Nrf1 (NM_001164226) Mouse Untagged Clone

CAT#: MC216967

Nrf1 (untagged) - Mouse nuclear respiratory factor 1 (Nrf1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nrf1
• Synonyms: C87038; D6Ertd415e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216967 representing NM_001164226
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGCACGGAGTGACCCAAACTGAACACATGGCTACCATAGAAGCCCATGCAGTGGCCCAGCAAG
  TCCAGCAGGTCCATGTAGCCACGTACACTGAGCACAGTATGCTAAGTGCTGATGAAGACTCCCCTTCCTC
  CCCCGAGGACACTTCTTATGATGACTCGGACATCCTCAACTCCACGGCAGCTGATGAGGTAACTGCCCAT
  CTGGCTGCTGCAGGTCCTGTGGGAATGGCCGCTGCTGCTGCTGTGGCAACAGGGAAGAAACGGAAACGGC
  CTCATGTGTTTGAGTCTAATCCATCTATCCGAAAGAGACAGCAGACACGTTTGCTTCGGAAACTCAGAGC
  CACGTTGGATGAGTACACGACGCGAGTGGGACAGCAAGCGATTGTACTCTGCATCTCACCCTCCAAACCC
  AACCCTGTCTTCAAGGTGTTTGGCGCAGCACCTTTGGAGAATGTGGTGCGAAAGTACAAGAGCATGATCC
  TGGAAGACCTCGAGTCTGCTCTGGCAGAACACGCCCCTGCGCCACAGGAGGTTAATTCAGAGCTGCCGCC
  TCTCACCATCGATGGGATTCCAGTCTCTGTGGACAAAATGACCCAGGCTCAGCTTCGGGCATTTATCCCA
  GAGATGCTCAAGTATTCCACAGGTCGGGGGAAACCAGGCTGGGGGAAAGAAAGCTGCAAGCCTATCTGGT
  GGCCAGAAGATATCCCATGGGCCAATGTCCGCAGTGATGTCCGCACAGAAGAGCAAAAACAAAGGGTTTC
  ATGGACCCAGGCATTACGGACCATAGTTAAAAATTGCTATAAGCAACATGGGCGGGAGGATCTTTTATAT
  GCTTTTGAAGATCAGCAAACACAAACTCAGGCCACCACCACACACAGTATAGCTCATCTCGTACCATCAC
  AGACCGTAGTACAGACCTTCAGCAACCCTGATGGCACCGTGTCGCTCATCCAGGTTGGTACAGGGGCAAC
  AGTAGCCACATTGGCTGATGCTTCAGAACTGCCAACCACAGTCACTGTTGCCCAAGTGAATTACTCTGCT
  GTGGCTGATGGAGAGGTGGAACAAAACTGGGCCACGTTACAGGGCGGTGAAATGACCATCCAGACGACGC
  AAGCATCAGAGGCCACCCAGGCGGTAGCATCACTGGCAGAAGCCGCAGTGGCAGCTTCTCAGGAGATGCA
  GCAGGGAGCCACTGTCACCATGGCCCTCAACAGTGAAGCTGCCGCCCATGCTGTCGCCACTCTGGCGGAA
  GCCACCTTACAAGGTGGGGGACAGATAGTCCTGTCTGGGGAAACCGCAGCAGCCGTCGGAGCACTTACTG
  GAGTCCAAGATGCTAATGGCCTGGTCCAGATCCCTGTGAGCATGTACCAGACTGTGGTAACCAGCCTCGC
  CCAGGGCAACGGGCCGGTGCAGGTGGCCATGGCCCCAGTGACCACCAGGATATCGGACAGCGCAGTCACC
  ATGGATGGCCAGGCTGTGGAGGTGGTGACCTTGGAACAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001164226
• Insert Size: 1512 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164226.1, NP_001157698.1
• RefSeq Size: 3587 bp
• RefSeq ORF: 1512 bp
• Locus ID: 18181
• UniProt ID: Q9WU00
• Cytogenetics: 6 12.47 cM
• Gene Summary: Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 6 encode the same protein (isoform a).