Nsmf (NM_020276) Mouse Untagged Clone

CAT#: MC217166

Nsmf (untagged) - Mouse nasal embryonic LHRH factor (Nelf), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nsmf
• Synonyms: Jacob; Nelf
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217166 representing NM_020276
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGCCGCCGCCTCCAGGAGGAGGGCGCTGAGGAGCGAGGCCATGTCCTCGGTAGCGGCCAAAGTAA
  GAGCAGCCCGAGCGTTTGGAGAGTACCTGTCCCAGAGTCACCCTGAGAACCGCAACGGTGCAGACCACCT
  GCTGGCTGACGCCTACTCTGGCCACGACGGGTCCCCAGAGATGCAACCTGCACCCCAGAACAAGCGCCGC
  CTCTCCCTCGTCTCCAATGGCCGATATGAGGGCAGCATCTCAGATGAGGCAGTCAGCGGGAAGCCGGCTA
  TAGAGGGCCCCCAGCCCCACGTGTACACCATCTCTAGAGAGCCCGCCCTGCTACCTGGCTCTGAAGCTGA
  AGCCATTGAGCTAGCAGTGGTAAAAGGGAGGAGACAGCGGGAACGGCACCCTCACCACCACAGCCAGCCC
  CTGCGTGCCAGCCCAGGCAGCAGCCGGGAGGACATCAGCAGGCCCTGCCAAAGCTGGGCAGGAAGCCGCC
  AGGGCTCCAAAGAGTGCCCAGGATGTGCCCAGCTGGTCCCTGGTCCCTCTTCTCGGGCCTTTGGGCTGGA
  GCAGCCACCTCTACCTGAGGCTCCTGGCCGCCACAAGAAGCTGGAAAGGATGTATAGCGTTGATGGAGTG
  TCTGATGATGTCCCCATCCGTACCTGGTTCCCCAAGGAAAACCTTTTCAGCTTCCAGACGGCAACCACAA
  CTATGCAAGCCATCTCGAACTTCCGCAAACACCTGCGCATGGTTGGCAGTCGGCGGGTGAAGGCCCAGAC
  GTTCGCTGAGCGTCGCGAACGGAGCTTCAGCCGGTCCTGGAGCGACCCCACCCCTATGAAAGCCGACACT
  TCCCACGACTCCCGAGACAGCAGTGACCTACAGAGCTCACACTGCACCCTGGATGAGGCTTGTGAGGACC
  TGGACTGGGACACAGAGAAAGGTCTGGAGGCCATGGCCTGCAACACCGAGGGCTTCTTGCCACCCAAGGT
  CATGCTCATCTCCTCCAAGGTGCCAAAGGCCGAGTATATCCCTACTATCATCCGCAGAGATGACCCGTCC
  ATCATTCCCATCCTCTACGACCACGAGCATGCAACCTTCGAGGACATCCTGGAGGAAATAGAGAAGAAAT
  TGAACATCTATCACAAGGGGGCCAAGATCTGGAAGATGCTGATTTTCTGCCAGGGCGGCCCTGGACACCT
  TTATTTGCTCAAGAACAAGGTGGCCACCTTTGCCAAAGTGGAGAAGGAAGAGGACATGATCCACTTCTGG
  AAGAGGCTGAGCCGCCTGATGAGCAAGGTGAACCCCGAGCCGAATGTCATCCACATCATGGGCTGCTACA
  TTCTGGGGAACCCTAACGGGGAGAAGCTATTCCAGAACCTCAGGACCCTCATGACCCCTTACAAGGTTAC
  CTTTGAGTCGCCTCTCGAGCTGTCTGCCCAAGGGAAGCAGATGATTGAGACCTACTTTGACTTCCGGCTG
  TACCGCCTGTGGAAGAGCCGCCAGCACTCAAAGCTGCTGGACTTTGATGACGTCCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_020276
• Insert Size: 1530 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020276.3, NP_064672.2
• RefSeq Size: 2880 bp
• RefSeq ORF: 1530 bp
• Locus ID: 56876
• UniProt ID: Q99NF2
• Cytogenetics: 2 A3
• Gene Summary: Couples NMDA-sensitive glutamate receptor signaling to the nucleus and triggers long-lasting changes in the cytoarchitecture of dendrites and spine synapse processes. Part of the cAMP response element-binding protein (CREB) shut-off signaling pathway. Stimulates outgrowth of olfactory axons and migration of gonadotropin-releasing hormone (GnRH) and luteinizing-hormone-releasing hormone (LHRH) neuronal cells.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an alternate, in-frame exon, compared to variant 1. The resulting protein (isoform B) is shorter when it is compared to isoform A.