Mitf (NM_001178049) Mouse Untagged Clone

CAT#: MC217141

Mitf (untagged) - Mouse microphthalmia-associated transcription factor (Mitf), transcript variant 3, (10ug)


  "NM_001178049" in other vectors (4)

Reconstitution Protocol

USD 476.00

In Stock*

Size
    • 10 ug

Product Images

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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Mitf
Synonyms BCC2; Bhlhe32; bw; Gsfbcc2; mi; vit; Vitiligo; Wh
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>NM_001178049.1
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCGCTTAGATTTGAGATGCTCATCCCCTGCTCCTTTGAAAGCTTGTGTCTCAGTTCTGCAGAGC
ATTCTGGGGCCTCCAAGCCTCCGTTAAGCTCCTCCACTATGACATCACGCATCTTGCTACGCCAGCAACT
CATGCGTGAGCAGATGCAGGAGCAGGAGCGCAGGGAGCAGCAGCAGAAGCTGCAGGCAGCCCAGTTCATG
CAACAGAGAGTGGCCGTGAGTCAGACACCAGCCATAAACGTCAGCGTGCCCACCACCCTTCCCTCTGCCA
CCCAGGTGCCGATGGAAGTCCTTAAGGTGCAGACCCACCTGGAAAACCCCACCAAGTACCACATACAGCA
AGCTCAGAGGCACCAGGTAAAGCAGTACCTTTCTACCACTTTAGCAAATAAACATGCCAGCCAAGTCCTG
AGCTCACCATGTCCAAACCAGCCTGGCGACCATGCCATGCCACCAGTGCCGGGGAGCAGCGCACCCAACA
GCCCTATGGCTATGCTCACTCTTAACTCCAACTGTGAAAAAGAGGCATTTTATAAGTTTGAGGAGCAGAG
CAGGGCAGAGAGTGAGTGCCCAGGTATGAACACGCACTCTCGAGCGTCGTGCATGCAGATGGATGATGTA
ATTGATGACATCATCAGCCTGGAATCAAGTTATAATGAAGAAATTTTGGGCTTGATGGATCCGGCCTTGC
AAATGGCAAATACGTTACCCGTCTCTGGAAACTTGATCGACCTCTACAGCAACCAGGGCCTGCCACCGCC
AGGCCTTACCATCAGCAACTCCTGTCCAGCCAACCTTCCCAACATAAAAAGGGAGCTCACAGCGTGTATT
TTCCCCACAGAGTCTGAAGCAAGAGCATTGGCTAAAGAGAGGCAGAAAAAGGACAATCACAACTTGATTG
AACGAAGAAGAAGATTTAACATAAACGACCGCATTAAGGAGCTAGGTACTCTGATCCCCAAGTCAAATGA
TCCAGACATGCGGTGGAACAAGGGAACCATTCTCAAGGCCTCTGTGGACTACATCCGGAAGTTGCAACGG
GAACAGCAACGAGCTAAGGACCTTGAAAACCGACAGAAGAAGCTGGAGCATGCGAACCGGCACCTGCTGC
TCAGAGTACAGGAGCTGGAGATGCAGGCTAGAGCGCATGGACTTTCCCTTATCCCATCCACCGGTCTCTG
CTCGCCTGATCTGGTGAATCGGATCATCAAGCAAGAACCAGTTCTTGAGAACTGCAGCCAGGAACTTGTA
CAGCACCAGGCAGACCTGACATGTACGACAACTCTGGATCTCACGGACGGTACCATCACCTTTACCAACA
ACCTCGGCACCATGCCGGAGAGCAGCCCGGCCTACAGCATCCCCAGGAAGATGGGCTCCAACTTGGAAGA
CATCCTGATGGACGATGCCCTCTCACCTGTTGGAGTCACCGACCCACTGCTGTCATCAGTGTCGCCAGGA
GCTTCAAAAACAAGCAGCCGGAGGAGCAGTATGAGCGCAGAAGAAACGGAGCATGCGTGTTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_001178049
Insert Size 1533 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001178049.1, NP_001171520.1
RefSeq Size 4777 bp
RefSeq ORF 1533 bp
Locus ID 17342
Cytogenetics 6 45.05 cM
Gene Summary This transcription factor serves at a critical point between extracellular signaling and downstream targets in cell specification in early eye and neural crest development. Mutant alleles have been identified that generate distinct phenotypes. Some of these alleles are being used to model the human diseases Waardenburg syndrome IIa and Tietz syndrome. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3, also known as Mitf-H) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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