Spire1 (NM_176832) Mouse Untagged Clone

CAT#: MC217313

Spire1 (untagged) - Mouse spire homolog 1 (Drosophila) (Spire1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Spire1
• Synonyms: 6030430B19Rik; AI415299; AU022898; AW550622; Spir-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217313 representing NM_176832
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACTGCATACATTTCTGACCAAAATTAAGAGTGCAAAGGAGAACCTTAAGAAGATTCAAGAAATGG
  AAAAGGGTGATGAATCTAGCACAGATCTGGAGGACCTGAAAAATGCAGACTGGGCCCGGTTCTGGGTACA
  AGTGATGAGGGATTTGCGAAATGGGGTAAAACTTAAGAAAGTCCAGCAGCGGCAGTACAACCCTCTGCCC
  ATTGAGTACCAACTGACCCCTTATGAGATGCTCATGGACGACATTCGGTGCAAAAGATACACCTTGAGAA
  AAGTAATGGTAAATGGTGACGTCCCCCCTCGGTTGAAAAAGAGTGCTCATGAGGTCATCCTTGACTTTAT
  CAGATCAAGACCCCCTTTAAATCCAGTTTCAGCCAGAAAACTGAAACCAACCCCACCACGGCCACGGAGC
  CTCCATGAAAGAATATTAGAAGAAATTAAAGCAGAAAGAAAGCTTCGACCTGTGTCACCAGAAGAAATTA
  GACGGAGCAGACTAGATGTCACTACGCCAGAATCTCCAAAGAATGTTGGAGAATCATCTATGGTGAATGG
  AGGCTTAACATCTCAAACAAAAGAAAATGGGCTGAGCGCTGCCCAGCAGGGGTCTGCGCAGAGGAAGAGG
  CTCCTCAAAGCCCCAACCCTGGCTGAACTGGACAGCTCTGACTCTGAGGAGGAAAAAAGTCTGCACAAGT
  CAACCAGCAGCAGCAGTGCGTCTCCCTCCCTCTATGAGGATCCAGTACTGGAGGCCATGTGCTCCAGGAA
  GAAGCCTCCAAAATTCTTGCCCATCTCATCAACACCCCAGCCAGAGAGAAGGCAGCCACCGCAGAGGCGC
  CACTCCATTGAGAAGGAAACACCTACGAACGTGAGGCAGTTTCTGCCACCATCCAGGCAGAGCTCACGGT
  CTCTTGAGGAATTCTGCTACCCAGTGGAGTGCCTCGCTCTCACTGTGGAGGAAGTGATGCACATCCGTCA
  GGTCCTGGTGAAAGCAGAGCTGGAAAAGTACCAACAGTATAAGGATGTCTACACTGCCCTGAAAAAAGGA
  AAGCTCTGCTTCTGTTGCCGAACCCGGAGGTTTTCCTTCTTCACCTGGTCTTACACCTGTCAGTTCTGTA
  AGAGGCCCGTGTGCTCACAGTGTTGCAAAAAGATGCGGCTGCCTTCCAAGCCATACTCTACTCTACCTAT
  CTTCTCTTTGGGACCTTCTGCCTTGCAAAGGGGGGAAAGCTGTTCGAGGTCAGAAAAACCCTCCACTAGT
  CACCATCGGCCTCTTCGAAGTATTGCCAGGTTCTCCACAAAATCCAGGTCTGTGGACAAGTCCGATGAGG
  AGCTCCAGTTTCCCAAAGAGCTCATGGAGGACTGGAGCACGATGGAGGTGTGTGTGGACTGCAAAAAGTT
  CATCTCCGAGATCATCAGCTCAAGCCGGCGCAGCCTGGTGCTGGCTAACAAAAGAGCCAGGTTGAAAAGG
  AAGACACAGTCTTTCTACATGTCCTCGGCGGGCCCCTCGGAGTACTGCCCTTCAGAACGGACTATCAACG
  AGATCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_176832
• Insert Size: 1548 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_176832.2, NP_789802.1
• RefSeq Size: 4761 bp
• RefSeq ORF: 1548 bp
• Locus ID: 68166
• Cytogenetics: 18 E1