Tnfrsf8 (NM_009401) Mouse Untagged Clone

CAT#: MC216978

Tnfrsf8 (untagged) - Mouse tumor necrosis factor receptor superfamily, member 8 (Tnfrsf8), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tnfrsf8
• Synonyms: Cd30; D1S166E; Ki; Ki-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216978 representing NM_009401
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGCCCTACTCACCGCAGCGGGGTTGCTGTTCCTGGGGATGCTTCAAGCCTTCCCAACGGATCGAC
  CACTCAAGACCACCTGTGCCGGAGACCTCAGCCACTACCCAGGGGAGGCTGCCAGGAACTGCTGTTACCA
  GTGCCCCTCAGGGTTGTCTCCGACACAGCCATGCCCACGGGGTCCTGCCCACTGCAGGAAGCAGTGTGCA
  CCTGACTACTACGTCAATGAAGACGGGAAGTGCACAGCCTGCGTGACCTGTTTGCCAGGCCTTGTGGAGA
  AGGCTCCGTGTTCCGGGAACTCTCCTCGAATCTGTGAGTGTCAGCCTGGCATGCACTGCTGCACACCAGC
  AGTCAATTCCTGCGCCCGCTGCAAACTCCACTGTTCTGGAGAGGAGGTTGTCAAGTCTCCAGGCACAGCA
  AAGAAGGACACTATCTGTGAGCTGCCTTCCTCGGGATCTGGTCCCAATTGCTCCAATCCGGGTGACCGCA
  AGACACTTACTAGCCATGCCACTCCTCAGGCCATGCCTACTCTAGAATCCCCAGCCAATGACAGTGCAAG
  GAGCTTGCTGCCAATGCGTGTCACCAACCTTGTGCAGGAAGATGCTACTGAGTTGGTGAAGGTTCCAGAA
  TCTTCCTCGTCCAAGGCAAGGGAGCCCAGTCCAGATCCAGGTAATGCAGAGAAGAATATGACCTTGGAGC
  TTCCATCTCCAGGGACACTCCCTGACATCAGCACCTCAGAAAACAGCAAGGAGCCTGCAAGTACCGCCTC
  CACCCTAAGCCTTGTGGTAGATGCCTGGACCAGCAGCAGGATGCAGCCCACCTCTCCATTGTCCACGGGA
  ACACCATTTCTGGATCCAGGGCCCGTGCTCTTCTGGGTGGCCATGGTGGTGCTACTGGTTGGCTCCGGCT
  CCTTTCTCCTGTGTTACTGGAAGGCCTGTAGGAGGCGGTTCCAGCAGAAGTTTCACCTGGACTACCTAGT
  GCAGACCTTCCAGCCCAAGATGGAGCAGACAGATTCCTGTCCTACTGAAAAGCTAACCCAGCCACAGAGA
  AGCGGGTCGGTGACAGATCCCAGCACGGGACACAAGTTGAGCCCAGTGAGCCCTCCTCCAGCTGTAGAGA
  CTTGTGCCAGTGTTGGGGCCACCTACCTGGAGAACCTGCCGCTGCTGGATGACAGCCCAGCTGGGAATCC
  TTTTTCTCCCAGGGAACCTCCAGAGCCCCGGGTATCCACGGAACACACCAATAACAGGATTGAGAAAATC
  TACATCATGAAGGCCGACACAGTGATCGTGGGCTCTGTAAAAACTGAAGTCCCTGAGGGCCGGGCTCCAG
  CAGGGTCTACAGAGTCTGAGTTGGAAGCTGAACTAGAAGTGGACCATGCCCCCCATTACCCGGAGCAGGA
  GACAGAACCACCTCTGGGCAGCTGCACTGAGGTCATGTTCTCGGTGGAGGAAGGAGGAAAAGAGGACCAT
  GGGCCCACGACTGTCTCTGAGAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009401
• Insert Size: 1497 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009401.2, NP_033427.1
• RefSeq Size: 1575 bp
• RefSeq ORF: 1497 bp
• Locus ID: 21941
• UniProt ID: Q60846
• Cytogenetics: 4 78.17 cM
• Gene Summary: Receptor for TNFSF8/CD30L (PubMed:8543792). May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B (By similarity).[UniProtKB/Swiss-Prot Function]