Deup1 (NM_181816) Mouse Untagged Clone

CAT#: MC217122

Ccdc67 (untagged) - Mouse coiled-coil domain containing 67 (Ccdc67), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Deup1
• Synonyms: 4933401K09Rik; Ccdc67
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217122 representing NM_181816
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAACCAAGCCCATACCACAGCAGGAGCTTCTCCTTGTGAAGCTGAGCTTCAGGAACTGATGGAAC
  AAATCGACATCATGGTAAGCAACAAGAAGCTAGACTGGGAAAGGAAGATGCGGGCTTTAGAGACACGGTT
  AGACCTTCGGGATCAAGAGTTGGCCAACGCACAGACTTGCTTGGACCAGAAAGGCCAGGAGGTGGGGTTG
  CTTCGGCAGAAGCTGGACAGCCTAGAGAAATGTAATCTAGTAATGACTCAGAACTATGAGGGGCAGCTAC
  AGACCCTAAAAGCTCAATTTTCTAAGCTAACCAGTAACTTCGAAAAGCTGAGATTACACCAAATGAAGCA
  AAACCAAATTCACCGAAAGGAGTCCTCCAGCAAAGAAGAACTGCCCTTTGAGCTGAGCAGTCTGAACCAG
  AAACTAGAGGAGTTCCGAGCAAAGTCGAGGGAGTGGGACAAGCAGGAGGTCCTCTACCAGACCCACCTGG
  TGTCCCTGGATGCTCAGCAGAAGTTGCTCTCTGAAAAGTGCAGCCAGTTTCAGAAACAGGCACAAAATTA
  CCAAACTCAGCTAAACGGGAAAAAGCAGTGCGCAGAGGACAGCAGCTCTGAGATCCCTCGCCTGGTGTGT
  GAGTCGGATCCTGGCTGTGAAGCCACTCAAAGAGATGAGTTCATTATCGAGAAGTTAAAGTCGGCCGTGA
  GTGAAATCGCGCTGAGCAGGAACAAGTTACAAGATGAAAACCAGAAGCTCCTGCAGGAACTGAAGATGTA
  CCAGAGGCAGTGCCAGGCCATGGAAGCAGGACTGTCAGAGGTGAAGAGTGAGTTACAGTCGCGAGACGAT
  CTCCTGAGGATTATAGAAATGGAAAGACTGCACTTGCATAGAGAACTGCTGAGAATGGGTGAAGTGCAGA
  CTGCTCAAGATAACAGGAAAAGAGTTGAGTCATCATATTCCCCTTCTCCTAAAGAAGCAGAAAGGAAAAG
  GAAGGAGTTGTTTCCAATGGTCTCAGATCAACCCAATCATGAAAAGGAATTAAGCAAGGCTGTGGACTTA
  AGTAACAAGAAGCATTCACAGTGCACATCAATTAACAAGCTTGAATATGAGAACGAAAGACTCCGAAGCG
  ATCTCGCCAAGCTCCATGGCAATGGAAAAGCAGCGTGGCCCAATCAGAGCAGCTATGGTGAAGCAGGGGC
  ATACGTCTTCCAGAGCCAGCTGAAAACGGAGACCAGTGGAGACAGAATTAGCCAAGACTGCGAGCTGAAC
  AGAAGCCCCACACCCTTGTCGCCTTTGCCATTTCAGACCAAAGAAATGGCAAGCCCATTGGTTGGTGACA
  ATGAAGTCCTCGCTCTGTCTCCACCAGATATATCCTTCCGGGCTTCCCTAGCTGCACAGCACTTCCTGAT
  GGAGGAAGAGAGGCGGGCCAAGGAGCTTGAGAAGCTGCTCAACACACACATCGATGAGCTGCAGAGACAC
  ACCGAGTTCACGCTTAACAAATACACCAAACTCAAGCAGAGTAGACACATATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_181816
• Insert Size: 1524 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181816.2, NP_861537.2
• RefSeq Size: 3045 bp
• RefSeq ORF: 1524 bp
• Locus ID: 234964
• UniProt ID: Q7M6Y5
• Cytogenetics: 9 A2
• Gene Summary: Key structural component of the deuterosome, a structure that promotes de novo centriole amplification in multiciliated cells. Deuterosome-mediated centriole amplification occurs in terminally differentiated multiciliated cells and can generate more than 100 centrioles. Probably sufficient for the specification and formation of the deuterosome inner core. Interacts with CEP152 and recruits PLK4 to activate centriole biogenesis.[UniProtKB/Swiss-Prot Function]