Mier1 (NM_001039081) Mouse Untagged Clone

CAT#: MC217177

Mier1 (untagged) - Mouse mesoderm induction early response 1 homolog (Xenopus laevis (Mier1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mier1
• Synonyms: 4933425I22Rik; 5830411K19Rik; er1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217177 representing NM_001039081
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCCATCTGTTGAGTCTTCAAGTCCAGGAGGCTCAGCAACATCAGAGGACCATGAGTTTGATC
  CATCAGCTGACATGCTGGTTCATGATTTTGATGATGAGCGAACATTAGAAGAAGAAGAAATGATGGAAGG
  AGAGACAAACTTCAGTTCTGAAATAGAGGATCTTGCGAGGGAAGGCGACATGCCAATCCACGAGCTGCTC
  AGCCTCTATGGTTATGACAGCACTGTTCGGTTACCTGAGGAAGAGGAGGAGGAAGAGGAGGAGGAGGAAG
  GTGAAGATGATGAAGATGCTGATAATGATGATAACAGTGGCTGTAGTGGAGAAAATAAAGAAGAGAATAT
  AAAGGATTCATCGGGTCAGGAGGATGAAACTCAGTCTTCCAATGATGATCCCTCACAGTCTGTTACTTCC
  CAAGATGCTCAGGAAATAATCCGCCCACGTCGATGTAAATATTTTGATACAAATAGTGAAATAGAAGAAG
  AATCTGAAGAAGATGAAGATTATATTCCCTCAGAAGACTGGAAAAAGGAAATCATGGTGGGCTCCATGTT
  TCAAGCTGAGATTCCAGTTGGTGTTTGTAGATACAAAGAAAATGAAAAAGTGTATGAAAATGATGATCAG
  CTTCTGTGGGATCCAGAGTGTTTACCAGAAGAGAAAGTGGTTGTCTTTCTTAAGGATGCGTCTAGAAGGA
  CAGGGGATGAGAAAGGTGTGGAAGCAATCCCTGAAGGCTCGCATATCAAAGACAATGAGCAGGCTTTATA
  TGAGTTGGTTAAATGCAGCTTTGATACGGAAGAAGCCTTGAGAAGACTGAGATTTAATGTCAAAGCAGCT
  CGAGAGGAGTTATCTGTGTGGACAGAGGAAGAGTGTAGAAATTTTGAGCAAGGGCTGAAGGCCTATGGAA
  AAGATTTTCATCTGATTCAGGCTAATAAAGTCCGAACAAGATCAGTTGGTGAATGTGTAGCATTCTATTA
  CATGTGGAAAAAGTCTGAGCGCTATGATTTCTTTGCTCAGCAAACAAGGTTTGGAAAAAAGAAATATAAT
  CTTCATCCTGGTGTAACGGATTACATGGATCGTCTTTTGGATGAAAGTGAAAGTGCTGCTTCTAGCCGAG
  CACCATCCCCCCCTCCCACTGCCTCAAATAGCAGTAACAGCCAGTCCGAGAAAGAAGATGGCGCTGTCAG
  CAGTAGGAATCAGAACGGTGTCTCATCTAATGGACCAGGAGAAATACTAAACAAAGAAGAAGTAAAAGTT
  GAAGGGTTACATGTTAATGGACCAACAGGTGGAAATAAGAAACCACTTCTTACAGATATGGACACTAATG
  GTTATGAAGCAAATAACCTGACCACTGACCCAAAACTTGCCCATATGACTGCAAGAAATGAAAATGATTT
  TGATGAAAAAAATGAGAGACCTGCCAAAAGGCGGCGGATAAACAGCAGTGGGAAAGAAAGTCCGGGCTCA
  TCTGAGTTTTTCCAGGAAGCAGTCTCACATGGGAAGTTTGAGGAACATGAAAACACGAATGACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001039081
• Insert Size: 1536 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039081.2, NP_001034170.1
• RefSeq Size: 4650 bp
• RefSeq ORF: 1536 bp
• Locus ID: 71148
• UniProt ID: Q5UAK0
• Cytogenetics: 4 C6
• Gene Summary: Transcriptional repressor regulating the expression of a number of genes including SP1 target genes. Probably functions through recruitment of HDAC1 a histone deacetylase involved in chromatin silencing.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and the 5' coding region and initiates translation at an alternate start codon, compared to variant 4. It encodes isoform b, which is shorter and has a distinct N-terminus, compared to isoform c.