Trim12c (NM_175677) Mouse Untagged Clone

CAT#: MC217078

Trim12c (untagged) - Mouse tripartite motif-containing 12C (Trim12c), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trim12c
• Synonyms: 9230105E10Rik; Trim12-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217078 representing NM_175677
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCACAATTCATGAAGAATTTAAAGGAGGAAGTGACCTGTCCTCTCTGTCTGAACCTGATGGTGA
  AACCTGTGAGTGCAGATTGTGGTCACAGCTTCTGCCAAGGCTGCATCACGGTGTACTTTGAATCCACCAA
  ATGCGATAAGGAAATGTTCAGTTGCCCTGTGTGCCGACTTAGTTACCAGTCTAGCAATCTGAGGCCTAAT
  CTACATGTGGCCAACATAGTAGAGAGGCTCAAAGAGTTCAAGCCTAGCCCAGAAGAGGAGCAGAAAGTGT
  TTAACTGTGCAAGACATGGAGAGAAACTCCAGCTCTTCTGTAGGAAGGACATGATGGCCATCTGCTGGCT
  TTGTGAGCGATCTCAGGAGCACCGGGGCCACAAAACAGCTCTCATTGAAGAGGTGGCCCAGGAGTACAAG
  GAGCAGCTGCAGGTAGTTCTGCAAAGGCTGATGGCAGACAAGAAAGAATTTGAAAACTGGAAAGATGACC
  TTCAGAAGGATAGAACTTACTGGGAGAATCAAATACAGAAAGATGTGGAGAATGTTCAGTCAGAGTTTAA
  ACGAATGAGGGATATCATGAACTCTGAAGAGAAGAAGGAATTGCAGAAGCTGAAGCAAGAGAAGGAAAAC
  ATTATCAACAAACTGGCAGAGTCTGAAAATGAGCATGCTCAGCAGAGCAAGTTGCTAGAAGACTTCATTT
  CAGATGTGGAACATCAGTTACAGTGCTCAGACATAGAAATACTGCAGGGTGTGGAGAACATCATAAAACG
  GAGTCATACTTTTTCGATGAAGAAGCCCAAAGCCATCGCCAGGGAACAAAGAAAGTTCCGAGCCCCTGAC
  CTGCAAGGCATGCTGCAAGTGCTGCAAGAGGTCACAGAGGCTCAACGCTACTGGGTTCAGGTGACGCTGG
  TTCAAAACAACAATCCAAACATTGCCATTACTGATGACAAAAGGCAAATACGATATGAAGACCTCCAAGC
  AAGGAATCTTGAACATGGCTGTGAAAACTCTCATGAAGGTGTCCTGGGACACCCAGTTATCCAATCAGGA
  AAACATTACTGGGAAGTAGATGTGTCTGGAAAAGGTGCTTGGGTTCTGGGGTTAAGTGATGGAAGCTACC
  TCTTCAATCCAATATTTCGTTCAAATGCTGAAAGAAACCCTTTATTTCGCAGGGGGATTAAGAATGATTC
  ACATTATCAACCAAAATATGGCTTCTGGGTTATAGGGCTGTGGAAAAAGTCTGTGTATAATGCTTTTGAG
  GAGTGTCCTTTCACAGGCAAGCCCAGTGTCTTGACCCTGTCTCTGATGGTCCCGCCCTGTCGTGTTGGTG
  TTTTCCTTGACTATGCAGCTGGGACTCTCTCATTTTACAATATTTCCAACAATGGGACTCTTATCTACAG
  ATTCTGTACAGCTTCCTTTCCTGATAGGGTTTTTCCATATTTTAATCCCATGGGAAGTTCAGAGCCCATG
  ACAATTTGCTGGCCAGACTCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175677
• Insert Size: 1494 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175677.4, NP_783608.3
• RefSeq Size: 3617 bp
• RefSeq ORF: 1494 bp
• Locus ID: 319236
• Cytogenetics: 7 E3