Kcnj3 (NM_008426) Mouse Untagged Clone

CAT#: MC216960

Kcnj3 (untagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 3 (Kcnj3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kcnj3
• Synonyms: GIRK-1; GIRK1; Kcnf3; Kir3.1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216960 representing NM_008426
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGCACTCCGAAGGAAATTTGGGGACGATTACCAGGTAGTGACCACTTCGTCGAGCGGTTCGGGCT
  TACAGCCTCAGGGGCCAGGACAGGGCCCGCAGCAGCAGCTGGTGCCCAAGAAGAAACGGCAGCGGTTCGT
  GGACAAGAACGGCCGGTGCAATGTGCAGCATGGTAACCTGGGCAGCGAGACCAGTCGCTACCTTTCGGAC
  CTCTTCACTACCCTGGTGGATCTCAAGTGGCGTTGGAACCTCTTTATCTTCATCCTCACCTACACCGTGG
  CCTGGCTCTTCATGGCGTCCATGTGGTGGGTGATCGCTTATACCCGGGGCGACCTGAACAAAGCCCATGT
  CGGCAACTACACTCCCTGTGTGGCCAATGTCTATAACTTCCCCTCTGCCTTCCTCTTCTTCATCGAGACC
  GAGGCCACCATCGGCTATGGCTACCGCTACATCACAGATAAGTGCCCCGAGGGCATCATCCTCTTCCTCT
  TCCAGTCCATCCTTGGCTCCATCGTGGACGCTTTCCTCATCGGCTGCATGTTCATCAAGATGTCCCAGCC
  CAAAAAGCGCGCCGAGACCCTCATGTTTAGCGAGCATGCGGTTATCTCCATGAGGGACGGAAAACTCACT
  CTCATGTTCCGGGTGGGCAACCTGCGCAACAGCCACATGGTCTCCGCGCAGATCCGCTGCAAGCTGCTCA
  AATCTCGGCAGACACCTGAGGGTGAGTTCCTTCCCCTTGACCAACTTGAACTGGATGTAGGTTTTAGTAC
  AGGGGCCGATCAACTTTTTCTTGTGTCCCCTCTCACAATTTGCCACGTGATCGATGCCAAAAGCCCCTTC
  TATGACCTATCCCAGCGAAGCATGCAAACTGAACAGTTCGAGGTTGTCGTCATCCTGGAAGGCATTGTGG
  AAACCACAGGAATGACTTGTCAAGCTCGAACATCATACACAGAGGACGAAGTGCTTTGGGGTCATCGTTT
  TTTCCCTGTAATTTCTTTAGAAGAAGGATTCTTTAAAGTCGATTACTCCCAGTTCCATGCAACCTTTGAA
  GTTCCAACCCCTCCTTACAGCGTGAAAGAGCAGGAGGAAATGCTTCTCATGTCCTCCCCTTTAATAGCAC
  CAGCCATAACCAACAGCAAAGAAAGACACAATTCTGTGGAGTGTTTAGATGGACTAGATGACATTAGCAC
  AAAACTTCCATCGAAGCTGCAGAAAATTACGGGGAGAGAAGACTTTCCCAAAAAACTTCTGAGAATGAGT
  TCTACAACCTCAGAAAAAGCCTATAGTTTGGGTGATTTGCCCATGAAACTCCAACGAATAAGTTCAGTTC
  CTGGCAACTCGGAAGAAAAACTGGTTTCTAAAACCACCAAGATGTTATCTGATCCCATGAGCCAGTCTGT
  GGCCGATTTGCCACCAAAGCTTCAAAAGATGGCTGGAGGACCTACCAGGATGGAAGGAAATCTTCCAGCC
  AAACTAAGAAAAATGAACTCTGACCGTTTCACATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008426
• Insert Size: 1506 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC068167, AAH68167
• RefSeq Size: 1679 bp
• RefSeq ORF: 1506 bp
• Locus ID: 16519
• UniProt ID: P63250
• Cytogenetics: 2 C1.1
• Gene Summary: This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This receptor plays a crucial role in regulating the heartbeat.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1 and 3 both encode the same isoform (1).