Cgas (NM_173386) Mouse Untagged Clone

CAT#: MC217197

MB21D1 (untagged) - Mouse Mab-21 domain containing 1 (Mb21d1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Symbol: Cgas
• Synonyms: E330016A19Rik; Mb21d1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217197 representing NM_173386
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGATCCGCGTAGAAGGACGACGGCGCCACGCGCTAAGAAGCCGTCCGCGAAGCGCGCCCCGACGC
  AGCCCAGCAGGACCAGGGCCCACGCGGAAAGCTGCGGCCCGCAAAGGGGGGCTCGATCGCGGCGGGCGGA
  GCGTGACGGGGACACCACGGAGAAGCCACGTGCCCCAGGGCCCCGAGTGCATCCAGCAAGGGCCACTGAG
  CTCACCAAAGATGCACAGCCCTCGGCCATGGACGCGGCAGGAGCCACCGCGCGGCCTGCCGTCCGGGTGC
  CCCAGCAGCAGGCCATCCTGGATCCGGAGCTGCCCGCCGTACGGGAGCCCCAGCCGCCCGCGGATCCCGA
  GGCGCGGAAAGTCGTAAGGGGACCTAGCCACAGAAGGGGCGCGCGCTCCACCGGGCAGCCCAGAGCGCCG
  CGAGGGTCCAGGAAGGAACCGGACAAGCTAAAGAAGGTGCTGGACAAATTGAGATTGAAACGCAAAGATA
  TCTCGGAGGCGGCCGAGACGGTGAATAAAGTTGTGGAACGCCTGCTGCGCAGAATGCAGAAACGGGAGTC
  GGAGTTCAAAGGTGTGGAGCAGCTGAACACTGGCAGCTACTATGAACATGTGAAGATTTCTGCTCCTAAT
  GAATTTGATGTTATGTTTAAACTGGAAGTCCCCAGGATTGAGCTACAAGAATATTATGAAACAGGTGCTT
  TCTATCTTGTGAAATTCAAAAGAATTCCACGAGGAAATCCGCTGAGTCATTTCTTAGAAGGGGAAGTATT
  ATCAGCTACCAAGATGCTGTCAAAGTTTAGGAAAATCATTAAAGAAGAAGTTAAAGAAATCAAAGATATA
  GATGTCAGTGTGGAGAAGGAAAAACCAGGAAGCCCTGCTGTAACACTTCTTATCAGGAACCCTGAAGAAA
  TCTCTGTGGATATAATTCTGGCTTTGGAGTCAAAAGGCAGCTGGCCTATTAGTACCAAAGAAGGACTACC
  TATTCAAGGCTGGCTGGGCACAAAAGTGAGGACCAATCTAAGACGAGAGCCGTTTTATCTCGTACCCAAG
  AATGCAAAGGATGGAAATAGTTTTCAAGGAGAGACCTGGCGCCTCTCTTTCTCTCACACTGAAAAGTACA
  TTTTGAATAATCACGGGATAGAGAAAACATGCTGTGAATCTTCCGGAGCAAAATGCTGCAGAAAAGAATG
  TTTAAAATTAATGAAATACCTTTTGGAACAGTTGAAAAAAGAGTTTCAAGAGCTGGATGCATTCTGTTCC
  TACCATGTGAAAACTGCCATCTTTCACATGTGGACCCAGGACCCGCAGGACAGTCAGTGGGACCCCAGGA
  ACCTCAGCTCCTGCTTCGATAAGTTGTTAGCATTCTTTCTTGAGTGCCTCAGGACAGAGAAACTGGATCA
  TTATTTTATTCCAAAGTTCAATCTATTCTCTCAAGAACTAATTGACCGAAAAAGTAAAGAATTTCTATCG
  AAGAAAATTGAATATGAAAGAAATAATGGGTTTCCAATTTTTGACAAGCTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_173386
• Insert Size: 1524 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173386.5, NP_775562.2
• RefSeq Size: 4090 bp
• RefSeq ORF: 1524 bp
• Locus ID: 214763
• UniProt ID: Q8C6L5
• Cytogenetics: 9 E1
• Gene Summary: The protein encoded by this gene is a DNA binding cytosolic protein that catalyzes the synthesis of cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) after sensing the presence of DNA in the cytoplasm. cGAMP binds another protein, Stimulator of interferon genes (STING), leading to the induction of interferons, and a host immune response. Reduced expression of this gene inhibits interferon induction in the presence of some viral infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
  Transcript Variant: This variant (1) encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.