Plin1 (NM_175640) Mouse Untagged Clone

CAT#: MC217340

Plin1 (untagged) - Mouse perilipin 1 (Plin1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Plin1
• Synonyms: 6030432J05Rik; Peri; Plin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217340 representing NM_175640
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAATGAACAAGGGCCCAACCCTGCTGGATGGAGACCTCCCTGAGCAGGAGAACGTGCTCCAGAGAG
  TTCTGCAGCTGCCTGTGGTGAGCGGGACCTGTGAGTGCTTCCAGAAGACCTACAACAGCACCAAAGAAGC
  CCACCCCCTGGTGGCCTCTGTGTGCAATGCCTATGAGAAGGGTGTACAGGGTGCCAGCAACCTGGCTGCC
  TGGAGCATGGAGCCGGTGGTCCGTCGGCTGTCCACCCAGTTCACAGCTGCCAATGAGTTGGCCTGCAGAG
  GCCTGGACCACCTGGAGGAAAAGATCCCGGCTCTTCAATACCCTCCAGAAAAGATCGCCTCTGAACTGAA
  GGGCACCATCTCTACCCGCCTTCGAAGCGCCAGGAACAGCATCAGTGTGCCCATTGCAAGCACCTCTGAC
  AAGGTTCTGGGGGCCACTCTGGCCGGCTGCGAGCTTGCCTTGGGGATGGCCAAAGAGACAGCAGAATATG
  CCGCCAACACCCGGGTTGGCCGACTGGCCTCTGGAGGGGCTGATCTGGCTCTGGGAAGCATCGAGAAGGT
  GGTAGAGTTCCTCCTGCCACCAGACAAGGAGTCAGCCCCTTCTTCCGGACGGCAGAGGACCCAGAAGGCT
  CCCAAGGCCAAACCAAGCCTTGTGAGGAGGGTCAGCACCCTGGCCAACACTCTTTCTCGACACACCATGC
  AAACCACAGCATGGGCCCTGAAGCAGGGCCACTCTCTGGCCATGTGGATCCCGGGTGTGGCACCCCTGAG
  CAGCCTGGCCCAGTGGGGCGCATCGGCAGCCATGCAGGTGGTGTCCCGGCGGCAGAGTGAGGTGCGGGTG
  CCCTGGCTGCACAACCTGGCAGCCTCTCAGGATGAGAGCCATGACGACCAGACAGACACAGAGGGAGAGG
  AGACAGACGACGAGGAGGAGGAAGAAGAGTCCGAGGCTGAGGAGAACGTGCTCAGAGAGGTTACAGCCCT
  GCCCAACCCGAGAGGCCTCCTGGGTGGTGTGGTACACACCGTGCAGAACACTCTCCGGAACACCATCTCC
  GCAGTGACCTGGGCACCTGCGGCTGTGCTGGGCACGGTGGGAAGGATCCTGCACCTCACACCAGCCCAGG
  CTGTCTCCTCTACCAAAGGGAGGGCCATGTCCCTATCCGATGCCCTGAAGGGTGTTACGGATAACGTGGT
  AGACACTGTGGTACACTATGTGCCGCTTCCCAGGCTGTCCCTGATGGAGCCCGAGAGCGAATTCCGAGAC
  ATCGATAACCCTTCAGCAGAGGCGGAGCGCAAAGGGTCCGGGGCGCGGCCCGCCAGCCCGGAGTCCACCC
  CGCGCCCGGGCCAGCCCCGCGGCAGCTTGCGCAGCGTGCGGGGTCTCAGCGCGCCCTCCTGCCCCGGCCT
  GGACGACAAAACCGAGGCGTCAGCGCGTCCCGGCTTCCTGGCTATGCCCAGAGAGAAGCCTGCGCGCAGA
  GTCAGCGACAGCTTCTTCCGGCCCAGCGTCATGGAGCCCATCCTGGGCCGCGCGCAGTACAGCCAGCTGC
  GCAAGAAGAGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175640
• Insert Size: 1554 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175640.2, NP_783571.2
• RefSeq Size: 1935 bp
• RefSeq ORF: 1554 bp
• Locus ID: 103968
• UniProt ID: Q8CGN5
• Cytogenetics: 7 D2
• Gene Summary: Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness (By similarity). Plays a role in unilocular lipid droplet formation by activating CIDEC. Their interaction promotes lipid droplet enlargement and directional net neutral lipid transfer. May modulate lipolysis and triglyceride levels.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein.