Kat5 (NM_178637) Mouse Untagged Clone

CAT#: MC217336

Kat5 (untagged) - Mouse K(lysine) acetyltransferase 5 (Kat5), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kat5
• Synonyms: AI839539; CPLA2; Htatip; Htatip1; PLIP; Tip55; Tip60
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217336 representing NM_178637
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGGTGGGGGAGATAATCGAGGGCTGCCGCCTGCCCGTGCTGCGGCGCAACCAGGACAACGAAG
  ATGAGTGGCCCCTGGCTGAGATCCTGAGCGTGAAGGACATCAGTGGCCGAAAGCTTTTCTATGTCCATTA
  CATTGACTTCAACAAACGTCTGGATGAATGGGTGACTCACGAGCGGCTGGACTTAAAGAAGATCCAATTT
  CCCAAGAAAGAGGCCAAGACACCTACCAAGAACGGACTTCCTGGGTCCCGCCCCGGCTCTCCCGAAAGAG
  AGGTGCCGGCCTCCGCCCAGGCCAGCGGGAAGACCTTGCCAATCCCGGTCCAGATCACACTCCGCTTCAA
  CCTGCCCAAGGAGCGGGAGGCCATCCCAGGTGGCGAGCCTGACCAGCCGCTCTCCTCCAGCTCCTGCCTG
  CAACCCAACCACCGCTCAACGAAACGGAAGGTGGAGGTGGTTTCACCAGCAACCCCAGTGCCCAGCGAGA
  CAGCCCCAGCCTCGGTTTTCCCTCAGAATGGGTCAGCCCGTAGGGCAGTGGCAGCCCAGCCTGGACGGAA
  GCGGAAATCTAATTGCTTGGGCACTGATGAGGATTCTCAGGACAGCTCAGATGGAATACCGTCAGCACCA
  CGAATGACTGGCAGTCTGGTGTCTGACCGGAGCCACGACGACATTGTCACCCGGATGAAGAACATTGAGT
  GTATTGAGCTTGGCCGGCACCGCCTCAAGCCGTGGTACTTCTCCCCGTACCCACAAGAGCTTACCACGCT
  ACCCGTCCTCTACCTGTGCGAATTTTGCCTCAAATATGGCCGTAGCCTCAAGTGTCTGCAACGCCACTTG
  ACCAAATGTGATCTTCGGCACCCTCCAGGCAATGAAATTTACCGCAAGGGCACCATCTCCTTTTTTGAGA
  TTGATGGACGGAAAAACAAGAGTTACTCACAAAACCTGTGTCTTCTGGCCAAGTGTTTCCTGGACCACAA
  AACACTGTACTATGACACTGACCCCTTCCTCTTCTACGTAATGACGGAGTATGACTGCAAAGGTTTCCAC
  ATCGTGGGCTACTTCTCCAAGGAAAAGGAATCCACAGAAGATTACAATGTGGCCTGCATCTTGACTCTGC
  CTCCCTACCAGCGCCGGGGCTATGGCAAGCTGCTTATTGAGTTCAGCTATGAACTCTCGAAAGTAGAAGG
  GAAGACCGGAACTCCTGAGAAACCCCTGTCAGATCTTGGCCTCCTATCCTACCGAAGTTACTGGTCCCAA
  ACCATCTTGGAGATCCTGATGGGGCTGAAGTCGGAGAGCGGGGAGAGGCCACAGATCACCATCAATGAGA
  TCAGTGAAATCACTAGTATCAAGAAAGAAGATGTCATCTCCACACTGCAGTATCTCAACCTCATCAATTA
  CTACAAGGGCCAGTATATCCTAACTCTGTCAGAAGACATCGTGGATGGGCATGAGCGGGCTATGCTCAAG
  CGGCTCCTTCGGATTGACTCCAAGTGTCTGCACTTCACTCCCAAAGACTGGAGCAAGAGAGGAAAGTGGT
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_178637
• Insert Size: 1542 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178637.2, NP_848752.1
• RefSeq Size: 2028 bp
• RefSeq ORF: 1542 bp
• Locus ID: 81601
• UniProt ID: Q8CHK4
• Cytogenetics: 19 A
• Gene Summary: Catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A (By similarity). This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription (By similarity). This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair (By similarity). NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage (By similarity). Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome (By similarity). Also acetylates non-histone proteins, such as ATM, NR1D2, RAN, FOXP3, ULK1 and RUBCNL/Pacer (PubMed:22539723). Directly acetylates and activates ATM. Relieves NR1D2-mediated inhibition of APOC3 expression by acetylating NR1D2 (By similarity). Promotes FOXP3 acetylation and positively regulates its transcriptional repressor activity. Acetylates RAN at 'Lys-134' (By similarity). Together with GSK3 (GSK3A or GSK3B), acts as a regulator of autophagy: phosphorylated at Ser-86 by GSK3 under starvation conditions, leading to activate acetyltransferase activity and promote acetylation of key autophagy regulators, such as ULK1 and RUBCNL/Pacer (PubMed:22539723).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1, also known as alpha).