Plag1 (NM_019969) Mouse Untagged Clone

CAT#: MC216999

Plag1 (untagged) - Mouse pleiomorphic adenoma gene 1 (Plag1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Plag1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216999 representing NM_019969
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACTGTCATTCCTGGTGATTTGTCAGAAGTAAGAGATACCCAGAAAGCCCCTTCAGGGAAACGTA
  AGCGTGGTGAAAGCAAACCAAGAAAAAACTTTCCTTGCCAACTGTGTGACAAGGCCTTTAACAGTGTTGA
  GAAATTAAAGGTTCACTCCTTCTCTCACACGGGAGAGAGGCCCTACAAGTGCACACACCAAGACTGCACC
  AAGGCCTTTGTTTCTAAGTACAAATTACAAAGGCACATGGCTACTCACTCTCCTGAGAAAACCCACAAGT
  GTAATTACTGTGAGAAAATGTTTCACCGGAAAGACCATCTGAAGAATCACCTCCATACACATGACCCCAA
  CAAAGAGACCTTTAAATGCGAAGAGTGTGGCAAGAGCTACAATACCAAGCTTGGTTTTAAGCGACACTTG
  GCCTTGCATGCTGCCACCAGTGGTGACCTCACCTGCAAGGTGTGTTTGCAGAATTTTGAGAGCACAGGTG
  TGCTCCTAGAGCACCTGAAATCTCACGCGGGCAAGTCATCTGGGGGTGTGAAGGAGAAAAAGCACCAATG
  TGAGCACTGCGAACGCAGGTTCTACACCCGGAAGGATGTCCGGAGACACATGGTGGTACACACAGGAAGA
  AAGGACTTCCTCTGTCAGTACTGCGCACAGAGATTTGGACGAAAGGATCACCTCACTCGACACATGAAGA
  AGAGTCACAATCAGGAGCTTCTGAAGGTCAAAACGGAACCAGTAGATTTCCTGGACCCATTTACCTGCAA
  CATGTCTGTGCCTATAAAAGATGAACTCCTGCCGGTCATGTCCTTACCTTCCAGTGAACTCTTGTCAAAG
  CCATTCACAAACACTTTGCAGTTAAACCTCTATAACACTCCATTTCAGTCCATGCAGAGCTCTGGGTCTG
  CTCACCAAATGATAACAACTTTACCTTTGGGAATGACATGCCCCATAGATATGGATGCTGTTCACCCCTC
  TCATCATCTTGCTTTCAAATGCCCATTCAGTTCTACCTCATACGCAATCTCTATTCCTGAAAAAGAACAG
  CCATTAAAGGGGGAAATTGAGAGTTACCTGATGGAGCTACAAGGTGGTGCACCATCTTCATCCCAGGATT
  CTCCAGCATCGTCATCTAAGCTAGGGTTAGAGCCTCAGAGTGGGTCGCCAGATGATGGTGCTGGGGACCT
  CTCCCTGTCAAAGAGCTCTATCTCTATCAGTGACCCCCTCAGCACACCAGCATTGGATTTCTCTCAGTTG
  TTCAATTTCATACCATTGAATGGTCCCCCTTATAACCCACTTTCAGTGGGAAGCCTTGGGATGAGCTATT
  CCCAGGAGGAAGCACACTCTTCTGTCTCTCAGCTGCCCACACAAACACAGGATCTTCAGGACCCTGCAAA
  CACTGTGGGTCTAAGTTCTCTGCACTCACTGTCGGCAGCGTTCACCAGCAGCCTGAGCTCAAGCACTACC
  TTGCCCCGTTTCCACCAGGCGTTTCAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_019969
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019969.3, NP_064353.2
• RefSeq Size: 4635 bp
• RefSeq ORF: 1500 bp
• Locus ID: 56711
• UniProt ID: Q9QYE0
• Cytogenetics: 4 A1
• Gene Summary: Transcription factor whose activation results in up-regulation of target genes, such as IGFII, leading to uncontrolled cell proliferation: when overexpressed in cultured cells, higher proliferation rate and transformation are observed. Other target genes such as CRLF1, CRABP2, CRIP2, PIGF are strongly induced in cells with PLAG1 induction. Proto-oncogene whose ectopic expression can trigger the development of pleomorphic adenomas of the salivary gland and lipoblastomas. Cooperates with CBFB-MYH11.[UniProtKB/Swiss-Prot Function]