Amigo2 (NM_178114) Mouse Untagged Clone

CAT#: MC217341

Amigo2 (untagged) - Mouse adhesion molecule with Ig like domain 2 (Amigo2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Amigo2
• Synonyms: AI415330; Ali1; AMIGO-2; AW208913
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217341 representing NM_178114
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTTAAGGTTCCACACACTGCCCACCCTGCCTAGAGCTGTCAAACCGGGTTGCAGAGAGCTGCTGT
  GTCTGTTGGTGATCGCAGTGATGGTGAGCCCCAGCGCCTCAGGAATGTGCCCCACTGCTTGCATCTGTGC
  CACCGACATTGTCAGCTGCACCAACAAAAACCTATCTAAGGTGCCCGGGAACCTTTTCAGACTGATTAAA
  AGACTGGATCTGAGCTATAACAGAATCGGACTGTTGGATGCCGACTGGATCCCGGTGTCGTTTGTCAAGC
  TGAGCACCTTAATTCTTCGCCACAACAACATCACCAGCATCTCCACGGGCAGTTTCTCCACAACCCCAAA
  TTTAAAGTGTCTGGACTTATCATCCAATAGGCTGAAGTCGGTAAAGAGTGCCACATTCCAAGAGCTGAAG
  GCTCTGGAAGTACTGCTGCTGTACAACAACCACATTTCCTATCTGGACCCCGCAGCGTTCGGGGGGCTTT
  CCCACTTGCAGAAACTCTATCTGAGTGGGAACTTTCTCACACAGTTCCCTATGGATTTGTATACTGGGAG
  GTTCAAGCTGGCTGATCTGACATTTTTAGATGTTTCCTATAATCGAATCCCTTCCATACCGATGCACCAT
  ATAAACTTAGTGCCGGGGAGACAGCTGAGAGGCATCTACCTTCACGGGAACCCATTTGTATGTGACTGTT
  CTCTGTACTCGTTGCTGATCTTTTGGTACCGTAGGCACTTTAGCTCCGTGATGGATTTTAAGAATGACTA
  TACCTGTCGCCTGTGGTCTGACTCCAGGCACTCCCACCAGCTGCAGCTGCTCCAGGAGAGCTTTCTGAAC
  TGTTCTTACAGCGTTATCAACGGCTCCTTCCACGCACTTGGCTTTATCCACGAGGCTCAGGTTGGGGAGA
  GGGCGATCGTCCACTGTGACAGCAAGACTGGCAATGGAAATACTGATTTCATCTGGGTCGGTCCCGATAA
  CAGGCTGCTGGAGCCAGATAAAGACATGGGGAACTTTCGTGTGTTTTACAACGGAAGTCTGGTCATAGAG
  AACCCTGGCTTTGAGGACGCCGGGGTATATTCTTGTATCGCAATGAACAGGCAGCGGCTGTTAAACGAGA
  CGGTGGATATCATGATCAACGTGAGCAATTTCACCATAAACAGATCCCACGCCCACGAGGCGTTTAACAC
  GGCCTTTACCACCCTGGCTGCCTGCGTGGCCAGTATAGTTCTAGTGCTACTGTATCTGTACCTGACGCCG
  TGCCCATGCAAATGCAAAGCCAAGAGACAGAAAAACACGCTGAGCCAAAGCAGTGCCCACTCGTCCATTC
  TCAGTCCTGGCCCCACTGGCGATGCCTCTGCTGACGATCGGAAGGCAGGTAAAAGAGTCGTGTTTCTGGA
  GCCCCTGAAGGACACGGCGGCCGGACAGAATGGCAAAGTCAAGCTTTTCCCCAGTGAGACCGTTATAGCC
  GAGGGCATCTTAAAGTCCACCAGGGCAAAGTCTGACTCAGACTCAGTCAATTCCGTGTTCTCAGACACAC
  CCTTTGTGGCATCCACTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_178114
• Insert Size: 1560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178114.4, NP_835215.1
• RefSeq Size: 3214 bp
• RefSeq ORF: 1560 bp
• Locus ID: 105827
• UniProt ID: Q80ZD9
• Cytogenetics: 15 F1
• Gene Summary: Required for depolarization-dependent survival of cultured cerebellar granule neurons. May mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. May contribute to signal transduction through its intracellular domain (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. COMPLETENESS: complete on the 3' end.