Cyp7b1 (NM_007825) Mouse Untagged Clone

CAT#: MC217116

Cyp7b1 (untagged) - Mouse cytochrome P450, family 7, subfamily b, polypeptide 1 (Cyp7b1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cyp7b1
• Synonyms: AW261589; D3Ertd552e; hct-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217116 representing NM_007825
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGGAGCCACGACCCTAGATGCCGCCTCGCCAGGGCCTCTCGCCCTCCTAGGCCTTCTCTTTGCCG
  CCACCTTACTGCTCTCGGCCCTGTTCCTCCTCACCCGGCGCACCAGGCGCCCTCGTGAACCACCCTTGAT
  AAAAGGTTGGCTTCCTTATCTTGGCATGGCCCTGAAATTCTTTAAGGATCCGTTAACTTTCTTGAAAACT
  CTTCAAAGGCAACATGGTGACACTTTCACTGTCTTCCTTGTGGGGAAGTATATAACATTTGTTCTGAACC
  CTTTCCAGTACCAGTATGTAACGAAAAACCCAAAACAATTAAGCTTTCAGAAGTTCAGCAGCCGATTATC
  AGCGAAAGCCTTCTCTGTAAAGAAGCTGCTTACTGATGACGACCTTAATGAAGACGTTCACAGAGCCTAT
  CTACTTCTACAAGGCAAACCTTTGGATGCTCTTCTGGAAACTATGATCCAAGAAGTAAAAGAATTATTTG
  AGTCCCAACTGCTAAAAATCACAGATTGGAACACAGAAAGAATATTTGCATTCTGTGGCTCACTGGTATT
  TGAGATCACATTTGCGACTCTATATGGAAAAATTCTTGCTGGTAACAAGAAACAAATTATCAGTGAGCTA
  AGGGATGATTTTTTTAAATTTGATGACATGTTCCCATACTTAGTATCTGACATACCTATTCAGCTTCTAA
  GAAATGAAGAATCTATGCAGAAGAAAATTATAAAATGCCTCACATCAGAAAAAGTAGCTCAGATGCAAGG
  ACAGTCAAAAATTGTTCAGGAAAGGCAAGATCTGCTGAAAAGATACTATAGGCATGACGATCCTGAAATA
  GGAGCACATCATCTTGGCTTTCTCTGGGCCTCTCTAGCAAACACCATTCCAGCTATGTTCTGGGCAATGT
  ATTATATTCTTCGGCATCCTGAAGCTATGGAAGCCCTGCGTGACGAAATTGACAGTTTCCTGCAGTCAAC
  AGGTCAAAAGAAAGGACCTGGAATTTCAGTCCACTTCACCAGAGAACAATTGGACAGCTTGGTCTGCCTG
  GAAAGCACTATTCTTGAGGTTCTGAGGCTGTGCTCATACTCCAGCATCATCCGAGAAGTGCAGGAGGATA
  TGAATCTCAGCTTAGAGAGTAAGAGTTTCTCTCTGCGGAAAGGAGATTTTGTAGCCCTCTTTCCTCCACT
  CATACACAATGACCCGGAAATCTTCGATGCTCCAAAGGAATTTAGGTTCGATCGCTTCATAGAAGATGGT
  AAGAAGAAAAGCACGTTTTTCAAAGGAGGGAAGAGGCTGAAGACTTACGTTATGCCTTTTGGACTCGGAA
  CAAGCAAATGTCCAGGGAGATATTTTGCAGTGAACGAAATGAAGCTACTGCTGATTATGCTTTTAACTTA
  TTTTGATTTAGAAATTATCGACAGGAAGCCTATAGGGCTAAATCACAGTCGGATGTTTTTAGGTATTCAG
  CACCCCGATTCTGCCGTCTCCTTTAGGTACAAAGCAAAATCTTGGAGAAGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007825
• Insert Size: 1524 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC038810, AAH38810
• RefSeq Size: 1814 bp
• RefSeq ORF: 1524 bp
• Locus ID: 13123
• UniProt ID: Q60991
• Cytogenetics: 3 4.98 cM
• Gene Summary: Oxysterol 7alpha-hydroxylase that mediates formation of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) from 25-hydroxycholesterol (PubMed:9295351). Plays a key role in cell positioning and movement in lymphoid tissues: 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells (PubMed:22999953).[UniProtKB/Swiss-Prot Function]