCCAGTGCTGGTGT
  GAAGCAGGGTATGAGCTACGGCCCGATCGGCGCAGCTGCAAAGCTCTGGGGCCAGAACCTGTGCTACTGT
  TTGCCAATCGTATCGACATCCGGCAGGTGCTCCCGCACCGCTCCGAGTACACGCTGCTACTGAACAACCT
  GGAGAACGCCATTGCCCTCGACTTTCATCACCGGCGGGAGCTGGTCTTCTGGTCGGATGTCACCCTGGAC
  CGCATCCTCCGTGCCAACCTTAATGGCAGCAATGTAGAGGAGGTGGTGTCTACTGGGCTAGAGAGCCCAG
  GTAGGAAAACAAGGCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001145857
• Insert Size: 1560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145857.1, NP_001139329.1
• RefSeq Size: 3246 bp
• RefSeq ORF: 1560 bp
• Locus ID: 228357
• UniProt ID: Q8VI56
• Cytogenetics: 2 50.63 cM
• Gene Summary: Mediates SOST-dependent inhibition of bone formation. Functions as a specific facilitator of SOST-mediated inhibition of Wnt signaling. Plays a key role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between motor neuron and skeletal muscle. Directly binds AGRIN and recruits it to the MUSK signaling complex. Mediates the AGRIN-induced phosphorylation of MUSK, the kinase of the complex. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Alternatively, may be involved in the negative regulation of the canonical Wnt signaling pathway, being able to antagonize the LRP6-mediated activation of this pathway. More generally, has been proposed to function as a cell surface endocytic receptor binding and internalizing extracellular ligands for degradation by lysosomes. Plays an essential role in the process of digit differentiation (PubMed:16517118).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1.