Edc3 (NM_153799) Mouse Untagged Clone

CAT#: MC217226

Edc3 (untagged) - Mouse enhancer of mRNA decapping 3 homolog (S. cerevisiae) (Edc3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Edc3
• Synonyms: AA517853; Lsm16; Yjdc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217226 representing NM_153799
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTATGGATTGGCTAGGCAGTATTGTGTCCATCAACTGTGGAGATAGCCTGGGCGTCTACCAGGGAA
  GAGTATCAGCAGTGGATCAGGTCAGCCAGACCATTTCTCTTACTCGGCCCTTCCACAATGGGGTGAAGTG
  TCTTGTACCAGAAGTCACCTTTCGGGCAGGTGATATTACAGAATTAAAAATTTTGGAGATACCAGGTCCT
  GGAGACAACCAGCAGGTTGGAGACCTTCATCAGACAGAATTAGGCCCTTCTGGTGTTGGGTACCAAATGA
  GTATCAGTCAGAATGGAACAGGCAAGGTGGTCAAGAAGCCAGCCTCTTCCAGTAGTGCCCCTCAGAGCAT
  CCCTAAGAGGACAGATGTGAAGAGCCAGGATGTTGCCATTTCTCCTCAGCAGCAGCAGTGCTCAAAGAGT
  TATGTGGACAGGCACATGGAATCTTTGAGTCAGTCCAAAAGCTTCCGTCGTCGGCACAACTCCTGGTCAT
  CTAGTAGCAGGCACCCAAACCAGGCAACTCCAAAGAAAAGTGGTTTAAAAAATGGCCAGATGAAGAATAA
  AGATGACGAGTGCTTTGGAGACGATATCGAGGAGCTCCCAGACACGGACTTTGACTTTGAGGGGAACCTG
  GCCCTGTTTGACAAGGCGGCTGTGTTTGAGGAGATCGACACTTACGAGAGGAGAAGTGGCTCCCGTTCTC
  GGGGTGTCCCAAATGAACGTCCTGCTAGGTACCGCCATGATGAAAACATCCTGGAATCGGAGCCGATTGT
  GTACCGTAGGATCACAGTACCACACAGCGTGAGCAAGGAGTTCTGCACGGACTCTGGCCTGGTAGTCCCA
  AGTGTTTCCTATGAGCTACATAAAAAGCTGTTGTCTGTGGCTGAGAAGCATGGGCTGACCTTAGAGCGGA
  GACTTGAGATGACTGGTGTGTGTGCCAGTCAGATGGCGCTGACTCTCCTTGGAGGACCCAACAGGTTGAA
  TCCTAAGAATGTCCACCAGAGGCCCACAGTAGCTCTCTTGTGCGGCCCCCATGTGAAGGGGGCTCAGGGC
  ATCAGTTGTGGGAGGCACCTCGCCAACCATGATGTGCAAGTCATCCTCTTCCTGCCCAACTTTGTCAAGA
  TGCTGGAGTCTATTACCAACGAGCTGTCTCTCTTCAGCAAGACCCAAGGGCAGCAAGTGTCTAGCCTAAG
  AGATCTCCCTGCTAGCCCTGTGGACCTGGTTATCAACTGCCTGGATTGTCCTGAGAATGCCTTCCTGCGG
  GATCAGCCCTGGTACAAGGCAGCTGTGGCCTGGGCCAACCAGAACCGGGCACCAGTGCTCAGCATAGACC
  CTCCTGTGCATGAAGTTGAACAGGGCATTGATGCCAAATGGTCCCTGGCACTGGGCCTGCCTCTGCCACT
  CGGGGAGCACGCAGGCCGAGTCTATTTGTGTGACATTGGCATTCCCCAGCAAGTCTTCCAGGAGGTTGGC
  ATCAACTACCATTCACCTTTTGGCTGCAAATTTGTCATTCCCCTACACTCTGCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_153799
• Insert Size: 1527 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153799.3, NP_722494.1
• RefSeq Size: 3343 bp
• RefSeq ORF: 1527 bp
• Locus ID: 353190
• UniProt ID: Q8K2D3
• Cytogenetics: 9 B
• Gene Summary: Binds single-stranded RNA. Involved in the process of mRNA degradation and in the positive regulation of mRNA decapping (By similarity).[UniProtKB/Swiss-Prot Function]