Notum (NM_175263) Mouse Untagged Clone

CAT#: MC217030

Notum (untagged) - Mouse notum pectinacetylesterase homolog (Drosophila) (Notum), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Notum
• Synonyms: 5730593N15Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217030 representing NM_175263
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGGAGAGGTGCGCGTGCTGCTACTGCTGGGCCTGCTGCATTGGGTTGGGGGCAGCGAGGGCAGGA
  AGACCTGGAGGCGCCGTGGCCAGCAGCCACCCCAGCCGCCGCCCCCGCCGCCGCTCCCGCAGCGGGCTGA
  GGTGGAGCCGGGCGCCGGGCAGCCGGTGGAGAGCTTCCCGCTGGACTTCACGGCAGTGGAGGGCAACATG
  GACAGCTTTATGGCGCAAGTCAAGAGCCTGGCACAGTCCCTGTATCCCTGCTCTGCGCAGCAGCTCAATG
  AGGACCTGCGCCTTCACCTCCTGCTGAACACGTCGGTGACCTGCAATGACGGCAGCCCCGCCGGCTACTA
  TTTGAAGGAGTCCAAGGGCAGTCGGCGGTGGTTACTCTTTCTGGAAGGCGGCTGGTATTGCTTCAACCGG
  GAGAACTGTGATTCCAGATACAGCACCATGCGGCGCCTCATGAGCTCCAAAGACTGGCCACACACACGCA
  CAGGTACTGGTATTCTATCCTCCCAGCCAGAGGAGAACCCCCACTGGTGGAATGCCAATATGGTCTTCAT
  CCCTTACTGCTCCAGTGACGTTTGGAGTGGGGCTTCACCCAAGTCTGACAAGAATGAATATGCCTTCATG
  GGCTCCCTCATCATCCAGGAGGTGGTACGGGAGCTCCTGGGCAAAGGGCTGAGCGGGGCAAAGGTGCTGC
  TGTTGGCCGGGAGCAGCGCTGGGGGCACCGGGGTGCTGTTGAATGTAGACCGCGTGGCTGAGCTGCTGGA
  GGAGTTGGGCTACCCGTCTATCCAGGTGCGGGGCCTGGCTGACTCAGGCTGGTTCCTGGACAACAAGCAG
  TACCGGCGATCAGACTGCATTGATACTATCAACTGTGCACCCACAGACGCCATCCGCCGTGGCATCAGGT
  ACTGGAGTGGGATGGTCCCAGAGCGTTGCCAGCGCCAGTTCAAGGAAGGAGAGGAATGGAATTGCTTCTT
  TGGCTACAAAGTATATCCGACTCTGCGCTGTCCGGTGTTCGTGGTACAGTGGCTTTTCGATGAGGCCCAG
  CTTACCGTGGATAACGTGCATCTCACCGGACAGCCGGTTCAGGAAGGCCAGTGGCTATACATCCAGAATC
  TGGGCCGAGAACTGCGTGGTACACTCAAGGATGTGCAGGCCAGCTTTGCCCCCGCCTGCCTGTCTCATGA
  GATCATCATACGGAGCTATTGGACGGACGTCCAGGTGAAGGGGACCTCACTGCCCAGGGCACTGCACTGC
  TGGGACCGGAGCTTCCATGACAGCCATAAGGCCAGCAAAACCCCCATGAAGGGCTGCCCTTTCCACCTGG
  TAGACAGCTGCCCCTGGCCCCACTGCAATCCCTCATGCCCTACCATCCGGGACCAGTTCACAGGGCAAGA
  GATGAACGTGGCACAGTTCCTTATGCATATGGGCTTCGATGTGCAGACGGTGGCTCAGCAGCAGGGGATG
  GAGCCCAGCAAGCTGCTAGGGATGCTGAGTAATGGGAACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175263
• Insert Size: 1512 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175263.4, NP_780472.3
• RefSeq Size: 2020 bp
• RefSeq ORF: 1512 bp
• Locus ID: 77583
• UniProt ID: Q8R116
• Cytogenetics: 11 E2
• Gene Summary: Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors.[UniProtKB/Swiss-Prot Function]