Adat1 (NM_013925) Mouse Untagged Clone

CAT#: MC216993

Adat1 (untagged) - Mouse adenosine deaminase, tRNA-specific 1 (Adat1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adat1
• Synonyms: mADAT1; MMADAT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216993 representing NM_013925
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGACCGCGGATGAGATCGCTCAGCTGTGCTATGCACACTATAACGTCAGACTGCCCAAGCAGGGGA
  AGCCTGAGCCAAATCGTGAGTGGACCTTGCTGGCAGCTGTGGTGAAGATTCAGGCTTCAGCTAACCAAGC
  TTGTGACATCCCTGAGAAGGAAGTGCAAGTGACAAAGGAAGTTGTCTCAATGGGAACAGGAACAAAATGC
  ATAGGCCAGTCCAAGATGCGAGAGAGCGGGGACATCCTCAATGACAGCCATGCTGAGATCATAGCCAGAA
  GGAGTTTCCAGAGATACCTTCTCCATCAGCTCCATTTGGCAGCCGTCCTGAAAGAGGATAGCATCTTTGT
  TCCAGGGACCCAAAGAGGACTGTGGAGGCTCAGACCTGACCTGTCGTTTGTGTTTTTCTCTAGCCACACA
  CCCTGTGGGGATGCCTCCATCATCCCGATGCTCGAGTTTGAAGAACAGCCTTGCTGTCCTGTCATCAGAA
  GTTGGGCCAACAACTCACCTGTACAAGAGACTGAGAATCTAGAGGATTCTAAAGATAAAAGGAACTGTGA
  AGATCCCGCCAGTCCTGTAGCCAAGAAGATGAGGCTGGGAACACCTGCCAGGTCACTCTCCAATTGTGTA
  GCTCACCATGGGACCCAGGAAAGTGGTCCAGTCAAACCAGATGTCAGCAGCTCTGATCTCACCAAGGAGG
  AACCAGATGCTGCCAATGGAATAGCTTCAGGTAGCTTCAGAGTGGTGGATGTGTACAGGACGGGAGCCAA
  GTGTGTTCCTGGAGAAACTGGAGACTTGAGAGAGCCAGGTGCTGCCTACCACCAGGTGGGGCTACTTCGT
  GTAAAACCTGGCAGGGGAGACAGGACATGCTCCATGTCCTGCAGTGACAAGATGGCAAGGTGGAATGTCC
  TCGGATGCCAGGGCGCACTGCTGATGCACTTCCTGGAGAAGCCTATCTACCTGTCAGCTGTGGTCATCGG
  GAAGTGCCCGTACAGCCAGGAAGCCATGAGGAGAGCATTGACTGGCAGGTGTGAGGAAACCTTGGTTTTA
  CCCAGAGGCTTTGGAGTTCAAGAACTGGAAATACAGCAGTCGGGTTTACTGTTTGAACAGAGCCGCTGTG
  CGGTGCACAGGAAAAGAGGTGACAGTCCAGGCCGACTCGTCCCCTGTGGGGCAGCTATCAGCTGGAGCGC
  TGTCCCTCAGCAGCCCTTGGATGTTACAGCCAATGGTTTTCCACAGGGAACAACAAAGAAAGAGATTGGA
  AGCCCTAGGGCCAGATCCCGAATCAGCAAGGTGGAACTCTTTAGATCATTCCAGAAGCTGCTGAGCAGCA
  TTGCAGATGACGAGCAGCCAGACTCCATCAGGGTAACGAAGAAGCTGGACACCTATCAGGAGTACAAGGA
  TGCTGCGTCCGCCTACCAGGAAGCCTGGGGTGCCCTCCGCAGGATACAGCCGTTTGCGTCCTGGATCAGA
  AACCCACCCGACTACCACCAATTTAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013925
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013925.4, NP_038953.1
• RefSeq Size: 3159 bp
• RefSeq ORF: 1500 bp
• Locus ID: 30947
• UniProt ID: Q9JHI2
• Cytogenetics: 8 E1
• Gene Summary: Specifically deaminates adenosine-37 to inosine in tRNA-Ala.[UniProtKB/Swiss-Prot Function]