Ccdc61 (NM_001033314) Mouse Untagged Clone

CAT#: MC217208

Ccdc61 (untagged) - Mouse coiled-coil domain containing 61 (Ccdc61), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ccdc61
• Synonyms: C530028I08Rik; Gm159
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217208 representing NM_001033314
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCAGCCCGCTGGCCTGCAGGTGGACTACATCTTCCGGGGTGTGGAGCACGCTGTGCGGGTTGTGG
  TTTCTGGGCAGGTGCTGGAGCTGGAGGTGGAGGACCGGATGACAGCTGACCAGTGGCGTGGAGAGTTTGA
  TGCCAACTTCATTGAAGACTTGACTCATAAGACGGGGAACTTTAAACAGTTCAGCATTTTCTGTAACATG
  CTGGAGTCGGCCCTCACCCAGAGCAGTGAGTCTGTCACCCTGGACCTGCTCACCTACACAGACCTGGAGT
  CTCTGCGCAGCCGCAAGCTGGGAGGCCGCCCAGGTGCCTTGGCCCCCAGGTCGGCCCAGCTCAACTCCAA
  ACGCTACCTCATTCTCATCTATTCGGTGGAGTTTGACAGGATTCACTACCCGCTGCCCCTCCCGTACCAG
  GGCAAGCCTGACCCTGTGGTCCTGCAGGGCATCATCCGCTCACTGAAGGAGGAGTTGGGTCATCTTCGAG
  GGCTGAATGGCGGCCAGGATGCTCGGGAGACGGAGATCTGGCACCTGAGGGAGCAGGTGACCCGCTTGAC
  GTCTGAGAAGCGGGAGCTGGAGGCACAGCTGGGCCGCTCCCGAGAGGAGGCTCTGGCGGGGCGGGCAGCA
  CGCCAGGAGGCCGAGTCGTTGCGTGGGCTGGTGAGGGGCCTAGAGCTGGAGTTACGGCAGGAGCGTGGCC
  TCGGGGGTCGGGCTGCTGGCCGCCGCAGCCAGGATAGTCGCCGACTGGCTAAGGAGCTCGAGGAGGTGAA
  GGCATCGGAGCGCAATCTGCGTGCAAGGCTCAAGACACTGAACAGCGAGCTGGCCATGTACAGAAGGGGG
  AGACGCACCTTGCCGCCGGTTGCAGTCAGGGAGGGCCGAGCCTCATCGTCTCGGGAGCGCTCCACGTCCC
  GTGGTCGCGCGGTCACTCGCTCCTCATCACGGGAGAGCAGCCGTGGGGCCCGGGGTCGTGGCCGGCCAGC
  ACACCCCTCGCCCTCACCCACAGGTAGTCGTGCGCCCCGTTTTGACCCCACAGCCTTTGTGAAAGCCAAG
  GAGAAGAAGCAGAGAGAAATCAGGATGAAGCAGCAGCAGCAGCAGCGGAACCGAATGGGCAGTGGAGGAA
  GCGGAGACGGTCCGTCCGTCTCATGGTCTCAGCAGACTCGGCCCGCTGCTGCTGTGACGGGCCGAGGGGA
  CGCAGTTAACCGCTCCCGGAACCGCAGCTCCTCAGTGGACAGTTTCCGCAGCCGCTGCTCGTCAGTCAGC
  TCCTGCAGCGAGCTGGAGGATTTCTCCCACTCAGTTTCCAGAAGTCGCCGCTGCCGTGGCCGAGGGAAAC
  CACCCAGTCCGACACCCTGGAGCAGGTCCAAGACGAAGTCTACCACTCAAGAACGCAGTGATCACCAGAG
  ACACTTGGCCAGTTCTGGGGGCTGGGTCCCTATTAAAGAGTACAGCTCCGACTTCCAGGGAGCTGACATG
  GCTGAAATAGATGCCCGCCTGAAGGCTTTGCAGGAATACATGAACCGGCTGGACACGCGGTCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033314
• Insert Size: 1536 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033314.3, NP_001028486.1
• RefSeq Size: 2010 bp
• RefSeq ORF: 1536 bp
• Locus ID: 232933
• UniProt ID: Q3UJV1
• Cytogenetics: 7 A3
• Gene Summary: Microtubule-binding centrosomal protein required for centriole cohesion, independently of the centrosome-associated protein/CEP250 and rootletin/CROCC linker. In interphase, required for anchoring microtubule at the mother centriole subdistal appendages and for centrosome positioning. During mitosis, may be involved in spindle assembly and chromatin alignment by regulating the organization of spindle microtubules into a symmetrical structure. Plays a non-essential role in ciliogenesis.[UniProtKB/Swiss-Prot Function]