Cfap53 (NM_028948) Mouse Untagged Clone

CAT#: MC217325

Ccdc11 (untagged) - Mouse coiled-coil domain containing 11 (Ccdc11), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cfap53
• Synonyms: 4933415I03Rik; Ccdc11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217325 representing NM_028948
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACAGCCAGCAGTTTGGCACTGTTCCAAGGGAATTTAAGGGCCCCACTCCCAAAGCGGTGATCATAA
  GAGCCAAGCCTCCCAAAGCTCAGAGAGCTGAGCAACATCTAAAAAGAATCCAGCGCAGCTACCACAAGTA
  CCACACTACTTTGGCTTCCATCAAGTCAAATGAGGAGAATCGCCTGAAATGTGACTGGATCCAGCGCAAT
  AACCACAAGACCTTTGACAGTCTTGTGCAAGCGAGAGTCCAGGATGCCATGCAAGGCTTTGTCATCAATA
  CTGAAGAGAGACGCAATAAGCTACGTGAACTTTTGGCATCAGAAGAAAACGAGTATTTTTCTGAAATGCA
  ATTGAAAGGAGAAACCATTGAAGAGAAAAAAGACAAAATGAGAGAGAGAACCAAGTTATTAAGAGAGAAG
  AAGGAAAAGGAGAGGCAGGAGTTTGTAGCTGAAAAGTTAGACCAGCAGTTCAGGGAACGCTGTGAGGAGC
  TCCGCACTAAATTGGCCAGCATCCACGAGAAAAAGGTGGTTGAGGAACGGAATGCGCAGATAGAATTCAA
  CAAGGAGCTGAAGAGGCAAAAGCTGGTGGAAGAGCACCTGTTTGCGAGGCTTTGGGAGGAAGACAGATTA
  GCGAAGGAAAGGCGAGAAGCCCAAGAGGAGAAGAGACAGAGAGAGCTGGTACAAAATACACGCCTGGGTC
  TGGATGCTCAGGTGACCAGCATCCAGGCACAAAGGCAGGGGGCACGAAGGATGAAGGAAGAGGAGGCCCG
  CATCCTGGAGCAGAACAAAGCACAGATCAAACGAGAGGACGAACAAGAAAAACTCCAGAAGCAGAAGAGA
  AGACAGGAAACTAGGAGCAGTTTGAAAAAAGCTGTGCAGGATAAGATAGAGTCTATGCAGCGGGAGTACA
  GAGAGGACCTGGACTTGAACATGAAACTCGTGGGAAGGGCCCTTCAAGACTTACAAGATGAGGCAGATAA
  GAAGAAGCAGAAGAGAGAAGAAATGGGAAGAGAGCAGAAGATATACAATGACTATCTAATGCAGCGACGA
  GAGGAAGAAAAAGCCCAGGAGAAAGAACTCAACAGACTACTGGAGGACATAAAGGCAAAGAAGTTGGCTG
  AGAAGGATCGGGAGCTGGCACTCCAAAGGGCGGCAAGGAAGCAGCTCATGAATGAAGTCATGAATACAAG
  AAAACTCCAAGTTCAAGAAAGATTGCAGCGAAAACTCAGAGAACAAGAAGAACTTGCTCTGCATGAACAG
  CGTATAAGCGAAAGCCTGAAGGTGCTTCACCAGGAAGACATGGAGGACTTTGCCAGGCGCTGTGCTTTGG
  CTGAGGAGTATAGGAATCAACTTCAGATGCAGATTGCCCACCAGCAGCAGGCTCGAGAAGCAGAAAAGGA
  AGAGGAACGCCAGGAATTTGAAGCAGGGTTGGCAGCAAACAAAGCCTGCCTGGACAAAATACAGAGGATC
  CTCTCTGAGAATCAAGCTCTGTCCCAGAATGTCCACCCCATGCGAAGGGGTTACCCTGACAAGCCTCCTC
  TATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028948
• Insert Size: 1545 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028948.2, NP_083224.2
• RefSeq Size: 1876 bp
• RefSeq ORF: 1545 bp
• Locus ID: 74453
• UniProt ID: Q9D439
• Cytogenetics: 18 E2
• Gene Summary: May play a role in the beating of primary cilia and thereby be involved in the establishment of organ laterality during embryogenesis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the shorter, protein-coding transcript. CCDS Note: The coding region has been updated to extend the N-terminus to one that is supported by available conservation data. The use of an alternative upstream start codon would result in a protein that is 86 aa longer.